Search Results - "Sachdev, Shaurya"
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Molecular Processes Leading to “Necking” in Extensional Flow of Polymer Solutions: Using Microfluidics and Single DNA Imaging
Published in Macromolecules (27-12-2016)“…We study the necking and pinch-off dynamics of liquid droplets that contain a semidilute polymer solution of polyacrylamide close to overlap concentration by…”
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Efficient Gene Transfection by Electroporation—In Vitro and In Silico Study of Pulse Parameters
Published in Applied sciences (01-08-2022)“…Gene electrotransfer (GET) is a widely used method for nucleic acids’ delivery into cells. We explored, evaluated, and demonstrated the potential use of…”
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Gene Electrotransfer into Mammalian Cells Using Commercial Cell Culture Inserts with Porous Substrate
Published in Pharmaceutics (01-09-2022)“…Gene electrotransfer is one of the main non-viral methods for intracellular delivery of plasmid DNA, wherein pulsed electric fields are used to transiently…”
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Revisiting the role of pulsed electric fields in overcoming the barriers to in vivo gene electrotransfer
Published in Bioelectrochemistry (Amsterdam, Netherlands) (01-04-2022)“…[Display omitted] Gene therapies are revolutionizing medicine by providing a way to cure hitherto incurable diseases. The scientific and technological advances…”
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DNA-membrane complex formation during electroporation is DNA size-dependent
Published in Biochimica et biophysica acta. Biomembranes (01-02-2020)“…Size of DNA molecules governs their interaction with the cell membrane during electroporation and their subsequent transport inside the cell. In order to…”
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Bridging the scales in high-throughput dielectrophoretic (bio-)particle separation in porous media
Published in Scientific reports (11-07-2018)“…Dielectrophoresis (DEP) is a versatile technique for the solution of difficult (bio-)particle separation tasks based on size and material. Particle motion by…”
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DNA translocation to giant unilamellar vesicles during electroporation is independent of DNA size
Published in Soft matter (2019)“…Delivery of naked DNA molecules into living cells via physical disruption of the membrane under electric pulses has potential biomedical applications ranging…”
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