Search Results - "SPROAT, B"

PEGylation of biodegradable dextran nanogels for siRNA delivery by Naeye, B., Raemdonck, K., Remaut, K., Sproat, B., Demeester, J., De Smedt, S.C.

“…Delivering intact small interfering RNA (siRNA) into the cytoplasm of targeted cells in vivo is considered a major obstacle in the development of clinically…”
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Social Emotional Learning for Teachers (SELF-T): A Short-term, Online Intervention to Increase Early Childhood Educators' Resilience by Lang, Sarah N., Jeon, Lieny, Sproat, Eliza B., Brothers, Brennan E., Buettner, Cynthia K.

“…Early childhood teachers' social emotional well-being is important for their quality of life, and is associated with their classroom practices, their…”
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Structural and Mechanistic Basis of Pre- and Posttransfer Editing by Leucyl-tRNA Synthetase by Lincecum, Tommie L., Tukalo, Michael, Yaremchuk, Anna, Mursinna, Richard S., Williams, Amy M., Sproat, Brian S., Van Den Eynde, Wendy, Link, Andreas, Van Calenbergh, Serge, Grøtli, Morten, Martinis, Susan A., Cusack, Stephen

“…The aminoacyl-tRNA synthetases link tRNAs with their cognate amino acid. In some cases, their fidelity relies on hydrolytic editing that destroys incorrectly…”
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Factual exactitude by Sproat, John B.

“…Perhaps it is a semantic point, but factual exactitude is as important, or even more important, than grammatical correctness (VR, November 30, 2013, vol 173, p…”
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A synthetic, chemically modified ribozyme eliminates amelogenin, the major translation product in developing mouse enamel in vivo by Lyngstadaas, S. P., Risnes, S., Sproat, B. S., Thrane, P. S., Prydz, H. P.

“…Ribozymes are small RNA structures capable of cleaving RNA target molecules in a catalytic fashion. Designed ribozymes can be targeted to specific mRNAs,…”
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Antisense oligonucleotides made of 2'- O-alkylRNA: their properties and applications in RNA biochemistry by Lamond, Angus I., Sproat, Brian S.

“…Oligo(2'- O-alkylribonucleotides) have been developed recently as novel oligonucleotide analogues with properties that enhance their use as antisense probes…”
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Nuclease resistant ribozymes with high catalytic activity by Paolella, G., Sproat, B.S., Lamond, A.I.

“…Hammerhead ribozymes are efficient RNA enzymes characterized by a typical hammerhead secondary structure and a number of conserved bases. Little is known about…”
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2'-O-Alkyl Oligoribonucleotides as Antisense Probes by Iribarren, Adolfo M., Sproat, Brian S., Neuner, Philippe, Sulston, Ingrid, Ryder, Ursula, Lamond, Angus I.

“…2'-O-Methyl oligoribonucleotides have recently been introduced as antisense probes for studying RNA processing and for affinity purification of RNA-protein…”
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Comparative evaluation of seven oligonucleotide analogs as potential antisense agents by Morvan, F, Porumb, H, Degols, G, Lefebvre, I, Pompon, A, Sproat, B. S, Rayner, B, Malvy, C, Lebleu, B, Imbach, J. L

“…12-Mer analogues, representative of seven different classes of structurally modified oligonucleotides and complementary to the same target, have been compared…”
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Fast and simple purification of chemically modified hammerhead ribozymes using a lipophilic capture tag by Sproat, Brian S., Rupp, Thomas, Menhardt, Norbert, Keane, Doreen, Beijer, Barbro

“…A new type of 5′-lipophilic capture tag is described, enabling the facile reverse phase HPLC purification of chemically modified hammerhead ribozymes…”
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In vitro reconstitution of mammalian U2 and U5 snRNPs active in splicing: Sm proteins are functionally interchangeable and are essential for the formation of functional U2 and U5 snRNPs by Ségault, V., Will, C. L., Sproat, B. S., Lührmann, R.

“…An in vitro reconstitution/splicing complementation system has been developed which has allowed the investigation of the role of mammalian U2 and U5 snRNP…”
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How glutaminyl-tRNA synthetase selects glutamine by Rath, Virginia L, Silvian, Laura F, Beijer, Barbro, Sproat, Brian S, Steitz, Thomas A

“…Background: Aminoacyl-tRNA synthetases covalently link a specific amino acid to the correct tRNA. The fidelity of this reaction is essential for accurate…”
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Targeted snRNP depletion reveals an additional role for mammalian U1 snRNP in spliceosome assembly by Barabino, S M, Blencowe, B J, Ryder, U, Sproat, B S, Lamond, A I

“…HeLa cell nuclear splicing extracts have been prepared that are specifically and efficiently depleted of U1, U2, or U4/U6 snRNPs by antisense affinity…”
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Antisense probing of the human U4/U6 snRNP with biotinylated 2'-OMe RNA oligonucleotides by Blencowe, B J, Sproat, B S, Ryder, U, Barabino, S, Lamond, A I

“…We have used antisense 2'-OMe RNA oligonucleotides carrying four 5'-terminal biotin residues to probe the structure and function of the human U4/U6 snRNP. Nine…”
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In vivo detection of snRNP‐rich organelles in the nuclei of mammalian cells by Carmo‐Fonseca, M., Pepperkok, R., Sproat, B.S., Ansorge, W., Swanson, M.S., Lamond, A.I.

“…The in vivo distribution of snRNPs has been analysed by microinjecting fluorochrome‐labelled antisense probes into the nuclei of live HeLa and 3T3 cells…”
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A protected biotin containing deoxycytidine building block for solid phase synthesis of biotinylated oligonucleotides by PIELES, U, SPROAT, B. S, LAMM, G. M

“…The synthesis of a modified 2'-deoxycytidine-3'-O-phosphoramidite carrying an N-t-butylbenzoyl protected biotin on a long polar spacer arm attached to the 4-N…”
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Withdrawal of routine brucellosis testing in beef herds by Sproat, John B.

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Antisense probes containing 2-aminoadenosine allow efficient depletion of U5 snRNP from HeLa splicing extracts by LAMM, G. M, BLENCOWE, B. J, SPROAT, B. S, IRIBARREN, A. M, RYDER, U, LAMOND, A. I

“…RNA duplexes containing the modified base 2-amino-adenine in place of adenine are stabilized through the formation of three hydrogen bonds in 2-amino A.U base…”
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Synthesis of 2'-O-alkyloligoribonucleotides by Sproat, B S

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Preparation of a novel psoralen containing deoxyadenosine building block for the facile solid phase synthesis of psoralen-modified oligonucleotides for a sequence specific crosslink to a given target sequence by PIELES, U, SPROAT, B. S, NEUNER, P, CRAMER, F

“…4,5',8-Trimethylpsoralen was attached to the C8-position of deoxyadenosine via a sulfur atom and a five carbon atom linker. The modified deoxyadenosine was…”
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