Search Results - "STANDING, Kenneth G"

Citrus Chlorophyllase Dynamics at Ethylene-Induced Fruit Color-Break: A Study of Chlorophyllase Expression, Posttranslational Processing Kinetics, and in Situ Intracellular Localization by Shemer, Tamar Azoulay, Harpaz-Saad, Smadar, Belausov, Eduard, Lovat, Nicole, Krokhin, Oleg, Spicer, Victor, Standing, Kenneth G, Goldschmidt, Eliezer E, Eyal, Yoram

“…Fruit color-break is the visual manifestation of the developmentally regulated transition of chloroplasts to chromoplasts during fruit ripening and often…”
Get full text

Deamidation of -Asn-Gly- Sequences during Sample Preparation for Proteomics:  Consequences for MALDI and HPLC-MALDI Analysis by Krokhin, Oleg V., Antonovici, Mihaela, Ens, Werner, Wilkins, John A., Standing, Kenneth G.

“…We find that peptides containing -Asn-Gly- sequences typically show ∼70−80% degree of deamidation after standard overnight (∼12 h) tryptic digestion at 37 °C…”
Get full text

Sequence-Specific Retention Calculator. A Family of Peptide Retention Time Prediction Algorithms in Reversed-Phase HPLC:  Applicability to Various Chromatographic Conditions and Columns by Spicer, Vic, Yamchuk, Andriy, Cortens, John, Sousa, Sandra, Ens, Werner, Standing, Kenneth G, Wilkins, John A, Krokhin, Oleg V

“…Separation selectivity of C18 reversed-phase columns from different manufacturers has been compared to evaluate the applicability of our sequence-specific…”
Get full text

MALDI Quadrupole Time-of-Flight Mass Spectrometry:  A Powerful Tool for Proteomic Research by Shevchenko, Andrej, Loboda, Alexander, Shevchenko, Anna, Ens, Werner, Standing, Kenneth G

“…A MALDI QqTOF mass spectrometer has been used to identify proteins separated by one-dimensional or two-dimensional gel electrophoresis at the femtomole level…”
Get full text

Use of Peptide Retention Time Prediction for Protein Identification by off-line Reversed-Phase HPLC−MALDI MS/MS by Krokhin, Oleg V, Ying, Stephen, Cortens, John P, Ghosh, Dhiman, Spicer, Victor, Ens, Werner, Standing, Kenneth G, Beavis, Ronald C, Wilkins, John A

“…A new algorithm, sequence-specific retention calculator, was developed to predict retention time of tryptic peptides during RP HPLC fractionation on C18, 300-Å…”
Get full text

Lectin Affinity as an Approach to the Proteomic Analysis of Membrane Glycoproteins by Ghosh, Dhiman, Krokhin, Oleg, Antonovici, Mihaela, Ens, Werner, Standing, Kenneth G, Beavis, Ronald C, Wilkins, John A

“…The aim was to determine the proportion of membrane glycoproteins captured using concanavalin A or wheat germ agglutinin lectin affinity chromatography…”
Get full text

Quantitative Proteomic Analysis Using a MALDI Quadrupole Time-of-Flight Mass Spectrometer by Griffin, Timothy J, Gygi, Steven P, Rist, Beate, Aebersold, Ruedi, Loboda, Alexander, Jilkine, Alexandra, Ens, Werner, Standing, Kenneth G

“…We describe an approach to the quantitative analysis of complex protein mixtures using a MALDI quadrupole time-of-flight (MALDI QqTOF) mass spectrometer and…”
Get full text

Heavy Petroleum Composition. 3. Asphaltene Aggregation by McKenna, Amy M, Donald, Lynda J, Fitzsimmons, Jade E, Juyal, Priyanka, Spicer, Victor, Standing, Kenneth G, Marshall, Alan G, Rodgers, Ryan P

“…Molecular characterization of asphaltenes by conventional analytical techniques is a challenge because of their compositional complexity, high heteroatom…”
Get full text

Tat peptide-calmodulin binding studies and bioinformatics of HIV-1 protein-calmodulin interactions by McQueen, Peter, Donald, Lynda J., Vo, Thach N., Nguyen, Dung H., Griffiths, Heather, Shojania, Shaheen, Standing, Kenneth G., O'Neil, Joe D.

“…The human immunodeficiency virus type 1 (HIV‐1) genome encodes 18 proteins and 2 peptides. Four of these proteins encode high‐affinity calmodulin‐binding sites…”
Get full text

Site-specific N-glycosylation analysis: matrix-assisted laser desorption/ionization quadrupole-quadrupole time-of-flight tandem mass spectral signatures for recognition and identification of glycopeptides by Krokhin, Oleg, Ens, Werner, Standing, Kenneth G., Wilkins, John, Perreault, Hélène

“…The identification of glycosylation sites in proteins is often possible through a combination of proteolytic digestion, separation, mass spectrometry (MS) and…”
Get full text

Application of the StrOligo algorithm for the automated structure assignment of complex N-linked glycans from glycoproteins using tandem mass spectrometry by Ethier, Martin, Saba, Julian A., Spearman, Maureen, Krokhin, Oleg, Butler, Michael, Ens, Werner, Standing, Kenneth G., Perreault, Hélène

“…Oligosaccharides associated with proteins are known to give these molecules specific conformations and functions. Analysis of proteins would not be complete…”
Get full text

The β-Lactamase Gene Regulator AmpR Is a Tetramer That Recognizes and Binds the d-Ala-d-Ala Motif of Its Repressor UDP-N-acetylmuramic Acid (MurNAc)-pentapeptide by Vadlamani, Grishma, Thomas, Misty D., Patel, Trushar R., Donald, Lynda J., Reeve, Thomas M., Stetefeld, Jörg, Standing, Kenneth G., Vocadlo, David J., Mark, Brian L.

“…Inducible expression of chromosomal AmpC β-lactamase is a major cause of β-lactam antibiotic resistance in the Gram-negative bacteria Pseudomonas aeruginosa…”
Get full text

Practical Implementation of 2D HPLC Scheme with Accurate Peptide Retention Prediction in Both Dimensions for High-Throughput Bottom-Up Proteomics by Dwivedi, Ravi C, Spicer, Vic, Harder, Michael, Antonovici, Mihaela, Ens, Werner, Standing, Kenneth G, Wilkins, John A, Krokhin, Oleg V

“…We describe the practical implementation of a new RP (pH 10 − pH 2) 2D HPLC−ESI/MS scheme for large-scale bottom-up analysis in proteomics. When compared to…”
Get full text

Peptide and protein de novo sequencing by mass spectrometry by Standing, Kenneth G

“…Although the advent of large-scale genomic sequencing has greatly simplified the task of determining the primary structures of peptides and proteins, the…”
Get full text

Characterizing degradation products of peptides containing N-terminal Cys residues by (off-line high-performance liquid chromatography)/matrix-assisted laser desorption/ionization quadrupole time-of-flight measurements by Krokhin, Oleg V., Ens, Werner, Standing, Kenneth G.

“…A transformation analogous to the well‐known conversion of an N‐terminal glutamine residue to pyroglutamic acid is the cyclization of an N‐terminal…”
Get full text

Predicting Retention Time Shifts Associated with Variation of the Gradient Slope in Peptide RP-HPLC by Spicer, Vic, Grigoryan, Marine, Gotfrid, Alexander, Standing, Kenneth G, Krokhin, Oleg V

“…We have developed a sequence-specific model for predicting slopes (S) in the fundamental equation of linear solvent strength theory for the reversed-phase HPLC…”
Get full text

Automated structural assignment of derivatized complex N-linked oligosaccharides from tandem mass spectra by Ethier, Martin, Saba, Julian A., Ens, Werner, Standing, Kenneth G., Perreault, Hélène

“…Glycoprotein function is controlled by several biological factors, one of them being the structure of carbohydrate chains (glycans) attached to specific amino…”
Get full text

Charting the Proteomes of Organisms with Unsequenced Genomes by MALDI-Quadrupole Time-of-Flight Mass Spectrometry and BLAST Homology Searching by Shevchenko, Andrej, Sunyaev, Shamil, Loboda, Alexander, Shevchenko, Anna, Bork, Peer, Ens, Werner, Standing, Kenneth G

“…MALDI-quadrupole time-of-flight mass spectrometry was applied to identify proteins from organisms whose genomes are still unknown. The identification was…”
Get full text

A Study of Immunoglobulin G Glycosylation in Monoclonal and Polyclonal Species by Electrospray and Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry by Saba, Julian A., Kunkel, Jeremy P., Jan, David C.H., Ens, Werner E., Standing, Kenneth G., Butler, Michael, Jamieson, James C., Perreault, Hélène

“…N-linked oligosaccharides were released from human and bovine polyclonal immunoglobulin G (IgG) obtained from commercial sources and also from a monoclonal…”
Get full text

Mass spectrometric characterization of proteins from the SARS virus: a preliminary report by Krokhin, Oleg, Li, Yan, Andonov, Anton, Feldmann, Heinz, Flick, Ramon, Jones, Steven, Stroeher, Ute, Bastien, Nathalie, Dasuri, Kumar V N, Cheng, Keding, Simonsen, J Neil, Perreault, Hélène, Wilkins, John, Ens, Werner, Plummer, Frank, Standing, Kenneth G

“…A new coronavirus has been implicated as the causative agent of severe acute respiratory syndrome (SARS). We have used convalescent sera from several SARS…”
Get full text