Expression of genes encoding late nodulins characterized by a putative signal peptide and conserved cysteine residues is reduced in ineffective pea nodules

Five nodulin genes, PsN1, PsN6, PsN314, PsN335, and PsN466, with reduced expression in ineffective nodules on the pea (Pisum sativum) mutant E135 (sym13) were characterized. They encode small polypeptides containing a putative signal peptide and conserved cysteine residues and show homology to the n...

Full description

Saved in:

Bibliographic Details
Published in:	Molecular plant-microbe interactions Vol. 15; no. 2; pp. 129 - 137
Main Authors:	KATO, Takashi, KAWASHIMA, Kazuya, MIWA, Masami, MIMURA, Yoshifumi, TAMAOKI, Masanori, KOUCHI, Hiroshi, SUGANUMA, Norlo
Format:	Journal Article
Language:	English
Published:	St Paul, MN APS Press 01-02-2002 The American Phytopathological Society
Subjects:	Agronomy. Soil science and plant productions Amino Acid Sequence Biological and medical sciences Cloning, Molecular Conserved Sequence Cysteine Economic plant physiology Fundamental and applied biological sciences. Psychology Membrane Proteins - genetics Molecular Sequence Data Oxidation-Reduction Parasitism and symbiosis Pisum sativum - genetics Plant physiology and development Plant Proteins - chemistry Plant Proteins - genetics Protein Isoforms - genetics Protein Sorting Signals - genetics Recombinant Proteins - chemistry Sequence Alignment Sequence Homology, Amino Acid Symbiosis Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...) Symbiosis Leader peptide Hemoprotein Symbionte Symbiotic relation Functional deficit Plant nodule Gene expression Spatial variation Structure function relationship Time variation Grain legume Leguminosae Efficiency Dicotyledones Pisum sativum Angiospermae Nodulin Nitrogen fixation Leghemoglobin Bacteria Spermatophyta Rhizobiaceae Intraspecific comparison
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Five nodulin genes, PsN1, PsN6, PsN314, PsN335, and PsN466, with reduced expression in ineffective nodules on the pea (Pisum sativum) mutant E135 (sym13) were characterized. They encode small polypeptides containing a putative signal peptide and conserved cysteine residues and show homology to the nodulins PsENOD3/14 and PsNOD6. For each gene, multiple bands were detected by genomic Southern analysis. Northern analysis showed that all five genes were expressed exclusively in nodules and that their temporal expression patterns were similar to that of the leghemoglobin (Lb) gene during nodule development. Their transcripts were localized predominantly from the interzone II-III to the distal part of nitrogen-fixing zone III in effective nodules, resembling the Lb gene. However, transcripts in ineffective E135 nodules were localized in narrower regions than those in the effective nodules. These results indicate that these nodulins are abundant in pea nodules and that their successive expression during nodule development is associated with nitrogen-fixing activity.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0894-0282 1943-7706
DOI:	10.1094/mpmi.2002.15.2.129