Evaluation of biodegradation and biocompatibility of collagen/chitosan/alkaline phosphatase biopolymeric membranes

Evaluation of biodegradation and biocompatibility of collagen/chitosan/alkaline phosphatase biopolymeric membranes

The aim of this study was to develop a new variant of membranes based on collagen (COL), chitosan (CHI) and alkaline phosphatase (ALP) immobilized and cross-linking with glutaraldehyde (GA) at different concentrations. The biodegradation in the presence of collagenase was investigated. Biocompatibil...

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Bibliographic Details
Published in:Bulletin of materials science Vol. 39; no. 2; pp. 377 - 383
Main Authors: BERTEANU, E, IONITA, D, SIMOIU, M, PARASCHIV, M, TATIA, R, APATEAN, A, SIDOROFF, M, TCACENCO, L
Format: Journal Article
Language:English
Published: Bangalore, India Indian Academy of Sciences 01-04-2016
Springer Nature B.V
Subjects:
Adsorption
Alkaline phosphatase
Biocompatibility
Biodegradation
Biomedical materials
Calcium ions
Chemistry and Materials Science
Chitosan
Collagen
Crosslinking
Cytotoxicity
Dehydrogenases
Engineering
Fibroblasts
Hemoglobin
Low concentrations
Materials Science
Membranes
Molecular biology
Nanostructured materials
Phosphatase
R&D
Reagents
Research & development
Tissue engineering
Toxicity
chitosan
adsorption
biocompatibility
Collagen
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Summary:The aim of this study was to develop a new variant of membranes based on collagen (COL), chitosan (CHI) and alkaline phosphatase (ALP) immobilized and cross-linking with glutaraldehyde (GA) at different concentrations. The biodegradation in the presence of collagenase was investigated. Biocompatibility was evaluated by MTT assay using a mouse fibroblast cell culture type NCTC (clone 929). Non-cross-linked samples were biocompatible and membranes cross-linked with low concentrations of GA (0.04, 0.08%) were also biocompatible. However, high concentrations of GA lead to a decreased biocompatibility. The adsorption behaviour of Ca 2+ ions to all membranes were evaluated using the Freundlich isotherms. Haemolytic studies were performed in order to consider their applications in biomineralization process. By the addition of collagen and ALP to chitosan, the haemolytic index decreases, the COL–CHI–ALP membrane being in the non-haemolytic domain, while the COL–CHI–ALP–GA membrane has a haemolytic index greater than 2, and is slightly haemolytic.
ISSN:0250-4707
0973-7669
DOI:10.1007/s12034-016-1161-7