Single-Cell RNA Sequencing of Arabidopsis Leaf Tissues Identifies Multiple Specialized Cell Types: Idioblast Myrosin Cells and Potential Glucosinolate-Producing Cells

The glucosinolate-myrosinase defense system (GMDS), characteristic of Brassicales, is involved in plant defense. Previous single-cell transcriptomic analyses have reported the expression profiles of multiple GMDS-related cell types (i.e. myrosinase-rich myrosin idioblasts and multiple types of poten...

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Bibliographic Details
Published in:	Plant and cell physiology Vol. 64; no. 2; pp. 234 - 247
Main Authors:	Maeda, Taro, Sugano, Shigeo S, Shirakawa, Makoto, Sagara, Mayu, Ito, Toshiro, Kondo, Satoshi, Nagano, Atsushi J
Format:	Journal Article
Language:	English
Published:	Japan 01-03-2023
Subjects:	Arabidopsis - genetics Glucosinolates - metabolism Glucuronidase - metabolism Glycoside Hydrolases - metabolism Plant Leaves - metabolism Plants - metabolism Sequence Analysis, RNA Myrosin cell Glucosinolate–myrosinase complexes scRNA-Seq Arabidopsis S-cell WRKY23
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Summary:	The glucosinolate-myrosinase defense system (GMDS), characteristic of Brassicales, is involved in plant defense. Previous single-cell transcriptomic analyses have reported the expression profiles of multiple GMDS-related cell types (i.e. myrosinase-rich myrosin idioblasts and multiple types of potential glucosinolate synthetic cells as well as a candidate S-cell for glucosinolate accumulation). However, differences in plant stages and cell-type annotation methods have hindered comparisons among studies. Here, we used the single-cell transcriptome profiles of extended Arabidopsis leaves and verified the distribution of previously used markers to refine the expression profiles of GMDS-associated cell types. Moreover, we performed beta-glucuronidase promoter assays to confirm the histological expression patterns of newly obtained markers for GMDS-associated candidates. As a result, we found a set of new specific reporters for myrosin cells and potential glucosinolate-producing cells.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0032-0781 1471-9053
DOI:	10.1093/pcp/pcac167