Comparative Targeted Metabolomics of Ischemic Stroke: Thrombi and Serum Profiling for the Identification of Stroke-Related Metabolites

Comparative Targeted Metabolomics of Ischemic Stroke: Thrombi and Serum Profiling for the Identification of Stroke-Related Metabolites

Ischemic stroke is one of the leading causes of death and permanent disability in the world. Rapid diagnosis and intervention are crucial for reducing its consequences on individuals and societies. Therefore, identifying reliable biomarkers for early detection, prognostics, and therapy can facilitat...

Full description

Saved in:
Bibliographic Details
Published in:Biomedicines Vol. 12; no. 8; p. 1731
Main Authors: Karmelić, Ivana, Rubić, Ivana, Starčević, Katarina, Ozretić, David, Poljaković, Zdravka, Sajko, Mia Jurilj, Kalousek, Vladimir, Kalanj, Rafaela, Rešetar Maslov, Dina, Kuleš, Josipa, Roje Bedeković, Marina, Sajko, Tomislav, Rotim, Krešimir, Mrljak, Vladimir, Fabris, Dragana
Format: Journal Article
Language:English
Published: Switzerland MDPI AG 02-08-2024
MDPI
Subjects:
Amino acids
Biomarkers
Blood clots
Body mass index
Brain research
Care and treatment
Cholesterol
Health aspects
High density lipoprotein
Hospitals
Ischemia
ischemic stroke
Lipid metabolism
Lipids
Mass spectrometry
metabolic biomarkers
Metabolism
Metabolites
Metabolomics
Patient outcomes
Patients
Phospholipids
Scientific imaging
Software
Stroke
Stroke (Disease)
stroke thrombi
targeted metabolomics
Thrombosis
Triglycerides
Veins & arteries
Croatia
Germany
targeted metabolomics
stroke thrombi
metabolic biomarkers
ischemic stroke
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Ischemic stroke is one of the leading causes of death and permanent disability in the world. Rapid diagnosis and intervention are crucial for reducing its consequences on individuals and societies. Therefore, identifying reliable biomarkers for early detection, prognostics, and therapy can facilitate the early prediction and prevention of stroke. Metabolomics has been shown as a promising tool for biomarker discovery since many post-ischemic metabolites can be found in the plasma or serum of the patient. In this research, we performed a comparative targeted metabolomic analysis of stroke thrombi, stroke patient serums, and healthy control serums in order to determine the alteration in the patients' metabolomes, which might serve as biomarkers for early prediction or stroke prevention. The most statistically altered metabolites characterized in the patient serums compared with the control serums were glutamate and serotonin, followed by phospholipids and triacylglycerols. In stroke thrombi compared with the patients' serums, the most significantly altered metabolites were classified as lipids, with choline-containing phospholipids and sphingomyelins having the highest discriminatory score. The results of this preliminary study could help in understanding the roles of different metabolic changes that occur during thrombosis and cerebral ischemia and possibly suggest new metabolic biomarkers for ischemic stroke.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
These authors contributed equally to this work.
ISSN:2227-9059
2227-9059
DOI:10.3390/biomedicines12081731