In vitro transdifferentiation of human fetal type II cells toward a type I-like cell

In vitro transdifferentiation of human fetal type II cells toward a type I-like cell

For alveolar type I cells, phenotype plasticity and physiology other than gas exchange await further clarification due to in vitro study difficulties in isolating and maintaining type I cells in primary culture. Using an established in vitro model of human fetal type II cells, in which the type II p...

Full description

Saved in:
Bibliographic Details
Published in:Pediatric research Vol. 61; no. 4; pp. 404 - 409
Main Authors: FOSTER, Cherie D, VARGHESE, Linda S, SKALINA, Rachel B, GONZALES, Linda W, GUTTENTAG, Susan H
Format: Journal Article
Language:English
Published: Hagerstown, MD Lippincott Williams & Wilkins 01-04-2007
Subjects:
Biological and medical sciences
Cell Differentiation - physiology
Cell Membrane Permeability
Cell Proliferation
Cell Survival
Cells, Cultured
Fetus - cytology
General aspects
Humans
Medical sciences
Pulmonary Alveoli - cytology
Respiratory Mucosa - cytology
Human
Pediatrics
Type
Fetal cell
Transdifférentiation
A-Cell
In vitro
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:For alveolar type I cells, phenotype plasticity and physiology other than gas exchange await further clarification due to in vitro study difficulties in isolating and maintaining type I cells in primary culture. Using an established in vitro model of human fetal type II cells, in which the type II phenotype is induced and maintained by adding hormones, we assessed for transdifferentiation in culture toward a type I-like cell with hormone removal for up to 144 h, followed by electron microscopy, permeability studies, and RNA and protein analysis. Hormone withdrawal resulted in diminished type II cell characteristics, including decreased microvilli, lamellar bodies, and type II cell marker RNA and protein. There was a simultaneous increase in type I characteristics, including increased epithelial cell barrier function indicative of a tight monolayer and increased type I cell marker RNA and protein. Our results indicate that hormone removal from cultured human fetal type II cells results in transdifferentiation toward a type I-like cell. This model will be useful for continued in vitro studies of human fetal alveolar epithelial cell differentiation and phenotype plasticity.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0031-3998
1530-0447
DOI:10.1203/pdr.0b013e3180332c6d