Reproducibility of a hemi mandible positioning device and a method for measuring alveolar bone loss area in mice

The objective of this study was to determine the reproducibility of a positioning device and a method for measuring the area of alveolar bone loss (ABL) in hemi mandibles of mice. Mucoperiosteal flaps were raised in CF1 Mus domesticus mice (n = 10) on the buccal aspects of the left side of the mandi...

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Bibliographic Details
Published in:	Journal of Oral Science Vol. 49; no. 1; pp. 13 - 17
Main Authors:	Rivaldo, Elken G., Padilha, Dalva M. P., Hugo, Fernando N., Hilgert, Juliana B., Rybu, Bruno R.
Format:	Journal Article
Language:	English
Published:	Japan Nihon University School of Dentistry 2007
Subjects:	alveolar bone loss Alveolar Bone Loss - diagnosis Animals Dentistry Equipment Design Male Mandible Mice Photography, Dental Reproducibility of Results result reproducibility
Online Access:	Get full text
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Summary:	The objective of this study was to determine the reproducibility of a positioning device and a method for measuring the area of alveolar bone loss (ABL) in hemi mandibles of mice. Mucoperiosteal flaps were raised in CF1 Mus domesticus mice (n = 10) on the buccal aspects of the left side of the mandibles. Twenty-one days after surgery, the animals were sacrificed, and the mandibles were resected and stained with 1% toluidine blue. Two positioning devices, one for the buccal and the other for lingual aspect, were fabricated to keep the hemi mandibles in a standard position. The reliabilities of the positioning device and the method for measuring ABL area were analyzed using two series of pictures. The photographs were digitized and ABL measured as the exposed root area. Measurements were performed by two blinded examiners using image analysis software. Intra- and inter-examiner reproducibility of the positioning method ranged from -1.5 to +1.33 mm, while intra- and inter-examiner reproducibility of the measurement technique ranged from -3.37 to 14.70. Our results indicate that, due to the small variation observed in ABL area assessment, the positioning device and method for measuring ABL area represent useful techniques for evaluating ABL in mice. (J. Oral Sci. 49, 13-17, 2007)
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1343-4934 1880-4926
DOI:	10.2334/josnusd.49.13