T-lymphocytes are not involved in the preventive effect of Fasciola hepatica EVs in DSS-induced acute ulcerative colitis

T-lymphocytes are not involved in the preventive effect of Fasciola hepatica EVs in DSS-induced acute ulcerative colitis

Background: The complexity of the pathogenesis of inflammatory bowel disease has led to the quest of empirically drug therapies, combining immunosuppressant agents, biological therapy and modulators of the microbiota. Helminth parasites have been proposed as an alternative treatment of these disease...

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Bibliographic Details
Published in:Journal of extracellular vesicles Vol. 7; pp. 35 - 36
Main Authors: Galiano, Alicia, Roig, Javier, Sainz, Maria Laura, Trelis, Maria, Cantalapiedra, Fernando, Monteagudo, Carlos, Giner, Elisa, Giner, Rosa M, Recio, M Carmen, Bernal, Dolores, Sánchez-Madrid, Francisco, Marcilla, Antonio
Format: Journal Article
Language:English
Published: Abingdon John Wiley & Sons, Inc 01-01-2018
Subjects:
Colon
Cyclooxygenase-2
Dextran
Disease
Enzyme-linked immunosorbent assay
Fasciola hepatica
Immune response
Immunization
Immunoblotting
Immunomodulation
Inflammatory bowel disease
Inflammatory bowel diseases
Interleukin 6
Intestine
Lymphocytes B
Lymphocytes T
MAP kinase
Microbiota
Mucosa
NF-κB protein
Parasites
Preservation
RAG1 protein
Sodium
Tumor necrosis factor-α
Ulcerative colitis
Vesicles
Spain
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Summary:Background: The complexity of the pathogenesis of inflammatory bowel disease has led to the quest of empirically drug therapies, combining immunosuppressant agents, biological therapy and modulators of the microbiota. Helminth parasites have been proposed as an alternative treatment of these diseases based on the hygiene hypothesis, but ethical and medical problems arise. The identification of extracellular vesicles on those secreted products opens a new field of investigation, since they exert potent immunomodulating effects. Methods: Adult parasites were cultured in vitro and secreted extracellular vesicles were purified and used for immunizing both wild-type C57BL/6 and RAG1-/- mice. Control and immunized mice groups were treated with dextran sulphate sodium 7 days after last immunization to promote experimental colitis. The severity of colitis was assessed by disease activity index and histopathological scores. Mucosal cytokine expression was evaluated by ELISA. The activation of NF-kB, COX-2 and MAPK was evaluated by immunoblotting. Results: Injection of extracellular vesicles from F. hepatica (FhEVs) ameliorated the pathological symptoms induced by DSS in C57BL/6 mice measured by disease activity index, altering pro-inflammatory molecules in the intestine (TNF-α, IL-6 and IL-17A), and interfering with both MAPK and NF-kB pathways. RAG1-/- mice treated with FhEVs showed preservation of tissue architecture whereas colitic mice displayed large disruption areas of the colonic architecture. Summary/conclusion: Our results indicate that extracellular vesicles from parasitic helminths can modulate immune responses in DSSinduced colitis, exerting a protective effect that should be mediated by other cells distinct from B- and T-lymphocytes.
ISSN:2001-3078