Stryphnodendron adstringens (“Barbatimão”) Leaf Fraction: Chemical Characterization, Antioxidant Activity, and Cytotoxicity Towards Human Breast Cancer Cell Lines

We evaluated the chemical composition, antioxidant activity, and antitumor potential of a fraction that was isolated from Stryphnodendron adstringens (barbatimão) leaf aqueous extract. Fraction is composed by gallic acid, procyanidin dimer B1, and (−)-epicatechin-3- O -gallate and it exhibits antiox...

Full description

Saved in:

Bibliographic Details
Published in:	Applied biochemistry and biotechnology Vol. 184; no. 4; pp. 1375 - 1389
Main Authors:	Sabino, A. P. L., Eustáquio, L. M. S., Miranda, A. C. F., Biojone, C., Mariosa, T. N., Gouvêa, Cibele Marli Cação Paiva
Format:	Journal Article
Language:	English
Published:	New York Springer US 01-04-2018 Springer Nature B.V
Subjects:	Anticancer properties Antineoplastic Agents, Phytogenic - chemistry Antineoplastic Agents, Phytogenic - pharmacology Antioxidants Antioxidants - chemistry Antioxidants - pharmacology Apoptosis Bcl-2 protein Biochemistry Biotechnology Breast cancer Breast Neoplasms - drug therapy Breast Neoplasms - metabolism Breast Neoplasms - pathology Cancer Caspase Caspase-3 Caspase-8 Caspase-9 Cell death Chemistry Chemistry and Materials Science Cytotoxicity Cytotoxins - chemistry Cytotoxins - pharmacology Drug development Epicatechin Fabaceae - chemistry Female Gallic acid Humans Leaves MCF-7 Cells Neoplasm Proteins - metabolism Plant Extracts - chemistry Plant Extracts - pharmacology Plant Leaves - chemistry Rounding Shrinkage Stryphnodendron adstringens Sulforhodamine Toxicity Tumor cell lines Western blot MDA-MB-435 Cell death MCF-7 DNA fragmentation Plant extract Anticancer
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	We evaluated the chemical composition, antioxidant activity, and antitumor potential of a fraction that was isolated from Stryphnodendron adstringens (barbatimão) leaf aqueous extract. Fraction is composed by gallic acid, procyanidin dimer B1, and (−)-epicatechin-3- O -gallate and it exhibits antioxidant and cytotoxic activities. Fraction was cytotoxic against two human breast cancer cell lines, ER (+) and MCF-7 and the triple-negative, MDA-MB-435. The sulforhodamine B assay showed that, as compared to normal control cells, the fraction significantly ( P < 0.05) decreased cancer cell viability. The morphological alterations noted in the treated cancer cells were cell rounding-up, shrinkage, and nuclear condensation reduction of cell diameter and length. Treatment with fraction increased cancer cell expression of Bax, caspase-9, active caspase-3, caspase-8, LC-3, and beclin-1 and decreased Bcl-2, caspase-3, and pro-caspase-8 expression. Altogether, fraction is cytotoxic to both breast cancer cell lines, induces cell death, and its mechanism of action seems to include the induction of apoptosis. Our data support a positive role of the fraction as a chemopreventive agent for antineoplastic drug development.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0273-2289 1559-0291
DOI:	10.1007/s12010-017-2632-z