IN VITRO CULTURE STUDIES ON STEVIA REBAUDIANA

IN VITRO CULTURE STUDIES ON STEVIA REBAUDIANA

Shoot apex, nodal, and leaf explants of Stevia rebaudiana Bertoni can regenerate shoots when cultured on Murashige and Skoog (MS) medium supplemented with 6-benzyladenine (BA; 8.87 μM) and indole-3-acetic acid (5.71 μM). Rooting of the in vitro-derived shoots could be achieved following subculture o...

Full description

Saved in:
Bibliographic Details
Published in:In vitro cellular & developmental biology. Plant Vol. 39; no. 5; pp. 520 - 523
Main Authors: SIVARAM, LATHA, MUKUNDAN, USHA
Format: Journal Article
Language:English
Published: Wallingford Tissue Culture Association 01-09-2003
CABI Publishing
Cambridge University Press
Springer Nature B.V
Subjects:
Auxins
benzyladenine
Biological and medical sciences
Callus
Cultured cells
Diterpenes
explants
Fundamental and applied biological sciences. Psychology
Glycosides
indole acetic acid
indole butyric acid
leaves
micropropagation
Plant cells
Plant physiology and development
Plantlets
Plants
Research Reports
Rooting
shoots
Stevia rebaudiana
stevioside
survival rate
tissue culture
Tissue cultures, protoplasts
Vermiculite
Dicotyledones
tissue culture
Angiospermae
Spermatophyta
Micropropagation
Stevia rebaudiana
stevioside
Compositae
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Shoot apex, nodal, and leaf explants of Stevia rebaudiana Bertoni can regenerate shoots when cultured on Murashige and Skoog (MS) medium supplemented with 6-benzyladenine (BA; 8.87 μM) and indole-3-acetic acid (5.71 μM). Rooting of the in vitro-derived shoots could be achieved following subculture onto auxin-containing medium. A survival rate of 70% was recorded at the hardening phase on the substrate cocopeat. The presence of the sweet diterpene glycosides, viz. stevioside and rebaudioside, was confirmed in the in vitro-derived tissues of Stevia using HPTLC techniques. Callus cultured on agar-solidified MS medium supplemented with BA (8.87 μM) and indole-3-butyric acid (9.80 μM) showed the highest sweetener content.
Bibliography:http://dx.doi.org/10.1079%2FIVP2003438
ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:1054-5476
1475-2689
DOI:10.1079/IVP2003438