Morphological aspects of small intestinal mucosal injury and repair after electron irradiation

Morphological evaluation of the small intestine mucosa and apoptosis activity (caspase-3) is necessary to assess the severity of damage to the small intestine. At the same time, proliferative index based on Ki-67 can be used to assess the regenerative potential of the small intestine. Fragments of s...

Full description

Saved in:

Bibliographic Details
Published in:	Anatomy & cell biology Vol. 57; no. 3; pp. 384 - 391
Main Authors:	Demyashkin, Grigory, Karakaeva, Elza, Saakian, Siuzanna, Shchekin, Vladimir, Elbuzdukaev, Emir, Bamatgiraev, Umar, Ashgalieva, Daniia, Evsultanova, Makka, Kovalev, Daniil, Kabanova, Darya, Shatunov, Oleg, Atiakshin, Dmitrii
Format:	Journal Article
Language:	English
Published:	Korea (South) Korean Association of Anatomists 30-09-2024 대한해부학회
Subjects:	Original 해부학 Proliferation Villus-crypt Electron irradiation Small intestinal Apoptosis
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Morphological evaluation of the small intestine mucosa and apoptosis activity (caspase-3) is necessary to assess the severity of damage to the small intestine. At the same time, proliferative index based on Ki-67 can be used to assess the regenerative potential of the small intestine. Fragments of small intestine of Wistar rats (n=60) of three groups: I) control (n=20); II) experimental group (n=20; local single electron irradiation at a dose of 2 Gy), III) experimental group (n=20; local single electron irradiation at a dose of 8 Gy) were studied by light microscopy using hematoxylin and eosin staining and immunohistochemical reactions with antibodies to Ki-67 and caspase-3. In all samples of the experimental groups, a decrease in all morphometric indices was observed on day 1 with a tendency to recover on day 3. Small intestinal electron irradiation led to disturbances in the histoarchitecture of varying severity, and an increase in cell apoptosis was observed (increased expression of caspase-3 and decrease in Ki-67). In addition, modulation of the PI3K/AKT and MAPK/ERK signaling pathways was detected. The most pronounced destructive changes were observed in the group of 8 Gy single electron irradiation. Local irradiation of the small intestine with electrons at a dose of 2 and 8 Gy results in a decrease in the number of enterocytes, mainly stem cells of the intestinal crypts.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	2093-3665 2093-3673
DOI:	10.5115/acb.24.050