Search Results - "SOBEK, H"

Understanding the viral load during the synthesis and after rebinding of virus imprinted particles via real-time quantitative PCR by Gast, M, Kühner, S, Sobek, H, Mizaikoff, B

“…In the present study, virus imprinted particles have been synthesized for recognizing and specifically binding viruses. These materials may be used for…”
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Domain exchange: chimeras of Thermus aquaticus DNA polymerase, Escherichia coli DNA polymerase I and Thermotoga neapolitana DNA polymerase by Villbrandt, B., Sobek, H., Frey, B., Schomburg, D.

“…The intervening domain of the thermostable Thermus aquaticus DNA polymerase (Taq polymerase), which has no catalytic activity, has been exchanged for the 3′–5′…”
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Heat-labile uracil-DNA glycosylase: purification and characterization by Sobek, H., Schmidt, M., Frey, B., Kaluza, K.

“…A uracil-DNA glycosylase (UNG) from a psychrophilic marine bacterium (BMTU 3346) has been purified to apparent homogeneity. The enzyme has a molecular weight…”
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Molecular cloning, sequencing, and expression of the heat-labile uracil-DNA glycosylase from a marine psychrophilic bacterium, strain BMTU3346 by JAEGER, S, SCHMUCK, R, SOBEK, H

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Molecular cloning, sequency, and expression of the heat-labile uracil-DNA glycosylase from a marine psychrophilic bacterium, strain BMTU3346 by Jaeger, S, Schmuck, R, Sobek, H

“…The gene encoding a heat-labile uracil-DNA glycosylase (UDG) from a psychrophilic, gram-positive marine strain (BMTU3346) has been cloned, sequenced, and…”
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Purification and characterization of a heat-stable esterase from the thermoacidophilic archaebacterium Sulfolobus acidocaldarius by Sobek, H, Görisch, H

“…A heat-stable esterase has been purified 1080-fold to electrophoretic homogeneity from Sulfolobus acidocaldarius, a thermoacidophilic archaebacterium; 20% of…”
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The metal-ion-free oxidoreductase from Streptomyces aureofaciens has an α/β hydrolase fold by Hecht, H.J, Sobek, H, Haag, T, Pfeifer, O, van Pée, K.-H

“…The crystal structure of the bromoperoxidase A2 from Streptomyces aureofaciens (ATCC 10762) has been determined by isomorphous replacement and refined to 2.05…”
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Further kinetic and molecular characterization of an extremely heat-stable carboxylesterase from the thermoacidophilic archaebacterium Sulfolobus acidocaldarius by Sobek, H, Görisch, H

“…The carboxylesterase (serine esterase, EC 3.1.1.1) from Sulfolobus acidocaldarius was purified 940-fold to homogeneity by an improved purification procedure…”
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Rational protein engineering and industrial application: structure prediction by homology and rational design of protein-variants with improved 'washing performance'--the alkaline protease from Bacillus alcalophilus by Aehle, W, Sobek, H, Amory, A, Vetter, R, Wilke, D, Schomburg, D

“…The successful attempt is presented to engineer an enzyme with respect to its technical application by the use of computer-aided protein design techniques…”
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Phosphoenolpyruvate-dependent phosphotransferase system of Staphylococcus aureus: factor IIIlac, a trimeric phospho-carrier protein that also acts as a phase transfer catalyst by Deutscher, J, Beyreuther, K, Sobek, H, Stueber, K, Hengstenberg, W

“…Factor IIIlac (FIII) consists of three identical subunits. It could be shown that each of the subunits carries a phosphoryl group upon phosphorylation (P-FIII)…”
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X-ray structure determination and comparison of two crystal forms of a variant (Asn115Arg) of the alkaline protease from Bacillus alcalophilus refined at 1.85 A resolution by Sobek, H, Hecht, H J, Aehle, W, Schomburg, D

“…The X-ray structure determination, refinement and comparison of two crystal forms of a variant (Asn115Arg) of the alkaline protease from Bacillus alcalophilus…”
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Crystal structure of an alkaline protease from Bacillus alcalophilus at 2.4 Å resolution by SOBEK, H, HECHT, H. J, HOFMANN, B, AEHLE, W, SCHOMBURG, D

“…The crystal structure of an alkaline protease from Bacillus alcalophilus has been determined by X-ray diffraction at 2.4 A resolution. The enzyme crystallizes…”
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Crystallization and preliminary X-ray data of bromoperoxidase from Streptomyces aureofaciens ATCC 10762 by Sobek, H, Haag, T, Pfeifer, O, Schomburg, D, Lingens, F, van Pée, K H

“…Bromoperoxidase from Streptomyces aureofaciens ATCC 10762, a non-haem haloperoxidase, has been crystallized using the hanging drop method. Preliminary X-ray…”
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Staphylococcal phosphoenolpyruvate-dependent phosphotransferase system: purification and characterization of a defective lactose-specific factor III protein by Sobek, H. M, Stueber, K, Beyreuther, K, Hengstenberg, W, Deutscher, Josef

“…Factor III protein specific for lactose (FIII super(Lac)) is part of the lactose-specific phosphotransferase system of Staphylococcus aureus . It is…”
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Amino acid sequence of the amphiphilic phosphocarrier protein factor IIILac of the lactose-specific phosphotransferase system of Staphylococcus aureus by Stueber, K, Deutscher, Joseph, Sobek, H. M, Hengstenberg, W, Beyreuther, K

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Domain exchange: chimeras of DNA polymerase, Escherichia coli DNA polymerase I and Thermotoga neapolitana DNA polymerase by Villbrandt, B, Sobek, H, Frey, B, Schomburg, D

“…The intervening domain of the thermostable Thermus aquaticus DNA polymerase (Taq polymerase), which has no catalytic activity, has been exchanged for the 3'-5'…”
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PCR performance of the B-type DNA polymerase from the thermophilic euryarchaeon Thermococcus aggregans improved by mutations in the Y-GG/A motif by Böhlke, K, Pisani, F M, Vorgias, C E, Frey, B, Sobek, H, Rossi, M, Antranikian, G

“…The effect of mutations in the highly conserved Y-GG/A motif of B-type DNA polymerases was studied in the DNA polymerase from the hyperthermophilic…”
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A new peptide-affinity tag for the detection and affinity purification of recombinant proteins with a monoclonal antibody by Böldicke, Thomas, Struck, Friedhelm, Schaper, Fred, Tegge, Werner, Sobek, Harald, Villbrandt, Britta, Lankenau, Petra, Böcher, Michael

“…A monoclonal anti-peptide antibody (2E11) was raised against the synthetic peptide 38 (C-L-D-K-S-G-L-P-S-D-R-F-F-A) representing a part of the variable region…”
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A positive selection vector for cloning of long polymerase chain reaction fragments based on a lethal mutant of the crp gene of Escherichia coli by Schlieper, D, von Wilcken-Bergmann, B, Schmidt, M, Sobek, H, Müller-Hill, B

“…We have constructed a cloning vector with a tight positive selection for recombinant clones in Escherichia coli. The positive selection pressure results from a…”
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Evaluation of protein 3-D structure prediction: Comparison of modelled and X-ray structure of an alkaline serine protease by Aehle, Wolfgang, Sobek, Harald, Schomburg, Dietmar

“…We describe the modelling of the structure of the highly alkaline subtilisin protease OPTICLEAN from Bacillus alcalophilus. The model was developed through…”
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