Search Results - "SIGAL, I. S"

Active Human Immunodeficiency Virus Protease is Required for Viral Infectivity by Kohl, Nancy E., Emini, Emilio A., Schleif, William A., Davis, Lenora J., Heimbach, Jill C., Richard A. F. Dixon, Scolnick, Edward M., Sigal, Irving S.

“…Retroviral proteins are synthesized as polyprotein precursors that undergo proteolytic cleavages to yield the mature viral proteins. The role of the human…”
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Identification of Two Serine Residues Involved in Agonist Activation of the β-Adrenergic Receptor by Strader, C D, Candelore, M R, Hill, W S, Sigal, I S, Dixon, R A

“…Pharmacophore mapping of the ligand binding domain of the β-adrenergic receptor has revealed specific molecular interactions which are important for agonist…”
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Structural basis of beta-adrenergic receptor function by Strader, C D, Sigal, I S, Dixon, R A

“…Receptors that mediate their actions by stimulating guanine nucleotide binding regulatory proteins (G proteins) share structural as well as functional…”
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Conserved aspartic acid residues 79 and 113 of the beta-adrenergic receptor have different roles in receptor function by Strader, C D, Sigal, I S, Candelore, M R, Rands, E, Hill, W S, Dixon, R A

“…Deletion mutagenesis experiments have demonstrated that the binding site of the beta-adrenergic receptor involves the hydrophobic core of the protein (Dixon,…”
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Identification of Residues Required for Ligand Binding to the β -adrenergic Receptor by Strader, Catherine D., Sigal, Irving S., Register, R. Bruce, Candelore, Mari Rios, Rands, Elaine, Richard A. F. Dixon

“…The functional significance of conserved polar amino acids within the putative transmembrane region of the β -adrenergic receptor (β AR) was examined by…”
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Structural features required for ligand binding to the beta‐adrenergic receptor by Dixon, R. A., Sigal, I. S., Candelore, M. R., Register, R. B., Scattergood, W., Rands, E., Strader, C. D.

“…On the basis of the homology between the amino acid sequences of the beta‐adrenergic receptor (beta AR) and the opsin proteins we have proposed that the ligand…”
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Mutations that uncouple the beta-adrenergic receptor from Gs and increase agonist affinity by Strader, CD, Dixon, RA, Cheung, AH, Candelore, MR, Blake, AD, Sigal, IS

“…The deletion of residues 239-272 from the hamster beta-adrenergic receptor resulted in a loss of the ability of the receptor, expressed in mouse L cells, to…”
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Identification of Effector Residues and a Neutralizing Epitope of Ha-ras-Encoded p21 by Sigal, Irving S., Gibbs, Jackson B., D'Alonzo, Jill S., Scolnick, Edward M.

“…To identify the amino acid residues of the Harvey (Ha) ras-encoded protein that are involved in protein-protein interactions, we have created a series of…”
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Intrinsic GTPase Activity Distinguishes Normal and Oncogenic ras p21 Molecules by Gibbs, Jackson B., Sigal, Irving S., Poe, Martin, Scolnick, Edward M.

“…The 21-kilodalton protein (p21) encoded by normal cellular Harvey-ras has been expressed in Escherichia coli as a fusion protein by using the pUC8 vector and…”
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A Single Amino Acid Substitution in the β-Adrenergic Receptor Promotes Partial Agonist Activity from Antagonists by Strader, C D, Candelore, M R, Hill, W S, Dixon, R A F, Sigal, I S

“…The family of G-protein-linked receptors includes many important pharmacological targets, of which the β-adrenergic receptor is one of the best characterized…”
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Purification of ras GTPase activating protein from bovine brain by Gibbs, J.B, Schaber, M.D, Allard, W.J, Sigal, I.S, Scolnick, E.M

“…In cytosolic extracts of bovine brain, we detected ras GTPase activating protein (GAP) activity that stimulated the GTP hydrolytic activity of normal c-Ha-ras…”
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Human immunodeficiency virus protease. Bacterial expression and characterization of the purified aspartic protease by Darke, P L, Leu, C T, Davis, L J, Heimbach, J C, Diehl, R E, Hill, W S, Dixon, R A, Sigal, I S

“…The protease of human immunodeficiency virus has been expressed in Escherichia coli and purified to apparent homogeneity. Immunoreactivity toward anti-protease…”
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Cloning of bovine GAP and its interaction with oncogenic ras p21 by Vogel, Ursula S, Dixon, Richard A. F, Schaber, Michael D, Diehl, Ronald E, Marshall, Mark S, Scolnick, Edward M, Sigal, Irving S, Gibbs, Jackson B

“…The plasma membrane-bound mammalian ras proteins of relative molecular mass 21,000 (ras p21) share biochemical and structural properties with other guanine…”
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Mutant ras-Encoded Proteins with Altered Nucleotide Binding Exert Dominant Biological Effects by Sigal, Irving S., Gibbs, Jackson B., D'Alonzo, Jill S., Temeles, Gretchen L., Wolanski, Bohdan S., Socher, Susan H., Scolnick, Edward M.

“…We report that residues Lys-16 and Asp-119 play critical roles in the guanine nucleotide binding and, consequently, the biological function of the…”
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Xenopus Oocyte Germinal-Vesicle Breakdown Induced by [Val12]Ras is Inhibited by a Cytosol-Localized Ras Mutant by Gibbs, Jackson B., Schaber, Michael D., Schofield, Timothy L., Scolnick, Edward M., Sigal, Irving S.

“…The GTPase-activating protein (GAP) has been postulated to function either as a negative regulator or as a possible target protein of Ras in mammalian cells…”
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cDNA for the Human β 2-Adrenergic Receptor: A Protein with Multiple Membrane-Spanning Domains and Encoded by a Gene Whose Chromosomal Location is Shared with that of the Receptor for Platelet-Derived Growth Factor by Kobilka, Brian K., Richard A. F. Dixon, Frielle, Thomas, Dohlman, Henrik G., Bolanowski, Mark A., Sigal, Irving S., Yang-Feng, Teresa L., Francke, Uta, Caron, Marc G., Lefkowitz, Robert J.

“…We have isolated and sequenced a cDNA encoding the human β 2-adrenergic receptor. The deduced amino acid sequence (413 residues) is that of a protein…”
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A C‐terminal domain of GAP is sufficient to stimulate ras p21 GTPase activity by Marshall, M. S., Hill, W. S., Ng, A. S., Vogel, U. S., Schaber, M. D., Scolnick, E. M., Dixon, R. A., Sigal, I. S., Gibbs, J. B.

“…The cDNA for bovine ras p21 GTPase activating protein (GAP) has been cloned and the 1044 amino acid polypeptide encoded by the clone has been shown to bind the…”
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Cloning of the gene and cDNA for mammalian β -adrenergic receptor and homology with rhodopsin by Dixon, Richard A. F, Kobilka, Brian K, Strader, David J, Benovic, Jeffrey L, Dohlman, Henrik G, Frielle, Thomas, Bolanowski, Mark A, Bennett, Carl D, Rands, Elaine, Diehl, Ronald E, Mumford, Richard A, Slater, Eve E, Sigal, Irving S, Caron, Marc G, Lefkowitz, Robert J, Strader, Catherine D

“…The adenylate cyclase system, which consists of a catalytic moiety and regulatory guanine nucleotide-binding proteins, provides the effector mechanism for the…”
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L-687,908, a potent hydroxyethylene containing HIV protease inhibitor by Vacca, Joseph P, Guare, J. P, DeSolms, S. J, Sanders, W. M, Giuliani, E. A, Young, S. D, Darke, P. L, Sigal, I. S, Schleif, W. A

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HIV-1 protease specificity of peptide cleavage is sufficient for processing of gag and pol polyproteins by Darke, P L, Nutt, R F, Brady, S F, Garsky, V M, Ciccarone, T M, Leu, C T, Lumma, P K, Freidinger, R M, Veber, D F, Sigal, I S

“…The mature proteins of retroviruses originate as a result of proteolytic cleavages of polyprotein precursors. Retroviruses encode proteases responsible for…”
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