Search Results - "Sánchez, Aniel"
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Mass spectrometric and kinetics characterization of modified species of Growth Hormone Releasing Hexapeptide generated under thermal stress in different pH and buffers
Published in Journal of pharmaceutical and biomedical analysis (05-02-2021)“…[Display omitted] •pH and thermal stress study (pH 3.0–8.0, 60 °C, 35 days) of GHRP-6 is presented.•Hydrolysis, cyclization and acylation as the main…”
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2
Proteomic Analysis of Honey Bee Brain upon Ontogenetic and Behavioral Development
Published in Journal of proteome research (06-03-2009)“…The honey bee (Apis mellifera) is a social insect that shows complex and integrated behaviors. Its ability to read and respond to several sets of extrinsic and…”
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3
Mass spectrometry evaluation of a neuroblastoma SH-SY5Y cell culture protocol
Published in Analytical biochemistry (15-10-2018)“…Cell line-based proteomics studies are susceptible to intrinsic biological variation that contributes to increasing false positive claims; most of the methods…”
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Quantitative proteomic analysis identifies proteins and pathways related to neuronal development in differentiated SH-SY5Y neuroblastoma cells
Published in EuPA open proteomics (01-09-2017)“…[Display omitted] •Differentiation analysis of SH-SY5Y cells with iTRAQ strategy is proposed.•Differentiated SH-SY5Y cells are more appropriated as a neuronal…”
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Sodium dodecyl sulfate free gel electrophoresis/electroelution sorting for peptide fractionation
Published in Journal of separation science (01-12-2019)“…Shotgun proteomics based on peptide fractionation by using liquid chromatography has become the common procedure for proteomic studies, although in the very…”
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Characterization of low‐abundance species in the active pharmaceutical ingredient of CIGB‐300: A clinical‐grade anticancer synthetic peptide
Published in Journal of peptide science (01-06-2018)“…CIGB‐300 is a first‐in‐class synthetic peptide‐based drug of 25 amino acids currently undergoing clinical trials in cancer patients. It contains an amidated…”
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Targeting the hydrophilic regions of recombinant proteins by MS via in-solution buffer-free trypsin digestion
Published in European journal of mass spectrometry (Chichester, England) (01-06-2020)“…A desalting step using reversed phase chromatography is a common practice prior to mass spectrometry analysis of proteolytic digests in spite of the…”
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Periplasmic proteins encoded by VCA0261―0260 and VC2216 genes together with copA and cueR products are required for copper tolerance but not for virulence in Vibrio cholerae
Published in Microbiology (Society for General Microbiology) (01-08-2012)“…The bacterial pathogen Vibrio cholerae requires colonizination of the human small intestine to cause cholera. The anaerobic and slightly acidic conditions…”
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HI-Bone: A Scoring System for Identifying Phenylisothiocyanate-Derivatized Peptides Based on Precursor Mass and High Intensity Fragment Ions
Published in Analytical chemistry (Washington) (02-04-2013)“…Peptide sequence matching algorithms used for peptide identification by tandem mass spectrometry (MS/MS) enumerate theoretical peptides from the database,…”
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Development and validation of a bioanalytical LC–MS method for the quantification of GHRP-6 in human plasma
Published in Journal of pharmaceutical and biomedical analysis (23-02-2012)“…► A quantitative method for GHRP-6 in human plasma was developed and fully validated. ► 13C-labeled Alanine GHRP-6 as internal standard allowed relative…”
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Effectively addressing complex proteomic search spaces with peptide spectrum matching
Published in Bioinformatics (15-05-2013)“…Protein identification by mass spectrometry is commonly accomplished using a peptide sequence matching search algorithm, whose sensitivity varies inversely…”
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In silico analysis of accurate proteomics, complemented by selective isolation of peptides
Published in Journal of proteomics (06-09-2011)“…Protein identification by mass spectrometry is mainly based on MS/MS spectra and the accuracy of molecular mass determination. However, the high complexity and…”
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Peptide fractionation by acid pH SDS-free electrophoresis
Published in Electrophoresis (01-06-2011)“…SDS‐free polyacrylamide gel electrophoresis is an effective alternative approach to peptide fractionation. Here we describe a discontinuous buffer system at…”
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Proteomics Based on Peptide Fractionation by SDS-Free PAGE
Published in Journal of proteome research (01-06-2008)“…Here we demonstrate the usefulness of peptide fractionation by SDS-free polyacrylamide gel electrophoresis and its applicability to proteomics studies. In the…”
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15
Recombinant truncated tilapia growth hormone enhances growth and innate immunity in tilapia fry ( Oreochromis sp.)
Published in General and comparative endocrinology (15-05-2008)“…Abstract Pichia pastoris cells transformed with a plasmid engineered for the expression of tilapia growth hormone as a secreted product produced a…”
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Introducing an Asp-Pro Linker in the Synthesis of Random One-Bead-One-Compound Hexapeptide Libraries Compatible with ESI-MS Analysis
Published in ACS combinatorial science (12-03-2012)“…A random hexapeptide library (one-bead-one-compound), containing sixteen amino acids (166 different sequences) was synthesized on a Tentagel resin previously…”
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PRIDE Inspector Toolsuite: Moving Toward a Universal Visualization Tool for Proteomics Data Standard Formats and Quality Assessment of ProteomeXchange Datasets
Published in Molecular & cellular proteomics (01-01-2016)“…The original PRIDE Inspector tool was developed as an open source standalone tool to enable the visualization and validation of mass-spectrometry (MS)-based…”
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Comparative proteomic analysis of growth hormone secretagogue A233 treatment of murine macrophage cells J774A.2 indicates it has a role in antiviral innate response
Published in Biochemistry and biophysics reports (01-03-2016)“…Growth hormone secretagogues (GHS), among other factors, regulate the release of GH. The biological activity of the secretagogue peptide A233 as a promoter of…”
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Anaerobic growth promotes synthesis of colonization factors encoded at the Vibrio pathogenicity island in Vibrio cholerae El Tor
Published in Research in microbiology (01-01-2009)“…Pathogenesis of the facultative anaerobe Vibrio cholerae takes place at the gut under low oxygen concentrations. To identify proteins which change their…”
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Anti-HIV-1 and anti-HBV immune responses in mice after parenteral and nasal co-administration of a multiantigenic formulation
Published in Current HIV research (01-09-2008)“…The cell-mediated immune response to HIV-1 is an essential element of the mechanisms for viral replication control. Currently, most of the vaccine candidates…”
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