The pattern of immune cell infiltration in chromoblastomycosis : Involvement of macrophage inflammatory protein-1 alpha/CCL3 and fungi persistence

Chromoblastomycosis (CR) is a subcutaneous chronic mycosis characterized by a granulomatous inflammatory response. However, little is known regarding the pattern of leukocyte subsets in CR and the pathways involved in their recruitment. The objective of this study was to assess the cellular subsets,...

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Published in:	Revista do Instituto de Medicina Tropical de São Paulo Vol. 49; no. 1; pp. 49 - 53
Main Authors:	SA, Vanuza Cristina, SILVA, Tarcilia Aparccida, REIS, Carmelia Matos Santiago, CUNHA, Fernando Queiroz, FIGUEIREDO, Florencio, BOCCA, Anamélia Lorenzetti
Format:	Journal Article
Language:	English
Published:	São Paulo Instituto de Medicina Tropical de São Paulo 01-01-2007 Instituto de Medicina Tropical de Sao Paulo Instituto de Medicina Tropical
Subjects:	Aged Biological and medical sciences Biomarkers Case-Control Studies Chemokine CCL3 Chemokine CCL4 Chromoblastomycoses Chromoblastomycosis - enzymology Chromoblastomycosis - immunology Human mycoses Humans Immunity, Cellular Infectious diseases Langerhans Cells - immunology Lymphocytes - immunology Macrophage Inflammatory Proteins - immunology Macrophages - immunology Medical sciences Middle Aged Mycoses Neutrophils - immunology Nitric Oxide Synthase - immunology Receptors, Chemokine - immunology Superoxide Dismutase - immunology TROPICAL MEDICINE Tropical mycoses Skin disease Chroinoblastomycosis Mycosis Chromoblastomycosis Inflammation Protein Infection Chemokine Fungi Persistence Granuloimas Infiltration Cell Thallophyta Macrophage Granulomas
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Summary:	Chromoblastomycosis (CR) is a subcutaneous chronic mycosis characterized by a granulomatous inflammatory response. However, little is known regarding the pattern of leukocyte subsets in CR and the pathways involved in their recruitment. The objective of this study was to assess the cellular subsets, chemokine, chemokine receptors and enzymes in CR. The inflammatory infiltrate was characterized by immunohistochemistry using antibodies against macrophages (CD68), Langerhans'cells (S100), lymphocytes (CD3, CD4, CD8, CD45RO, CD20 and CD56) and neutrophils (CD15). The expression of MIP-1alpha (Macrophage inflammatory protein-1alpha), chemokine receptors (CXCR3 and CCR1) and enzymes (superoxide dismutase-SOD and nitric oxide synthase-iNOS) was also evaluated by the same method. We observed an increase in all populations evaluated when compared with the controls. Numbers of CD15(+) and CD56(+) were significantly lower than CD3(+), CD4(+), CD20(+) and CD68(+) cells. Statistical analysis revealed an association of fungi numbers with CD3, CD45RO and iNOS-positive cells. Furthermore, MIP-1alpha expression was associated with CD45RO, CD68, iNOS and CXCR3. Our results suggest a possible role of MIP-1alpha and fungi persistence in the cell infiltration in CR sites.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0036-4665 1678-9946 1678-9946 0036-4665
DOI:	10.1590/S0036-46652007000100009