Molecular variability of Macrophomina phaseolina isolates using SSR marker

Molecular variability of Macrophomina phaseolina isolates using SSR marker

An experiment was conducted at RARI, Durgapura, SKNAU, Jobner during kharif 2020 to analyze the genetic diversity of the 12 Macrophomina phaseolina isolates by using simple sequence repeat (SSR) markers. The SSR primers were tested for amplification of genomic DNA of Macrophomia phaseolina isolates....

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Bibliographic Details
Published in:The Indian journal of agricultural sciences Vol. 92; no. 1; pp. 123 - 125
Main Authors: PRIYANKA, PRIYANKA, MATHUR, A C, SHARMA, RIDHI SHANKAR, BAGRI, R K, MEENA, A K
Format: Journal Article
Language:English
Published: Indian Council of Agricultural Research 01-01-2022
Subjects:
Clusterbean
Genetic diversity
Macrophomina phaseolina
Molecular variability
SSR markers
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Summary:An experiment was conducted at RARI, Durgapura, SKNAU, Jobner during kharif 2020 to analyze the genetic diversity of the 12 Macrophomina phaseolina isolates by using simple sequence repeat (SSR) markers. The SSR primers were tested for amplification of genomic DNA of Macrophomia phaseolina isolates. Total five primers of MB series were screened against twelve Macrophomia phaseolina isolates. All primes amplified scorable banding pattern. Level of polymorphism for SSR analysis ranged between 60–85.7%. The similarity matrix indicated that 12 isolates exhibited 42.82–100% similarity coefficient. The SSR analysis shows high molecular variability among the isolates grouped into five major clusters A, B, C, D and E. In the similarity matrix Mp-DPR was found to have higher value of similarity coefficient (1), whereas Mp-JSM was found to have lower value if similarity coefficient (0.42).
ISSN:0019-5022
2394-3319
DOI:10.56093/ijas.v92i1.120874