Reversible Photocontrol of Microtubule Stability by Spiropyran‐Conjugated Tau‐Derived Peptides

Reversible Photocontrol of Microtubule Stability by Spiropyran‐Conjugated Tau‐Derived Peptides

Spatiotemporal modulation of microtubules by light has become an important aspect of the biological and nanotechnological applications of microtubules. We previously developed a Tau‐derived peptide as a binding unit to the inside of microtubules. Here, we conjugated the Tau‐derived peptide to spirop...

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Bibliographic Details
Published in:Chembiochem : a European journal of chemical biology Vol. 24; no. 8; pp. e202200782 - n/a
Main Authors: Inaba, Hiroshi, Sakaguchi, Minamo, Watari, Soei, Ogawa, Shigesaburo, Kabir, Arif Md. Rashedul, Kakugo, Akira, Sada, Kazuki, Matsuura, Kazunori
Format: Journal Article
Language:English
Published: Germany Wiley Subscription Services, Inc 17-04-2023
Subjects:
Benzopyrans - chemistry
Binding
Irradiation
Microtubules
Modulation
Peptides
Peptides - chemistry
photoisomerization
spiropyran
Spiropyrans
Stability
supramolecular chemistry
tau Proteins - metabolism
Tau-derived peptide
Tubulin
Tubulin - metabolism
Tau-derived peptide
spiropyran
photoisomerization
microtubules
supramolecular chemistry
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Summary:Spatiotemporal modulation of microtubules by light has become an important aspect of the biological and nanotechnological applications of microtubules. We previously developed a Tau‐derived peptide as a binding unit to the inside of microtubules. Here, we conjugated the Tau‐derived peptide to spiropyran, which is reversibly converted to merocyanine by light, as a reversible photocontrol system to stabilize microtubules. Among the synthesized peptides with spiropyran/merocyanine at different positions, several peptides were bound to the inside of microtubules and stabilized the structures of microtubules. The peptide with spiropyran at the N‐terminus induced polymerization and stabilization of microtubules, whereas the same peptide with the merocyanine form did not exert these effects. Reversible formation of microtubules/tubulin aggregates was achieved using the peptide with spiropyran conjugated at the N‐terminus and irradiation with UV and visible light. Spiropyran‐conjugated Tau‐derived peptides would be useful for spatiotemporal modulation of microtubule stability through reversible photocontrol of binding. A system to photocontrol stability of microtubules was developed by conjugating spiropyran to a Tau‐derived peptide, a binding unit to the inside of microtubules. One peptide with spiropyran at the N‐terminus stabilized microtubules, whereas the same peptide with the merocyanine form did not exert the effect. Reversible structural change of microtubules was achieved by photoisomerization of the peptide by irradiation of UV and visible light.
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ISSN:1439-4227
1439-7633
DOI:10.1002/cbic.202200782