Generation of uncultivable forms of Lactobacillus plantarum 8R-A3 under solid-state cultivation on wheat bran

This paper shows that in the solid-state cultivation of Lactobacillus plantarum 8R-A3 on wheat bran, a biofilm was formed on the surface of the carrier within 48 h. Prolongation of fermentation caused a drop in the number of CFU from 96% of the initial total number of cells to 8.8% by 72 h. When the...

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Bibliographic Details
Published in:	Biology bulletin of the Russian Academy of Sciences Vol. 39; no. 6; pp. 525 - 533
Main Authors:	Ushakova, N. A., Abramov, V. M., Khlebnikov, V. S., Semenov, A. M., Kuznetsov, B. B., Kozlova, A. A., Nifatov, A. V., Sakulin, V. K., Kosarev, I. V., Vasilenko, R. N., Sukhacheva, M. V., Mel’nikov, V. G.
Format:	Journal Article
Language:	English
Published:	Dordrecht SP MAIK Nauka/Interperiodica 01-11-2012
Subjects:	Biochemistry Biomedical and Life Sciences Cell Biology Ecology Life Sciences Microbiology Zoology Bran Wheat Bran Solid State Fermentation Biology Bulletin Lactobacillus
Online Access:	Get full text
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Summary:	This paper shows that in the solid-state cultivation of Lactobacillus plantarum 8R-A3 on wheat bran, a biofilm was formed on the surface of the carrier within 48 h. Prolongation of fermentation caused a drop in the number of CFU from 96% of the initial total number of cells to 8.8% by 72 h. When the temperature was raised from 37 to 45°C, which led to drying of the fermentation mass, the CFU index decreased to <10 4 . According to fluorescence microscopy data, up to 40% of bacteria with signs of life survived in the dry specimens. After keeping the mice on a diet with the introduction of 0.05% of fermented bran dried for 72 hours, a strain genetically identical to the L. plantarum 8R-A3 was extracted from the colon. In animals with amikacin-inhibited intestinal lactobacilli, their normal level recovered. It is suggested that L. plantarum 8R-A3 generates uncultivable forms, which are reanimated by passage through the animal organism and exhibit probiotic activity. The biofilm formed in the solid-state cultivation contributes to the survival of lactobacilli cells in drying of the fermentation mass.
ISSN:	1062-3590 1608-3059
DOI:	10.1134/S1062359012050159