Search Results - "STENGER, D. A"

Large scale screening of CRISPR guide RNAs using an optimized high throughput robotics system by Spangler, J. R., Leski, T. A., Schultzhaus, Z., Wang, Z., Stenger, D. A.

“…All CRISPR/CAS systems utilize CRISPR guide RNAs (crRNAs), the design of which depend on the type of CAS protein, genetic target and the environment/matrix…”
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Machine learning for design of degenerate Cas13a crRNAs using lassa virus as a model of highly variable RNA target by Leski, T. A., Spangler, J. R., Wang, Z., Schultzhaus, Z., Taitt, C. R., Dean, S. N., Stenger, D. A.

“…The design of minimum CRISPR RNA (crRNA) sets for detection of diverse RNA targets using sequence degeneracy has not been systematically addressed. We tested…”
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Development and application of cell-based biosensors by PANCRAZIO, J. J, WHELAN, J. P, BORKHOLDER, D. A, MA, W, STENGER, D. A

“…Biosensors incorporate a biological sensing element that converts a change in an immediate environment to signals conducive for processing. Biosensors have…”
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Spatially Controlled Adhesion, Spreading, and Differentiation of Endothelial Cells on Self-Assembled Molecular Monolayers by Spargo, B. J., Testoff, M. A., Nielsen, T. B., Stenger, D. A., Hickman, J. J., Rudolf, A. S.

“…Chemically modified glass substrates were used to demonstrate differential adhesion, growth, and differentiation of endothelial cells. Endothelial cells were…”
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Surface determinants of neuronal survival and growth on self-assembled monolayers in culture by Stenger, D A, Pike, C J, Hickman, J J, Cotman, C W

“…We have studied the modulation of hippocampal neuron morphological development in vitro using surfaces derivatized with aminosilane self-assembled monolayers…”
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Microlithographic determination of axonal/dendritic polarity in cultured hippocampal neurons by Stenger, David A., Hickman, James J., Bateman, Karen E., Ravenscroft, Melissa S., Ma, Wu, Pancrazio, Joseph J., Shaffer, Kara, Schaffner, Anne E., Cribbs, David H., Cotman, Carl W.

“…High resolution substrates, created using patterned self-assembled monolayers, are shown to direct axonal and dendritic process extension at the level of a…”
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Deep UV Photochemistry of Chemisorbed Monolayers: Patterned Coplanar Molecular Assemblies by Dulcey, Charles S., Georger, Jacque H., Krauthamer, Victor, Stenger, David A., Fare, Thomas L., Calvert, Jeffrey M.

“…Deep ultraviolet (UV) irradiation is shown to modify organosilane self-assembled monolayer (SAM) films by a photocleavage mechanism, which renders the surface…”
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Acute exposure of toluene transiently potentiates p42/44 mitogen-activated protein kinase (MAPK) activity in cultured rat cortical astrocytes by Lin, H.J, Shaffer, K.M, Chang, Y.H, Barker, J.L, Pancrazio, J.J, Stenger, D.A, Ma, W

“…It has been shown that the inhalation of toluene in rats can cause neuronal apoptosis in the central nervous system. However, the cellular and molecular…”
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Quantitative assessment of filter-based cDNA microarrays: gene expression profiles of human T-lymphoma cell lines by Dodson, J.M., Charles, P.T., Stenger, D.A., Pancrazio, J.J.

“…Motivation: While the use of cDNA microarrays for functional genomic analysis has become commonplace, relatively little attention has been placed on false…”
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Surveillance of transcriptomes in basic military trainees with normal, febrile respiratory illness, and convalescent phenotypes by Thach, D C, Agan, B K, Olsen, C, Diao, J, Lin, B, Gomez, J, Jesse, M, Jenkins, M, Rowley, R, Hanson, E, Tibbetts, C, Stenger, D A, Walter, E

“…Gene expression profiles permit analysis of host immune response at the transcriptome level. We used the Pax gene Blood RNA (PAX) System and Affymetrix…”
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Description and demonstration of a CMOS amplifier-based-system with measurement and stimulation capability for bioelectrical signal transduction by Pancrazio, Joseph J., Bey, Jr, Paul P., Loloee, Arash, SubbaRao Manne, Chao, Hui-Chuan, L. Howard, Lorn, Milton Gosney, W., Borkholder, David A., T.A. Kovacs, Gregory, Manos, Patricia, Cuttino, David S., A. Stenger, David

“…An extracellular recording system incorporating an electrode array and an amplifier/stimulator CMOS chip is described and characterized. Important features of…”
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Investigation of the factors necessary for growth of hippocampal neurons in a defined system by Schaffner, Anne E., Barker, Jeffery L., Stenger, David A., Hickman, James J.

“…We have developed an in vitro system that combines the use of a defined medium with a chemically defined surface for the differentiation of embryonic rat…”
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Trimethylolpropane Phosphate Induces Epileptiform Discharges in the CA1 Region of the Rat Hippocampus by Lin, J., Ritchie, G.D., Stenger, D.A., Nordholm, A.F., Pancrazio, J.J., Rossi, J.

“…The actions of trimethylolpropane phosphate (TMPP), an ethyl bicyclophosphate convulsant produced during the partial pyrolysis of some phosphate ester-based…”
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Gene Modulation in Total Brain Induced by Exposure to the Bicyclic Phosphorus Ester Trimethylolpropane Phosphate (TMPP) by Russell, A.C., Bekkedal, M.Y.-V., Mann, T.T., Ritchie, G.D., Rossi, J., Stenger, D.A., Pancrazio, J.J., Andreadis, J.D.

“…The effect of a single subconvulsive dose of the GABAergic convulsant trimethylolpropane phosphate (TMPP) on gene expression in total rat brain was examined…”
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Inhibition of spontaneous GABAergic transmission by trimethylolpropane phosphate by Kao, W Y, Liu, Q Y, Ma, W, Ritchie, G D, Lin, J, Nordholm, A F, Rossi, 3rd, J, Barker, J L, Stenger, D A, Pancrazio, J J

“…Trimethylolpropane phosphate (TMPP) is a neuroactive organophosphate generated during partial pyrolysis of a synthetic ester turbine engine lubricant. While…”
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X-ray and Ellipsometric Studies of Self-Assembled Monolayers of Fluorinated Chlorosilanes by Geer, R. E, Stenger, D. A, Chen, M. S, Calvert, J. M, Shashidhar, R, Jeong, Y. H, Pershan, P. S

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Characterization of rat spinal cord neurons cultured in defined media on microelectrode arrays by Manos, P, Pancrazio, J.J, Coulombe, M.G, Ma, W, Stenger, D.A

“…Previous efforts to utilize mammalian spinal cord neurons as biosensor elements have relied on neuronal: glial co-cultures maintained in serum-containing…”
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Identification of differential gene expression profiles in rat cortical cells exposed to the neuroactive agents trimethylolpropane phosphate and bicuculline by Andreadis, J.D., Mann, T.T., Russell, A.C., Stenger, D.A., Pancrazio, J.J.

“…Recent technological advancements in microfabrication combined with the rapid acquisition of full genome sequence data have led to the development of DNA…”
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Influence of extracellular matrix proteins on membrane potentials and excitability in NG108-15 cells by Kowtha, V.C, Bryant, H.J, Pancrazio, J.J, Stenger, D.A

“…Previous studies have demonstrated that components of the extracellular matrix can induce neurite extension and cell adhesion in the neuroblastoma×glioma…”
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Detection of physiologically active compounds using cell-based biosensors by Stenger, David A., Gross, Guenter W., Keefer, Edward W., Shaffer, Kara M., Andreadis, Joanne D., Ma, Wu, Pancrazio, Joseph J.

“…Cell-based biosensors are portable devices that contain living biological cells that monitor physiological changes induced by exposure to environmental…”
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