Search Results - "SMITH, Steven B"

Recent advances in optical tweezers by Moffitt, Jeffrey R, Chemla, Yann R, Smith, Steven B, Bustamante, Carlos

“…It has been over 20 years since the pioneering work of Arthur Ashkin, and in the intervening years, the field of optical tweezers has grown tremendously…”
Get full text

The Cervicovaginal Microbiota-Host Interaction Modulates Chlamydia trachomatis Infection by Edwards, Vonetta L, Smith, Steven B, McComb, Elias J, Tamarelle, Jeanne, Ma, Bing, Humphrys, Michael S, Gajer, Pawel, Gwilliam, Kathleen, Schaefer, Alison M, Lai, Samuel K, Terplan, Mishka, Mark, Katrina S, Brotman, Rebecca M, Forney, Larry J, Bavoil, Patrik M, Ravel, Jacques

“…The mechanism(s) by which -dominated cervicovaginal microbiota provide a barrier to infection remain(s) unknown. Here we evaluate the impact of different spp…”
Get full text

A Temperature-Jump Optical Trap for Single-Molecule Manipulation by de Lorenzo, Sara, Ribezzi-Crivellari, Marco, Arias-Gonzalez, J. Ricardo, Smith, Steven B., Ritort, Felix

“…To our knowledge, we have developed a novel temperature-jump optical tweezers setup that changes the temperature locally and rapidly. It uses a heating laser…”
Get full text

Single-molecule derivation of salt dependent base-pair free energies in DNA by Huguet, Josep M., Bizarro, Cristiano V., Forns, Núria, Smith, Steven B., Bustamante, Carlos, Ritort, Felix, Uhlenbeck, Olke C.

“…Accurate knowledge of the thermodynamic properties of nucleic acids is crucial to predicting their structure and stability. To date most measurements of…”
Get full text

Identification of common biological pathways and drug targets across multiple respiratory viruses based on human host gene expression analysis by Smith, Steven B, Dampier, William, Tozeren, Aydin, Brown, James R, Magid-Slav, Michal

“…Pandemic and seasonal respiratory viruses are a major global health concern. Given the genetic diversity of respiratory viruses and the emergence of drug…”
Get full text

The vaginal microbiota, host defence and reproductive physiology by Smith, Steven B, Ravel, Jacques

“…The interaction between the human host and the vaginal microbiota is highly dynamic. Major changes in the vaginal physiology and microbiota over a woman's…”
Get full text

Tension induces a base-paired overstretched DNA conformation by Bosaeus, Niklas, El-Sagheer, Afaf H, Brown, Tom, Smith, Steven B, Åkerman, Björn, Bustamante, Carlos, Nordén, Bengt

“…Mixed-sequence DNA molecules undergo mechanical overstretching by approximately 70% at 60–70 pN. Since its initial discovery 15 y ago, a debate has arisen as…”
Get full text

Equilibrium Information from Nonequilibrium Measurements in an Experimental Test of Jarzynski's Equality by Liphardt, Jan, Dumont, Sophie, Smith, Steven B., Tinoco, Ignacio, Bustamante, Carlos

“…Recent advances in statistical mechanical theory can be used to solve a fundamental problem in experimental thermodynamics. In 1997, Jarzynski proved an…”
Get full text

DNA Translocation and Loop Formation Mechanism of Chromatin Remodeling by SWI/SNF and RSC by Zhang, Yongli, Smith, Corey L., Saha, Anjanabha, Grill, Stephan W., Mihardja, Shirley, Smith, Steven B., Cairns, Bradley R., Peterson, Craig L., Bustamante, Carlos

“…ATP-dependent chromatin-remodeling complexes (remodelers) modulate gene transcription by regulating the accessibility of highly packaged genomic DNA. However,…”
Get full text

The bacteriophage Φ29 portal motor can package DNA against a large internal force by SMITH, Douglas E, TANS, Sander J, SMITH, Steven B, GRIMES, Shelley, ANDERSON, Dwight L, BUSTAMANTE, Carlos

Get full text

Structural transitions and elasticity from torque measurements on DNA by Bustamante, Carlos, Bryant, Zev, Stone, Michael D, Gore, Jeff, Smith, Steven B, Cozzarelli, Nicholas R

“…Knowledge of the elastic properties of DNA is required to understand the structural dynamics of cellular processes such as replication and transcription…”
Get full text

Reversible Unfolding of Single RNA Molecules by Mechanical Force by Liphardt, Jan, Onoa, Bibiana, Smith, Steven B., Tinoco, Ignacio, Bustamante, Carlos

“…Here we use mechanical force to induce the unfolding and refolding of single RNA molecules: a simple RNA hairpin, a molecule containing a three-helix junction,…”
Get full text

Force Unfolding Kinetics of RNA Using Optical Tweezers. I. Effects of Experimental Variables on Measured Results by Wen, Jin-Der, Manosas, Maria, Li, Pan T.X., Smith, Steven B., Bustamante, Carlos, Ritort, Felix, Tinoco, Ignacio

“…Experimental variables of optical tweezers instrumentation that affect RNA folding/unfolding kinetics were investigated. A model RNA hairpin, P5ab, was…”
Get full text

Host response to respiratory bacterial pathogens as identified by integrated analysis of human gene expression data by Smith, Steven B, Magid-Slav, Michal, Brown, James R

“…Respiratory bacterial pathogens are one of the leading causes of infectious death in the world and a major health concern complicated by the rise of…”
Get full text

Overstretching B-DNA: The Elastic Response of Individual Double-Stranded and Single-Stranded DNA Molecules by Smith, Steven B., Cui, Yujia, Bustamante, Carlos

“…Single molecules of double-stranded DNA (dsDNA) were stretched with force-measuring laser tweezers. Under a longitudinal stress of ∼65 piconewtons (pN), dsDNA…”
Get full text

Single-molecule studies of the effect of template tension on T7 DNA polymerase activity by Bustamante, Carlos, Wuite, Gijs J.L, Smith, Steven B, Young, Mark, Keller, David

“…T7 DNA polymerase catalyses DNA replication in vitro at rates of more than 100 bases per second and has a 3′→5′ exonuclease (nucleotide removing) activity at a…”
Get full text

Probing the Mechanical Folding Kinetics of TAR RNA by Hopping, Force-Jump, and Force-Ramp Methods by Li, Pan T.X., Collin, Delphine, Smith, Steven B., Bustamante, Carlos, Tinoco, Ignacio

“…Mechanical unfolding and refolding of single RNA molecules have previously been observed in optical traps as sudden changes in molecular extension. Two methods…”
Get full text

Ionic Effects on the Elasticity of Single DNA Molecules by Baumann, Christoph G., Smith, Steven B., Bloomfield, Victor A., Bustamante, Carlos

“…We used a force-measuring laser tweezers apparatus to determine the elastic properties of λ -bacteriophage DNA as a function of ionic strength and in the…”
Get full text

Chirality Sensing by Escherichia coli Topoisomerase IV and the Mechanism of Type II Topoisomerases by Stone, Michael D., Bryant, Zev, Crisona, Nancy J., Smith, Steven B., Vologodskii, Alexander, Bustamante, Carlos, Cozzarelli, Nicholas R.

“…Escherichia coli topoisomerase (Topo) IV is an essential type II Topo that removes DNA entanglements created during DNA replication. Topo IV relaxes (+)…”
Get full text

Raymond Aron: Philosopher of Liberties by Smith, Steven B.

“…The name of Raymond Aron is often strangely missing from the canon of the twentieth-century’s great philosophers. He is sometimes thought of as a “Cold War…”
Get full text