Cytochrome P450 1A1, 2E1 and GSTM1 gene polymorphisms and susceptibility to colorectal cancer in the Saudi population

The Saudi population has experienced a sharp increase in colorectal and gastric cancer incidences within the last few years. The relationship between gene polymorphisms of xenobiotic metabolizing enzymes and colorectal cancer (CRC) incidence has not previously investigated among the Saudi population...

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Bibliographic Details
Published in:	Asian Pacific journal of cancer prevention : APJCP Vol. 14; no. 6; pp. 3761 - 3768
Main Authors:	Saeed, Hesham Mahmoud, Alanazi, Mohammad Saud, Nounou, Howaida Attia, Salaby, Manal Ali, Semlali, Abdelhabib, Azzam, Nahla, Aljebreen, Abdeulrahan, Alharby, Othman, Parine, Narasimha Reddy, Shaik, Jilani, Arafaha, Maha
Format:	Journal Article
Language:	English
Published:	Thailand 01-01-2013
Subjects:	Adenocarcinoma - genetics Adenocarcinoma - metabolism Adenocarcinoma - pathology Adult Aged Aged, 80 and over Case-Control Studies Colon - metabolism Colorectal Neoplasms - genetics Colorectal Neoplasms - metabolism Colorectal Neoplasms - pathology Cytochrome P-450 CYP1A1 - genetics Cytochrome P-450 CYP1A1 - metabolism Cytochrome P-450 CYP2E1 - genetics Cytochrome P-450 CYP2E1 - metabolism Female Follow-Up Studies Genetic Predisposition to Disease Genotype Glutathione Transferase - genetics Glutathione Transferase - metabolism Humans Immunoenzyme Techniques Male Middle Aged Neoplasm Staging Polymerase Chain Reaction Polymorphism, Genetic - genetics Polymorphism, Restriction Fragment Length Prognosis Rectum - metabolism Saudi Arabia Survival Rate Tumor Cells, Cultured Saudi Arabia
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Summary:	The Saudi population has experienced a sharp increase in colorectal and gastric cancer incidences within the last few years. The relationship between gene polymorphisms of xenobiotic metabolizing enzymes and colorectal cancer (CRC) incidence has not previously investigated among the Saudi population. The aim of the present study was to investigate contributions of CYP1A1, CYP2E1, and GSTM1 gene polymorphisms. Blood samples were collected from CRC patients and healthy controls and genotypes were determined by polymerase chain reaction restriction fragment length polymorphism and sequencing. CYP2E16 was not significantly associated with CRC development (odd ratio=1.29; confidence interval 0.68-2.45). A remarkable and statistically significant association was observed among patients with CYP1Awt/2A (odd ratio=3.65; 95% confidence interval 1.39-9.57). The GSTM10/0 genotype was found in 2% of CRC patients under investigation. The levels of CYP1A1, CYP2E1 and GSTM1 mRNA gene expression were found to be 4, 4.2 and 4.8 fold, respectively, by quantitative real time PCR. The results of the present case-control study show that the studied Saudi population resembles Caucasians with respect to the considered polymorphisms. Investigation of genetic risk factors and susceptibility gene polymorphisms in our Saudi population should be helpful for better understanding of CRC etiology.
ISSN:	1513-7368 2476-762X
DOI:	10.7314/APJCP.2013.14.6.3761