Single sequence repeat markers associated with partial resistance in sunflower to Phoma macdonaldii

Single sequence repeat markers associated with partial resistance in sunflower to Phoma macdonaldii

Phoma black stem of sunflower, caused by Phoma macdonaldii, occurs in many countries. The objective of the present study was to estimate the number of markers and genomic regions in sunflower associating with Phoma black stem resistance. Genetic variability among 32 sunflower genotypes, including re...

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Bibliographic Details
Published in:Phytopathologia mediterranea Vol. 51; no. 3; pp. 541 - 548
Main Authors: DAVAR, Robab, DARVISHZADEH, Reza, REZAEE DANESH, Younes, KHOLGHI, Maryam, AZIZI, Majid, SHAH, Denk A.
Format: Journal Article
Language:English
Published: Florence Mediterranean Phytopathological Union and Firenze University Press 01-12-2012
Firenze University Press Università degli Studi di Firenze
Firenze University Press
Subjects:
association analysis
DNA
Genetic loci
Genotypes
Inbred strains
Irradiation
isolate-nonspecific partial resistance
isolate-specific partial resistance
Medical genetics
phenotype-marker association
Phenotypic traits
Plants
Research Papers
Restriction fragment length polymorphism
simple sequence repeat
Single nucleotide polymorphism
Sunflowers
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Summary:Phoma black stem of sunflower, caused by Phoma macdonaldii, occurs in many countries. The objective of the present study was to estimate the number of markers and genomic regions in sunflower associating with Phoma black stem resistance. Genetic variability among 32 sunflower genotypes, including recombinant inbred lines and their parents, M7 mutant lines developed by gamma irradiation, and some genotypes from different countries of origin, was evaluated using simple sequence repeat (SSR) markers. Eighty-eight markers were generated at 38 SSR loci, with a mean number of alíeles per locus of 2.32. Using susceptibility data of 32 sunflower genotypes against seven P. macdonaldii isolates (Darvishzadeh et al., 2007), one to four markers were associated with each of seven different P. macdonaldii isolates. To reduce the probability of false positives, a sequential Bonferroni-experiment-wise Pvalue was used for each marker trait association tested. The identified markers showed a promising trend, although they did not pass the more stringent bar of statistical significance, and should be studied further.
ISSN:0031-9465
1593-2095
DOI:10.14601/Phytopathol_Mediterr-9868