Mutants of Streptococcus lactis subsp. diacetylactis lacking diacetyl reductase activity

Three strains of Streptococcus lactis subsp. diacetylactis, namely DRC-1, DRC-2 and DRC-3 which produced diacetyl up to 120 h of incubation were exposed to the ultraviolet irradiation as well as N-methyl-N'-nitro-N-nitrosoguanidine (NTG) to isolate mutants lacking diacetyl reductase activity. U...

Full description

Saved in:

Bibliographic Details
Published in:	Acta microbiologica Polonica Vol. 27; no. 4; p. 353
Main Authors:	Arora, B C, Dutta, S M, Sabharwal, V B, Ranganathan, B
Format:	Journal Article
Language:	English
Published:	Poland 1978
Subjects:	Acetoin Dehydrogenase - genetics Acetoin Dehydrogenase - metabolism Alcohol Oxidoreductases - genetics Cell-Free System Diacetyl - metabolism Lactococcus lactis - drug effects Lactococcus lactis - genetics Lactococcus lactis - radiation effects Methylnitronitrosoguanidine - pharmacology Mutation Ultraviolet Rays
Online Access:	Get more information
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Three strains of Streptococcus lactis subsp. diacetylactis, namely DRC-1, DRC-2 and DRC-3 which produced diacetyl up to 120 h of incubation were exposed to the ultraviolet irradiation as well as N-methyl-N'-nitro-N-nitrosoguanidine (NTG) to isolate mutants lacking diacetyl reductase activity. UV irradiation did not produce any isolate completely devoid of diacetyl reductase activity, though, 99.5% loss in activity could be achieved. NTG treatment proved to be more effective and seven survivors exhibiting complete loss of diacetyl reductase activity were recovered. These altered characteristics were retained on repeated subculturing.
ISSN:	0137-1320