Expression of human adenosine deaminase in nonhuman primates after retrovirus-mediated gene transfer

Primate bone marrow cells were infected with a retroviral vector carrying the genes for human adenosine deaminase (h-ADA) and bacterial neomycin resistance (neor). The infected cells were infused back into the lethally irradiated donor animals. Several monkeys fully reconstituted and were shown to e...

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Bibliographic Details
Published in:	The Journal of experimental medicine Vol. 166; no. 1; pp. 219 - 234
Main Authors:	KANTOFF, P. W, GILLIO, A. P, KARLSSON, S, SWIEBEL, J. A, GILBOA, E, BLAESE, R. M, NIENHUIS, A, O'REILLY, R. J, FRENCH ANDERSON, W, MCLACHLIN, J. R, BORDIGNON, C, EGLITIS, M. A, KERNAN, N. A, MOEN, R. C, KOHN, D. B, SHEAU-FUNG YU, KARSON, E
Format:	Journal Article
Language:	English
Published:	New York, NY Rockefeller University Press 01-07-1987 The Rockefeller University Press
Subjects:	adenosine deaminase Adenosine Deaminase - genetics Animals Biological and medical sciences Bone Marrow Cells Bone Marrow Transplantation DNA - genetics Drug Resistance - genetics Experimental viral diseases and models gene transfer Hematopoietic Stem Cells - enzymology Humans Infectious diseases Lymphocytes - enzymology Macaca fascicularis man Medical sciences Monocytes - enzymology Neomycin Nucleic Acid Hybridization Nucleoside Deaminases - genetics primates Retroviridae - genetics retrovirus transfection Transformation, Genetic Viral diseases Whole-Body Irradiation Deficiency Monkey Retroviridae Adenosine deaminase Gene expression Genetic transfer Virus Vertebrata Mammalia Gene Bone marrow Primates Models
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Summary:	Primate bone marrow cells were infected with a retroviral vector carrying the genes for human adenosine deaminase (h-ADA) and bacterial neomycin resistance (neor). The infected cells were infused back into the lethally irradiated donor animals. Several monkeys fully reconstituted and were shown to express the h-ADA and neor genes at low levels in their recirculating hematopoietic cells for short periods of time.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0022-1007 1540-9538
DOI:	10.1084/jem.166.1.219