Search Results - "S. Silva, Ricardo J."

Extending AAV Packaging Cargo through Dual Co-Transduction: Efficient Protein Trans-Splicing at Low Vector Doses by Ferreira, Mariana V, Fernandes, Sofia, Almeida, Ana Isabel, Neto, Salomé, Mendes, João P, Silva, Ricardo J S, Peixoto, Cristina, Coroadinha, Ana Sofia

“…Adeno-associated viral (AAV) vectors represent one of the leading platforms for gene delivery. Nevertheless, their small packaging capacity restricts their use…”
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Assessing Multi-Attribute Characterization of Enveloped and Non-Enveloped Viral Particles by Capillary Electrophoresis by Fernandes, Rita P, Escandell, José M, Guerreiro, Ana C L, Moura, Filipa, Faria, Tiago Q, Carvalho, Sofia B, Silva, Ricardo J S, Gomes-Alves, Patrícia, Peixoto, Cristina

“…Virus-based biopharmaceutical products are used in clinical applications such as vaccines, gene therapy, and immunotherapy. However, their manufacturing…”
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Adenovirus purification by two-column, size-exclusion, simulated countercurrent chromatography by Nestola, Piergiuseppe, Silva, Ricardo J.S., Peixoto, Cristina, Alves, Paula M., Carrondo, Manuel J.T., Mota, José P.B.

“…•Ad5 was purified by size-exclusion chromatography (SEC) in a 2-column open-loop SMB system.•Clearance of impurities, namely DNA and host cell protein (HCP),…”
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Accelerated Development of AAV Purification Process Using a High-Throughput and Automated Crossflow System by Mendes, Sónia, Faria, Tiago Q., Nascimento, André, Noverraz, Marc, Bollmann, Franziska, Nestola, Piergiuseppe, Roldão, António, Peixoto, Cristina, Silva, Ricardo J. S.

“…Adeno-associated viruses (AAV) are currently predominant viral transfer tools for gene therapy, and efforts are being made to design faster and more efficient…”
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Improved virus purification processes for vaccines and gene therapy by Nestola, Piergiuseppe, Peixoto, Cristina, Silva, Ricardo R. J. S, Alves, Paula M., Mota, José P. B., Carrondo, Manuel J. T.

“…ABSTRACT The downstream processing of virus particles for vaccination or gene therapy is becoming a critical bottleneck as upstream titers keep improving…”
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Downstream processing for influenza vaccines and candidates: An update by B. Carvalho, Sofia, Peixoto, Cristina, T. Carrondo, Manuel J., S. Silva, Ricardo J.

“…Seasonal and pandemic influenza outbreaks present severe health and economic burdens. To overcome limitations on influenza vaccines' availability and…”
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A new multicolumn, open-loop process for center-cut separation by solvent-gradient chromatography by Silva, Ricardo J.S., Rodrigues, Rui C.R., Osuna-Sanchez, Hector, Bailly, Michel, Valéry, Eric, Mota, José P.B.

“…A comprehensive description of a new process—the GSSR (Gradient with Steady State Recycle) process—for center-cut separation by solvent-gradient chromatography…”
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Streamlined, two-column, simulated countercurrent chromatography for binary separation by Rodrigues, Rui C.R., Silva, Ricardo J.S., Mota, José P.B.

“…We report on a numerical and experimental study of two-column versions of streamlined, multicolumn, semi-continuous chromatography for binary separation. The…”
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Continuous Affinity Purification of Adeno-Associated Virus Using Periodic Counter-Current Chromatography by Mendes, João P, Bergman, Magnus, Solbrand, Anita, Peixoto, Cristina, Carrondo, Manuel J T, Silva, Ricardo J S

“…Replacing batch unit operations of biopharmaceuticals by continuous manufacturing is a maturing concept, with periodic counter-current chromatography (PCC)…”
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AAV process intensification by perfusion bioreaction and integrated clarification by Mendes, João P., Fernandes, Bárbara, Pineda, Earl, Kudugunti, Shashi, Bransby, Mike, Gantier, René, Peixoto, Cristina, Alves, Paula M., Roldão, António, Silva, Ricardo J. S.

“…Adeno-associated viruses (AAVs) demand for clinical trials and approved therapeutic applications is increasing due to this vector’s overall success and…”
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Exploring continuous and integrated strategies for the up- and downstream processing of human mesenchymal stem cells by Cunha, Bárbara, Aguiar, Tiago, Silva, Marta M., Silva, Ricardo J.S., Sousa, Marcos F.Q., Pineda, Earl, Peixoto, Cristina, Carrondo, Manuel J.T., Serra, Margarida, Alves, Paula M.

“…•Establishment of an integrated strategy for up- and downstream processing of hMSC.•Continuous perfusion culture increased hMSC expansion and led to a…”
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Robust design of adenovirus purification by two-column, simulated moving-bed, size-exclusion chromatography by Nestola, Piergiuseppe, Silva, Ricardo J.S., Peixoto, Cristina, Alves, Paula M., Carrondo, Manuel J.T., Mota, José P.B.

“…A simple, yet efficient, two-column simulated moving-bed (2CSMB) process for purifying adenovirus serotype 5 (Ad5) by size-exclusion chromatography (SEC) is…”
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Efficient adeno-associated virus serotype 5 capture with affinity functionalized nanofiber adsorbents by Neto, Salomé, Mendes, João P, Santos, Susana B Dos, Solbrand, Anita, Carrondo, Manuel J T, Peixoto, Cristina, Silva, Ricardo J S

“…Adeno-associated viruses (AAVs) are one of the most promising tools for gene therapy applications. These vectors are purified using affinity and ion exchange…”
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Bioanalytics for Influenza Virus-Like Particle Characterization and Process Monitoring by Carvalho, Sofia B, Silva, Ricardo J S, Sousa, Marcos F Q, Peixoto, Cristina, Roldão, António, Carrondo, Manuel J T, Alves, Paula M

“…Virus-like particles (VLPs) are excellent platforms for the development of influenza vaccine candidates. Nonetheless, their characterization is challenging due…”
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Improving washing strategies of human mesenchymal stem cells using negative mode expanded bed chromatography by Cunha, Bárbara, Silva, Ricardo J.S., Aguiar, Tiago, Serra, Margarida, Daicic, John, Maloisel, Jean-Luc, Clachan, John, Åkerblom, Anna, Carrondo, Manuel J.T., Peixoto, Cristina, Alves, Paula M.

“…•Expanded bed chromatography (EBA) was used to improve washing efficiency of hMSC.•A new multimodal matrix based on core bead technology was used in negative…”
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Development, Construction, and Operation of a Multisample Volumetric Apparatus for the Study of Gas Adsorption Equilibrium by Ribeiro, Rui P. P. L, Silva, Ricardo J. S, Esteves, Isabel A. A. C, Mota, José P. B

“…The construction of a simple volumetric adsorption apparatus is highlighted. The setup is inexpensive and provides a clear demonstration of gas phase…”
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Current challenges in biotherapeutic particles manufacturing by Moleirinho, Mafalda G, Silva, Ricardo J S, Alves, Paula M, Carrondo, Manuel J T, Peixoto, Cristina

“…: The development of novel complex biotherapeutics led to new challenges in biopharmaceutical industry. The potential of these particles has been demonstrated…”
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Relay simulated moving bed chromatography: Concept and design criteria by Silva, Ricardo J.S., Rodrigues, Rui C.R., Mota, José P.B.

“…► The R-SMB is a new implementation of SMB chromatography with intermittent or pulsed outlets. ► The R-SMB is an analog of the SMB in terms of displaced…”
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Towards a scalable bioprocess for rAAV production using a HeLa stable cell line by Escandell, José, Moura, Filipa, Carvalho, Sofia B., Silva, Ricardo J. S., Correia, Ricardo, Roldão, Antonio, Gomes‐Alves, Patrícia, Alves, Paula M.

“…The majority of recombinant adeno‐associated viruses (rAAV) approved for clinical use or in clinical trials areproduced by transient transfection using the…”
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Development of versatile affinity‐based system for one step purification process: Case of Group A Streptococcus vaccine by Chevrel, Anne, Candela, Leo, Innocenti, Elisa, Golibrzuch, Carolin, Skudas, Romas, Schwämmle, Achim, Carrondo, Manuel J. T., Kitten, Olivier, Nissum, Mikkel, Silva, Ricardo J. S.

“…Affinity capture is one of the most attractive strategies for simplifying downstream processing. Although it is a key mainstream approach for antibody…”
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