Characterization of Silver Nanoparticles Obtained by a Green Route and Their Evaluation in the Bacterium of Pseudomonas aeruginosa

Characterization of Silver Nanoparticles Obtained by a Green Route and Their Evaluation in the Bacterium of Pseudomonas aeruginosa

Metal nanoparticles are widely used in different areas such as biotechnology and biomedicine, for example in drug delivery, imaging and control of bacterial growth. The antimicrobial effect of silver has been identified as an alternative approach to the increasing bacterial resistance to antibiotics...

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Bibliographic Details
Published in:Crystals (Basel) Vol. 10; no. 5; p. 395
Main Authors: Martínez Espinosa, Juan Carlos, Carrera Cerritos, Raúl, Ramírez Morales, Maria Antonieta, Sánchez Guerrero, Karla Paola, Silva Contreras, Rocio Alejandra, Macías, Juan H.
Format: Journal Article
Language:English
Published: Basel MDPI AG 01-05-2020
Subjects:
antibacterial activity
Antibacterial agents
Antibiotics
Antiinfectives and antibacterials
Antimicrobial agents
Bacteria
Chemical synthesis
Composition
Diameters
Diffraction patterns
Drug delivery systems
Electron diffraction
Evaluation
Green chemistry
green synthesis
Health aspects
Laboratories
Methods
Microorganisms
Morphology
Nanoparticles
Photoelectrons
Properties
Pseudomonas aeruginosa
Reducing agents
Silver
silver nanoparticles
Spectrophotometry
Transmission electron microscopy
X ray photoelectron spectroscopy
Mexico
United States > US
Japan
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Summary:Metal nanoparticles are widely used in different areas such as biotechnology and biomedicine, for example in drug delivery, imaging and control of bacterial growth. The antimicrobial effect of silver has been identified as an alternative approach to the increasing bacterial resistance to antibiotics. Silver nanoparticles were synthesized by the green route using the Geranium extract as a reducing agent. The characterization was carried out by the techniques of UV-Vis spectrophotometry, transmission electron microscopy (TEM), high-resolution transmission electron microscopy (HRTEM), X-ray emitted photoelectron spectroscopy (XPS) and X-ray diffraction. Nanoparticle diameters between 15 and 50 nm were obtained and the interplanar spaces calculated from the electron diffraction pattern corresponding to a mixture of silver with 4H and FCC structures. To determine the minimum inhibitory concentration of silver nanoparticles (AgNPs) on the Pseudomonas aeruginosa bacteria (ATCC-27853), different concentrations of colloidal solution 0.36, 0.18, 0.09 and 0.05 μg/mL were evaluated as a function of the incubation time, measuring the inhibition halo and colony forming unit (CFU) during 0, 2 and 4 h of incubation. The minimum inhibitory AgNPs concentration (MIC) is 0.36 μg/mL at 0 h while the concentration of 0.18 μg/mL presents a total inhibition of the bacterium after 2 h. For the rest of the dilutions, gradual inhibitions as a function of time were observed. We evaluate the antibacterial effect of silver nanoparticles obtained by a green methodology in Pseudomonas aeruginosa bacteria. Finally, the colloidal nanoparticle solution can be an antibacterial alternative for different biomedical approaches.
ISSN:2073-4352
2073-4352
DOI:10.3390/cryst10050395