RFLP and microsatellite mapping of gene for soybean mosaic virus resistance

Restriction fragment length polymorphisms (RFLPs) and Microsatellites or simple sequence repeats (SSRs) were used as genetic markers to identify the chromosomal location of Rsv, a gene conferring resistance to soybean mosaic virus (SMV). An population was constructed from a cross between soybean lin...

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Bibliographic Details
Published in:	Phytopathology Vol. 84; no. 1; pp. 60 - 64
Main Authors:	Yu, Y.G, Sahgai, M.A, Saghai Maroof, M.A, Buss, G.R, Maughan, P.J, Tolin, S.A
Format:	Journal Article
Language:	English
Published:	St. Paul, MN American Phytopathological Society 01-01-1994
Subjects:	Agronomy. Soil science and plant productions Biological and medical sciences Classical and quantitative genetics. Population genetics. Molecular genetics Fundamental and applied biological sciences. Psychology GENE Generalities. Genetics. Plant material GENES GENETICA Genetics and breeding of economic plants GENETIQUE GLYCINE MAX LIGNEE CONSANGUINE LINEAS CONSANGUINEAS MARCADORES GENETICOS MARQUEUR GENETIQUE Molecular genetics POLIMORFISMO POLYMORPHISME POTYVIRUS DEL MOSAICO DE LA SOJA POTYVIRUS MOSAIQUE SOJA RESISTANCE AUX MALADIES RESISTENCIA A LA ENFERMEDAD SECUENCIA NUCLEICA SEQUENCE NUCLEIQUE VARIEDADES VARIETE Plant pathogen Genetic marker Microorganism resistance Gene Dicotyledones Angiospermae Genetics Molecular probe Potyvirus Nucleic acid Genetic mapping Soybean mosaic virus Glycine max Oil plant(vegetal) Virus Grain legume Sensitivity resistance Restriction fragment length polymorphism Leguminosae Plant pathogen resistance DNA Plant origin Spermatophyta Molecular biology Genome
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Summary:	Restriction fragment length polymorphisms (RFLPs) and Microsatellites or simple sequence repeats (SSRs) were used as genetic markers to identify the chromosomal location of Rsv, a gene conferring resistance to soybean mosaic virus (SMV). An population was constructed from a cross between soybean line PI 96983 as the resistant parent and cultivar Lee 68 as the susceptible parent. Twenty-five RFLP and three SSR loci, polymorphic between the parental lines, were analyzed in 107 F2 individuals. Genotypes of Rsv were determined by inoculating F2:3 progeny with the G1 strain of SMV. Data also were collected for an additional soybean gene (w1/W1), which controls anthocyanin pigmentation in hypocotyls and flowers. Analyses of the data revealed that the SSR marker, SM176 (a soybean heat shock protein gene), and two RFLP markers, pA186 and pK644a, are closely linked to Rsv, with distances of 0.5, 1.5, and 2.1 centiMorgans, respectively. The close links between Rsv and the three markers were confirmed by marker analysis of three Williams near-isogenic lines (NILs) that carry Rsv alleles from PI 96983 and Marshall. Marker analysis also indicated that the SMV resistance gene in Buffalo is probably at the Rsv locus
Bibliography:	9500207 F30 H20
ISSN:	0031-949X 1943-7684
DOI:	10.1094/phyto-84-60