Over-expression of recombinant human interferon-gamma in high cell density fermentation of Escherichia coli

Human interferon-gamma (hIFN-gamma) was expressed in Escherichia coli BL21(DE3) under the control of the T7 promoter. Glucose was used as the sole source of carbon and energy with simple exponential feeding rate in fed-batch process. Cell density of recombinant E. coli was reached to 100 g dry wt l(...

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Bibliographic Details
Published in:	Biotechnology letters Vol. 25; no. 23; pp. 1989 - 1992
Main Authors:	KHALIZADEH, R, SHOIAOSADATI, S. A, BAHRAMI, A, MAGHSOUDI, N
Format:	Journal Article
Language:	English
Published:	Dordrecht Springer 01-12-2003 Springer Nature B.V
Subjects:	Bacteria Biological and medical sciences Bioreactors - microbiology Biotechnology Cell Count Cell Culture Techniques - methods Cell Division E coli Escherichia coli Escherichia coli - enzymology Escherichia coli - genetics Escherichia coli - growth & development Feeding rates Fermentation Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Bacterial - physiology Glucose - metabolism Humans Interferon-gamma - biosynthesis Interferon-gamma - genetics Microbiology Models, Biological Protein Engineering - methods Recombinant Proteins - biosynthesis Specific yield Human plasmid stability Stability Escherichia coli recombinant human interferon-gamma Gene overexpression Fermentation Plasmid Bacteria Recombinant protein Semicontinuous fed-batch fermentation Enterobacteriaceae Gamma interferon
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Summary:	Human interferon-gamma (hIFN-gamma) was expressed in Escherichia coli BL21(DE3) under the control of the T7 promoter. Glucose was used as the sole source of carbon and energy with simple exponential feeding rate in fed-batch process. Cell density of recombinant E. coli was reached to 100 g dry wt l(-1) under both constant (0.12 h(-1)) and variable (0.12-0.52 h(-1)) specific growth rates. In the variable specific growth rate fed-batch process, plasmid stability and specific yield of rhIFN-gamma were greater than constant specific growth rate fed-batch process. The final specific yield and overall productivity of rhIFN-gamma were 0.35 +/- 0.02 g rhIFN-gamma g(-1) dry cell wt and 0.9 +/- 0.05 g rhIFN-gamma l(-1) h(-1) in the variable specific growth rate fed-batch process, respectively.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0141-5492 1573-6776
DOI:	10.1023/B:BILE.0000004390.98648.25