Production of Biological De-Emulsification by Bacterial Isolates Isolated from Local Soils
The purpose of this research was to isolate local bacterial have the effectiveness of de-emulsification in liquid emulsion. Twenty local isolates of actinomycetes were isolated from soil contaminated with oil waste using mineral liquid medium at initial pH of 7 in the presence of oil waste (1%) as a...
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| Published in: | Engineering and Technology Journal Vol. 32; no. 6B; pp. 80 - 85 |
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| Main Authors: | , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
Unviversity of Technology- Iraq
01-06-2014
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| Subjects: | |
| Online Access: | Get full text |
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| Summary: | The purpose of this research was to isolate local bacterial have the effectiveness of de-emulsification in liquid emulsion. Twenty local isolates of actinomycetes were isolated from soil contaminated with oil waste using mineral liquid medium at initial pH of 7 in the presence of oil waste (1%) as a source of carbon and incubated at 40 °C for 5 days . The primary screening of isolates was tested to de-emulsifying ability.Results indicate that the isolates ST3 , ST8 and ST15 were selected for theirhigh ability to de-emulsification. Secondary screening of selected isolates was estimated to deemulsification using the spore suspension . Results indicate that the ST8 isolate showed the highest de-emulsification activity, subsequently was identified and designated as Nocardia sp.. Studied the effects of both the spores number and period of exposure on the effectiveness of de-emulsification. The results showed that the 0.5 ml of spore suspension (107 spore/ml) gave the best effective way to de-emulsifying, while 30 min. the best period appropriate to de-emulsifying. |
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| ISSN: | 1681-6900 2412-0758 |
| DOI: | 10.30684/etj.32.6B.20 |