Charm Safe-Level β-Lactam Test for amoxicillin, ampicillin, ceftiofur, cephapirin, and penicillin G in raw commingled milk

Charm Safe-Level β-Lactam Test for amoxicillin, ampicillin, ceftiofur, cephapirin, and penicillin G in raw commingled milk

The Charm Safe-Level beta-Lactam Test was evaluated by a U.S. Food and Drug Administration (FDA) test protocol administered by the AOAC-Research Institute. The sensitivity and selectivity of the test were evaluated with >800 negative raw commingled and drug-fortified milk samples by the manufactu...

Full description

Saved in:
Bibliographic Details
Published in:Journal of AOAC International Vol. 84; no. 1; pp. 29 - 36
Main Authors: SALTER, Robert S, LEGG, David, OSSANNA, Nancy, BOYER, Cheryl, SCHEEMAKER, Joan, MARKOVSKY, Robert, SAUL, Steven J
Format: Journal Article
Language:English
Published: Gaithersburg, MD AOAC International 2001
Subjects:
Amoxicillin - analysis
Ampicillin - analysis
Animals
Anti-Bacterial Agents - analysis
Biological and medical sciences
Cephalosporins - analysis
Cephapirin - analysis
Food industries
Fundamental and applied biological sciences. Psychology
Indicators and Reagents
Milk - chemistry
Milk - microbiology
Milk and cheese industries. Ice creams
Penicillin G - analysis
Quality Control
β-Lactams
Dairy product
Bulk
Milk production
Antimicrobial agent
Certification
Raw milk
Antibiotic
Residue
Quality control
Kit
Control method
Food and Drug Administration
Chemical contamination
Visual control
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The Charm Safe-Level beta-Lactam Test was evaluated by a U.S. Food and Drug Administration (FDA) test protocol administered by the AOAC-Research Institute. The sensitivity and selectivity of the test were evaluated with >800 negative raw commingled and drug-fortified milk samples by the manufacturer and an independent laboratory. Probit analysis by the independent laboratory determined the following 90% positive levels with 95% confidence: amoxicillin, 5.6 ppb; ampicillin, 8.5 ppb; cephapirin, 13.7 ppb; ceftiofur, 46.2 ppb; and penicillin G, 3.6 ppb. These values were within a range of +/- 20% of the manufacturer's data. Selection of negative samples met confidence specifications. Ruggedness parameters were studied and defined, and the stability of frozen milk was verified. There were no interferences from somatic cells (1,000,000 somatic cell count/mL) or bacteria (300,000 colony-forming units/mL), or from 27 other non-beta-lactam animal drugs. Test performance with raw milk samples containing incurred penicillin, ampicillin, and amoxicillin was consistent with the dose responses determined with fortified milk samples. Incurred cephalosporin in raw milk samples was detected at lower levels than was cephalosporin in fortified milk samples, presumably because of the presence of metabolite, as verified by other test methods. Quality control data support consistency in manufacture between batches and the stability of refrigerated test reagents for up to 1 year. Successful fulfillment of these criteria led to FDA certification of the test when used with a reader in U.S. milk testing programs.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1060-3271
1944-7922
DOI:10.1093/jaoac/84.1.29