Search Results - "Sakurai, Takayuki"

Evaluation of antimicrobial stewardship activities using antibiotic spectrum coverage by Tanuma, Michiya, Sakurai, Takayuki, Nakaminami, Hidemasa, Tanaka, Masayo

“…Recently, the days of antibiotic spectrum coverage (DASC) using the antibiotic spectrum coverage (ASC) score was reported as a new tool for measuring…”
Get full text

Contribution of intragenic DNA methylation in mouse gametic DNA methylomes to establish oocyte-specific heritable marks by Kobayashi, Hisato, Sakurai, Takayuki, Imai, Misaki, Takahashi, Nozomi, Fukuda, Atsushi, Yayoi, Obata, Sato, Shun, Nakabayashi, Kazuhiko, Hata, Kenichiro, Sotomaru, Yusuke, Suzuki, Yutaka, Kono, Tomohiro

“…Genome-wide dynamic changes in DNA methylation are indispensable for germline development and genomic imprinting in mammals. Here, we report single-base…”
Get full text

Deficiency of the adrenomedullin-RAMP3 system suppresses metastasis through the modification of cancer-associated fibroblasts by Dai, Kun, Tanaka, Megumu, Kamiyoshi, Akiko, Sakurai, Takayuki, Ichikawa-Shindo, Yuka, Kawate, Hisaka, Cui, Nanqi, Wei, Yangxuan, Tanaka, Masaaki, Kakihara, Shinji, Matsui, Shuhei, Shindo, Takayuki

“…Tumor metastasis is a primary source of morbidity and mortality in cancer. Adrenomedullin (AM) is a multifunctional peptide regulated by receptor…”
Get full text

High-resolution DNA methylome analysis of primordial germ cells identifies gender-specific reprogramming in mice by Kobayashi, Hisato, Sakurai, Takayuki, Miura, Fumihito, Imai, Misaki, Mochiduki, Kentaro, Yanagisawa, Eikichi, Sakashita, Akihiko, Wakai, Takuya, Suzuki, Yutaka, Ito, Takashi, Matsui, Yasuhisa, Kono, Tomohiro

“…Dynamic epigenetic reprogramming occurs during mammalian germ cell development, although the targets of this process, including DNA demethylation and de novo…”
Get full text

Production of genetically engineered mice with higher efficiency, lower mosaicism, and multiplexing capability using maternally expressed Cas9 by Sakurai, Takayuki, Kamiyoshi, Akiko, Kawate, Hisaka, Watanabe, Satoshi, Sato, Masahiro, Shindo, Takayuki

“…The CRISPR/Cas9 system is widely used to generate gene-edited animals. Here, we developed an efficient system for generating genetically modified mice using…”
Get full text

Rac1-Mediated Activation of Mineralocorticoid Receptor in Pressure Overload-Induced Cardiac Injury by Ayuzawa, Nobuhiro, Nagase, Miki, Ueda, Kohei, Nishimoto, Mitsuhiro, Kawarazaki, Wakako, Marumo, Takeshi, Aiba, Atsu, Sakurai, Takayuki, Shindo, Takayuki, Fujita, Toshiro

“…There is increasing evidence for a crucial role of aberrant mineralocorticoid receptor (MR) activation in heart failure, with clinical studies showing…”
Get full text

Predicting the survival of patients with painful tumours treated with palliative radiotherapy: a secondary analysis using the 3-variable number-of-risk-factors model by Sakurai, Takayuki, Saito, Tetsuo, Yamaguchi, Kohsei, Takamatsu, Shigeyuki, Kobayashi, Satoshi, Nakamura, Naoki, Oya, Natsuo

“…The 3-variable number-of-risk-factors (NRF) model is a prognostic tool for patients undergoing palliative radiotherapy (PRT). However, there is little research…”
Get full text

A single blastocyst assay optimized for detecting CRISPR/Cas9 system-induced indel mutations in mice by Sakurai, Takayuki, Watanabe, Satoshi, Kamiyoshi, Akiko, Sato, Masahiro, Shindo, Takayuki

“…Microinjection of clustered regulatory interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9)-related RNA and DNA into fertilized…”
Get full text

Production of single- and multiple-gene-modified mice via maternal SpCas9-based gene editing by Sakurai, Takayuki, Shindo, Takayuki

“…Maternally and transiently accumulated SpCas9 (maternal SpCas9) in a zygote derived from a systemically SpCas9-expressing transgenic mouse strain was used to…”
Get full text

Patterns of failure in glioblastoma multiforme following Standard (60 Gy) or Short course (40 Gy) radiation and concurrent temozolomide by Mizuhata, Miu, Takamatsu, Shigeyuki, Shibata, Satoshi, Sakurai, Takayuki, Minamikawa, Risako, Yamazaki, Masahiro, Kinoshita, Masashi, Miyashita, Katsuyoshi, Kumano, Tomoyasu, Nakada, Mitsutoshi, Gabata, Toshifumi

“…Purpose The purpose of this study was to analyze the patterns of failure in patients with glioblastoma multiforme (GBM) treated using chemoradiotherapy in the…”
Get full text

Bindel-PCR: a novel and convenient method for identifying CRISPR/Cas9-induced biallelic mutants through modified PCR using Thermus aquaticus DNA polymerase by Sakurai, Takayuki, Kamiyoshi, Akiko, Takei, Norio, Watanabe, Satoshi, Sato, Masahiro, Shindo, Takayuki

“…We developed a novel and convenient method for rapidly identifying CRISPR/Cas9-based genome-edited biallelic knockout (KO) cells/individuals carrying…”
Get full text

Adrenomedullin-RAMP2 System in Vascular Endothelial Cells by Koyama, Teruhide, Sakurai, Takayuki, Kamiyoshi, Akiko, Ichikawa-Shindo, Yuka, Kawate, Hisaka, Shindo, Takayuki

“…Vascular endothelial cells play key roles in maintaining vascular and organ homeostasis. Adrenomedullin (AM), originally identified as a vasodilating peptide,…”
Get full text

Role of Adrenomedullin 2/Intermedin in the Pathogenesis of Neovascular Age-Related Macular Degeneration by Kakihara, Shinji, Matsuda, Yorishige, Hirabayashi, Kazutaka, Imai, Akira, Iesato, Yasuhiro, Sakurai, Takayuki, Kamiyoshi, Akiko, Tanaka, Megumu, Ichikawa-Shindo, Yuka, Kawate, Hisaka, Zhao, Yunlu, Zhang, Yan, Guo, QianQian, Li, Peixuan, Onishi, Naho, Murata, Toshinori, Shindo, Takayuki

“…Adrenomedullin 2 (AM2; also known as intermedin) is a member of the adrenomedullin (AM) peptide family. Similarly to AM, AM2 partakes in a variety of…”
Get full text

Adrenomedullin Ameliorates Pulmonary Fibrosis by Regulating TGF-ß-Smads Signaling and Myofibroblast Differentiation by Wei, Yangxuan, Tanaka, Megumu, Sakurai, Takayuki, Kamiyoshi, Akiko, Ichikawa-Shindo, Yuka, Kawate, Hisaka, Cui, Nanqi, Kakihara, Shinji, Zhao, Yunlu, Aruga, Kohsuke, Sanjo, Hideki, Shindo, Takayuki

“…Pulmonary fibrosis is an irreversible, potentially fatal disease. Adrenomedullin (AM) is a multifunctional peptide whose activity is regulated by receptor…”
Get full text

Direct Injection of CRISPR/Cas9-Related mRNA into Cytoplasm of Parthenogenetically Activated Porcine Oocytes Causes Frequent Mosaicism for Indel Mutations by Sato, Masahiro, Koriyama, Miyu, Watanabe, Satoshi, Ohtsuka, Masato, Sakurai, Takayuki, Inada, Emi, Saitoh, Issei, Nakamura, Shingo, Miyoshi, Kazuchika

“…Some reports demonstrated successful genome editing in pigs by one-step zygote microinjection of mRNA of CRISPR/Cas9-related components. Given the relatively…”
Get full text

Author Correction: An Myh11 single lysine deletion causes aortic dissection by reducing aortic structural integrity and contractility by Negishi, Keita, Aizawa, Kenichi, Shindo, Takayuki, Suzuki, Toru, Sakurai, Takayuki, Saito, Yuichiro, Miyakawa, Takuya, Tanokura, Masaru, Kataoka, Yosky, Maeda, Mitsuyo, Tomida, Shota, Morita, Hiroyuki, Takeda, Norifumi, Komuro, Issei, Kario, Kazuomi, Nagai, Ryozo, Imai, Yasushi

Get full text

Transplacental delivery of genome editing components causes mutations in embryonic cardiomyocytes of mid‐gestational murine fetuses by Nakamura, Shingo, Ishihara, Masayuki, Ando, Naoko, Watanabe, Satoshi, Sakurai, Takayuki, Sato, Masahiro

“…Genome editing, as exemplified by CRISPR/Cas9, is now recognized as a powerful tool for the engineering of endogenous target genes. It employs only two…”
Get full text

The Combinational Use of CRISPR/Cas9 and Targeted Toxin Technology Enables Efficient Isolation of Bi-Allelic Knockout Non-Human Mammalian Clones by Watanabe, Satoshi, Sakurai, Takayuki, Nakamura, Shingo, Miyoshi, Kazuchika, Sato, Masahiro

“…Recent advances in genome editing systems such as clustered regularly interspaced short palindromic repeats/CRISPR-associated protein-9 nuclease (CRISPR/Cas9)…”
Get full text

Adrenomedullin–Receptor Activity-Modifying Protein 2 System Ameliorates Subretinal Fibrosis by Suppressing Epithelial-Mesenchymal Transition in Age-Related Macular Degeneration by Tanaka, Masaaki, Kakihara, Shinji, Hirabayashi, Kazutaka, Imai, Akira, Toriyama, Yuichi, Iesato, Yasuhiro, Sakurai, Takayuki, Kamiyoshi, Akiko, Ichikawa-Shindo, Yuka, Kawate, Hisaka, Tanaka, Megumu, Cui, Nanqi, Wei, Yangxuan, Zhao, Yunlu, Aruga, Kohsuke, Yamauchi, Akihiro, Murata, Toshinori, Shindo, Takayuki

“…Age-related macular degeneration (AMD) is a leading cause of visual impairment. Anti–vascular endothelial growth factor drugs used to treat AMD carry the risk…”
Get full text

Fluorescent protein tagging of endogenous protein in brain neurons using CRISPR/Cas9-mediated knock-in and in utero electroporation techniques by Uemura, Takeshi, Mori, Takuma, Kurihara, Taiga, Kawase, Shiori, Koike, Rie, Satoga, Michiru, Cao, Xueshan, Li, Xue, Yanagawa, Toru, Sakurai, Takayuki, Shindo, Takayuki, Tabuchi, Katsuhiko

“…Genome editing is a powerful technique for studying gene functions. CRISPR/Cas9-mediated gene knock-in has recently been applied to various cells and…”
Get full text