The dependence on chloride ions of the loop diuretic sensitive component of passive sodium efflux from human red cells

The dependence on chloride ions of the loop diuretic sensitive component of passive sodium efflux from human red cells

A study has been made of the passive (ouabain-insensitive) Na efflux from human red cells. The inhibition by loop diuretics (furosemide, piretanide and bumetanide) was additive with respect to ouabain and non-additive with respect to each other. They inhibited with high affinity in the sequence bume...

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Bibliographic Details
Published in:Pflügers Archiv Vol. 406; no. 3; pp. 333 - 339
Main Authors: SHENNAM, D. B, CHIPPERFIELD, A. R
Format: Journal Article
Language:English
Published: Heidelberg Springer 01-03-1986
Subjects:
Anions - physiology
Biological and medical sciences
Biological Transport - drug effects
Bumetanide - pharmacology
Cell physiology
Chlorides - physiology
Diuretics - pharmacology
Erythrocytes - metabolism
Fundamental and applied biological sciences. Psychology
Furosemide - pharmacology
Humans
Membrane and intracellular transports
Molecular and cellular biology
Ouabain - pharmacology
Potassium - metabolism
Sodium - antagonists & inhibitors
Sodium - metabolism
Sodium - pharmacology
Diuretic
Human
Sodium
Membrane transport
Chlorides
Red blood cell
Ions
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Summary:A study has been made of the passive (ouabain-insensitive) Na efflux from human red cells. The inhibition by loop diuretics (furosemide, piretanide and bumetanide) was additive with respect to ouabain and non-additive with respect to each other. They inhibited with high affinity in the sequence bumetanide greater than piretanide greater than furosemide. Passive Na efflux was not inhibited by amiloride, DIDS or SITS. In Cl-free media, Na efflux was reduced and there was no diuretic-sensitive component with any of the six Cl substitutes tested, except Br. The chloride concentration dependence of the diuretic-sensitive efflux was generally linear but saturable with sulphate and concave with acetate. In Na-free media, efflux was reduced, the apparent affinity for loop diuretics was lower and there was no chloride dependence. The diuretic-insensitive efflux was unaffected by anions in all conditions.
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ISSN:0031-6768
1432-2013
DOI:10.1007/BF00640924