Monitoring of microbial souring in chemically treated, produced-water biofilm systems using molecular techniques

The identification of bacteria in oil production facilities has previously been based on culture techniques. However, cultivation of bacteria from these often-extreme environments can lead to errors in identifying the microbial community members. In this study, molecular techniques including fluores...

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Bibliographic Details
Published in:	Journal of industrial microbiology & biotechnology Vol. 32; no. 4; pp. 163 - 170
Main Authors:	KJELLERUP, B. V, VEEH, R. H, SUMITHRARATNE, P, THOMSEN, T. R, BUCKINGHAM-MEYER, K, FRØLUND, B, STURMAN, P
Format:	Journal Article
Language:	English
Published:	Heidelberg Springer 01-04-2005 Oxford University Press
Subjects:	Bacteria Biofilms Biofilms - growth & development Biological and medical sciences Bioreactors Biotechnology Chemical treatment Culture Media Culture techniques Electrophoresis - methods Fuel Oils Fundamental and applied biological sciences. Psychology In Situ Hybridization, Fluorescence Industrial Waste Microbiology Molecular Sequence Data Molybdenum - metabolism Molybdenum - pharmacology Nitrates Nitrates - metabolism Nitrates - pharmacology Nitrites - metabolism Nitrites - pharmacology Petroleum production Phylogeny Polymerase Chain Reaction Sequence Analysis, DNA Sulfate reduction Sulfates Sulfides Sulfides - metabolism Sulfur-Reducing Bacteria - classification Sulfur-Reducing Bacteria - genetics Sulfur-Reducing Bacteria - growth & development Sulfur-Reducing Bacteria - isolation & purification Waste Management Water Microbiology Water Pollutants, Chemical Water Polymerase chain reaction Sulfate-reducing bacteria Chemical treatment Fluorescence in situ hybridization Biofilm Denaturing gradient gel electrophoresis Microorganism Technique Fluorescence in situ hybridization ·Polymerase chain reaction ·Denaturing gradient gel electrophoresis Monitoring Oil souring·Chemical treatment
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Summary:	The identification of bacteria in oil production facilities has previously been based on culture techniques. However, cultivation of bacteria from these often-extreme environments can lead to errors in identifying the microbial community members. In this study, molecular techniques including fluorescence in situ hybridization, PCR, denaturing gradient gel electrophoresis, and sequencing were used to track changes in bacterial biofilm populations treated with nitrate, nitrite, or nitrate+molybdate as agents for the control of sulfide production. Results indicated that nitrite and nitrate+molybdate reduced sulfide production, while nitrate alone had no effect on sulfide generation. No long-term effect on sulfide production was observed. Initial sulfate-reducing bacterial numbers were not influenced by the chemical treatments, although a significant increase in sulfate-reducing bacteria was observed after termination of the treatments. Molecular analysis showed a diverse bacterial population, but no major shifts in the population due to treatment effects were observed.
ISSN:	1367-5435 1476-5535
DOI:	10.1007/s10295-005-0222-5