Search Results - "SEVERINOV, Konstantin"

Systematic prediction of genes functionally linked to CRISPR-Cas systems by gene neighborhood analysis by Shmakov, Sergey A., Makarova, Kira S., Wolf, Yuri I., Severinov, Konstantin V., Koonin, Eugene V.

“…The CRISPR-Cas systems of bacterial and archaeal adaptive immunity consist of direct repeat arrays separated by unique spacers and multiple CRISPR-associated…”
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Multisubunit RNA Polymerases of Jumbo Bacteriophages by Sokolova, Maria L, Misovetc, Inna, V Severinov, Konstantin

“…Prokaryotic viruses with DNA genome longer than 200 kb are collectively referred to as "jumbo phages". Some representatives of this phylogenetically diverse…”
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Mechanism of duplex DNA destabilization by RNA-guided Cas9 nuclease during target interrogation by Mekler, Vladimir, Minakhin, Leonid, Severinov, Konstantin

“…The prokaryotic clustered regularly interspaced short palindromic repeats (CRISPR)-associated 9 (Cas9) endonuclease cleaves double-stranded DNA sequences…”
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Microbial Arsenal of Antiviral Defenses. Part II by Isaev, Artem B., Musharova, Olga S., Severinov, Konstantin V.

“…Bacteriophages or phages are viruses that infect bacterial cells (for the scope of this review we will also consider viruses that infect Archaea). The constant…”
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C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector by Abudayyeh, Omar O., Gootenberg, Jonathan S., Konermann, Silvana, Joung, Julia, Slaymaker, Ian M., Cox, David B. T., Shmakov, Sergey, Makarova, Kira S., Semenova, Ekaterina, Minakhin, Leonid, Severinov, Konstantin, Regev, Aviv, Lander, Eric S., Koonin, Eugene V., Zhang, Feng

“…The clustered regularly interspaced short palindromic repeat (CRISPR)-CRISPR-associated genes (Cas) adaptive immune system defends microbes against foreign…”
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Structure Studies of the CRISPR-Csm Complex Reveal Mechanism of Co-transcriptional Interference by You, Lilan, Ma, Jun, Wang, Jiuyu, Artamonova, Daria, Wang, Min, Liu, Liang, Xiang, Hua, Severinov, Konstantin, Zhang, Xinzheng, Wang, Yanli

“…Csm, a type III-A CRISPR-Cas interference complex, is a CRISPR RNA (crRNA)-guided RNase that also possesses target RNA-dependent DNase and cyclic…”
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Molecular memory of prior infections activates the CRISPR/Cas adaptive bacterial immunity system by Datsenko, Kirill A., Pougach, Ksenia, Tikhonov, Anton, Wanner, Barry L., Severinov, Konstantin, Semenova, Ekaterina

“…CRISPR/Cas (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated genes) is a small RNA-based adaptive prokaryotic immunity system that…”
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The CRISPR Spacer Space Is Dominated by Sequences from Species-Specific Mobilomes by Shmakov, Sergey A, Sitnik, Vassilii, Makarova, Kira S, Wolf, Yuri I, Severinov, Konstantin V, Koonin, Eugene V

“…Clustered regularly interspaced short palindromic repeats and CRISPR-associated protein (CRISPR-Cas) systems store the memory of past encounters with foreign…”
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Kinetics of the CRISPR-Cas9 effector complex assembly and the role of 3'-terminal segment of guide RNA by Mekler, Vladimir, Minakhin, Leonid, Semenova, Ekaterina, Kuznedelov, Konstantin, Severinov, Konstantin

“…CRISPR-Cas9 is widely applied for genome engineering in various organisms. The assembly of single guide RNA (sgRNA) with the Cas9 protein may limit the…”
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Structural basis of template strand deoxyuridine promoter recognition by a viral RNA polymerase by Fraser, Alec, Sokolova, Maria L., Drobysheva, Arina V., Gordeeva, Julia V., Borukhov, Sergei, Jumper, John, Severinov, Konstantin V., Leiman, Petr G.

“…Recognition of promoters in bacterial RNA polymerases (RNAPs) is controlled by sigma subunits. The key sequence motif recognized by the sigma, the −10 promoter…”
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Uncertainty-aware and interpretable evaluation of Cas9–gRNA and Cas12a–gRNA specificity for fully matched and partially mismatched targets with Deep Kernel Learning by Kirillov, Bogdan, Savitskaya, Ekaterina, Panov, Maxim, Ogurtsov, Aleksey Y, Shabalina, Svetlana A, Koonin, Eugene V, Severinov, Konstantin V

“…Abstract The choice of guide RNA (gRNA) for CRISPR-based gene targeting is an essential step in gene editing applications, but the prediction of gRNA…”
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Dependence of post-segregational killing mediated by Type II restriction-modification systems on the lifetime of restriction endonuclease effective activity by Kozlova, Svetlana, Morozova, Natalia, Ispolatov, Yaroslav, Severinov, Konstantin

“…Plasmid-borne Type II restriction-modification (RM) systems mediate post-segregational killing (PSK). PSK is thought to be caused by the dilution of…”
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Optimal number of spacers in CRISPR arrays by Martynov, Alexander, Severinov, Konstantin, Ispolatov, Iaroslav

“…Prokaryotic organisms survive under constant pressure of viruses. CRISPR-Cas system provides its prokaryotic host with an adaptive immune defense against…”
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Microbial Arsenal of Antiviral Defenses – Part I by Isaev, Artem B., Musharova, Olga S., Severinov, Konstantin V.

“…Bacteriophages or phages are viruses that infect bacterial cells (for the scope of this review we will also consider viruses that infect Archaea). Constant…”
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Systematic prediction of functionally linked genes in bacterial and archaeal genomes by Shmakov, Sergey A., Faure, Guilhem, Makarova, Kira S., Wolf, Yuri I., Severinov, Konstantin V., Koonin, Eugene V.

“…Functionally linked genes in bacterial and archaeal genomes are often organized into operons. However, the composition and architecture of operons are highly…”
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Structural Basis of Leader Peptide Recognition in Lasso Peptide Biosynthesis Pathway by Sumida, Tomomi, Dubiley, Svetlana, Wilcox, Brendan, Severinov, Konstantin, Tagami, Shunsuke

“…Lasso peptides are a class of ribosomally synthesized and post-translationally modified peptides (RiPPs) with a unique 3D-interlocked structure, in which an…”
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RNA remodeling and gene regulation by cold shock proteins by Phadtare, Sangita, Severinov, Konstantin

“…One of the many important consequences that temperature down-shift has on cells is stabilization of secondary structures of RNAs. This stabilization has…”
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US sanctions put research at risk by Severinov, Konstantin

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Ultrahigh-throughput functional profiling of microbiota communities by Terekhov, Stanislav S., Smirnov, Ivan V., Malakhova, Maja V., Samoilov, Andrei E., Manolov, Alexander I., Nazarov, Anton S., Danilov, Dmitry V., Dubiley, Svetlana A., Osterman, Ilya A., Rubtsova, Maria P., Kostryukova, Elena S., Ziganshin, Rustam H., Kornienko, Maria A., Vanyushkina, Anna A., Bukato, Olga N., Ilina, Elena N., Vlasov, Valentin V., Severinov, Konstantin V., Gabibov, Alexander G., Altman, Sidney

“…Microbiome spectra serve as critical clues to elucidate the evolutionary biology pathways, potential pathologies, and even behavioral patterns of the host…”
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Single-nucleotide resolution detection of Topo IV cleavage activity in the Escherichia coli genome with Topo-Seq by Sutormin, Dmitry, Galivondzhyan, Alina, Gafurov, Azamat, Severinov, Konstantin

“…Topoisomerase IV (Topo IV) is the main decatenation enzyme in ; it removes catenation links that are formed during DNA replication. Topo IV binding and…”
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