Search Results - "Russo, Christopher J"

Measuring the effects of particle orientation to improve the efficiency of electron cryomicroscopy by Naydenova, Katerina, Russo, Christopher J.

“…The orientation distribution of a single-particle electron cryomicroscopy specimen limits the resolution of the reconstructed density map. Here we define a…”
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Ultrastable gold substrates for electron cryomicroscopy by Russo, Christopher J., Passmore, Lori A.

“…Despite recent advances, the structures of many proteins cannot be determined by electron cryomicroscopy because the individual proteins move during…”
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Multifunctional graphene supports for electron cryomicroscopy by Naydenova, Katerina, Peet, Mathew J., Russo, Christopher J.

“…With recent technological advances, the atomic resolution structure of any purified biomolecular complex can, in principle, be determined by single-particle…”
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Controlling protein adsorption on graphene for cryo-EM using low-energy hydrogen plasmas by Russo, Christopher J, Passmore, Lori A

“…Graphene is in many ways an ideal sample support for cryo-electron microscopy, but its hydrophobicity prevents adsorption of protein from aqueous solution…”
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Ewald sphere correction using a single side-band image processing algorithm by Russo, Christopher J., Henderson, Richard

“…•A method for correcting for the curvature of the Ewald sphere is presented.•We demonstrate the algorithm using gold nanoparticles embedded in ice.•The…”
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The energy dependence of contrast and damage in electron cryomicroscopy of biological molecules by Peet, Mathew J., Henderson, Richard, Russo, Christopher J.

“…•Carbon elastic and inelastic electron scattering cross sections are measured vs. energy.•Elastic scattering is compared to energy deposition and radiation…”
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Atom-by-atom nucleation and growth of graphene nanopores by Russo, Christopher J, Golovchenko, J. A

“…Graphene is an ideal thin membrane substrate for creating molecule-scale devices. Here we demonstrate a scalable method for creating extremely small structures…”
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Charge accumulation in electron cryomicroscopy by Russo, Christopher J., Henderson, Richard

“…•A physical account of charge accumulation in low-dose cryoEM is presented.•We describe electrostatic micro-lenses that are extremely sensitive charge…”
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Progress towards an optimal specimen support for electron cryomicroscopy by Russo, Christopher J, Passmore, Lori A

“…[Display omitted] •Physical principles of electron scattering govern the design of specimen supports.•Radiation-induced motion causes loss of resolution in…”
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Structure of the SARS-CoV-2 RNA-dependent RNA polymerase in the presence of favipiravir-RTP by Naydenova, Katerina, Muir, Kyle W., Wu, Long-Fei, Zhang, Ziguo, Coscia, Francesca, Peet, Mathew J., Castro-Hartmann, Pablo, Qian, Pu, Sader, Kasim, Dent, Kyle, Kimanius, Dari, Sutherland, John D., Löwe, Jan, Barford, David, Russo, Christopher J.

“…The RNA polymerase inhibitor favipiravir is currently in clinical trials as a treatment for infection with severe acute respiratory syndrome coronavirus 2…”
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Advances in Single-Particle Electron Cryomicroscopy Structure Determination applied to Sub-tomogram Averaging by Bharat, Tanmay A.M., Russo, Christopher J., Löwe, Jan, Passmore, Lori A., Scheres, Sjors H.W.

“…Recent innovations in specimen preparation, data collection, and image processing have led to improved structure determination using single-particle electron…”
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On the reduction in the effects of radiation damage to two-dimensional crystals of organic and biological molecules at liquid-helium temperature by Naydenova, Katerina, Kamegawa, Akiko, Peet, Mathew J., Henderson, Richard, Fujiyoshi, Yoshinori, Russo, Christopher J.

“…We have studied the fading of electron diffraction spots from two-dimensional (2D) crystals of paraffin (C44H90), purple membrane (bacteriorhodopsin) and…”
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Impact of brain tissue filtering on neurostimulation fields: A modeling study by Wagner, Tim, Eden, Uri, Rushmore, Jarrett, Russo, Christopher J., Dipietro, Laura, Fregni, Felipe, Simon, Stephen, Rotman, Stephen, Pitskel, Naomi B., Ramos-Estebanez, Ciro, Pascual-Leone, Alvaro, Grodzinsky, Alan J., Zahn, Markus, Valero-Cabré, Antoni

“…Electrical neurostimulation techniques, such as deep brain stimulation (DBS) and transcranial magnetic stimulation (TMS), are increasingly used in the…”
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Cryo-EM with sub-1 Å specimen movement by Naydenova, Katerina, Jia, Peipei, Russo, Christopher J

“…Most information loss in cryogenic electron microscopy (cryo-EM) stems from particle movement during imaging, which remains poorly understood. We show that…”
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The 2.4 Å cryo-EM structure of a heptameric light-harvesting 2 complex reveals two carotenoid energy transfer pathways by Gardiner, Alastair T, Naydenova, Katerina, Castro-Hartmann, Pablo, Nguyen-Phan, Tu C, Russo, Christopher J, Sader, Kasim, Hunter, C Neil, Cogdell, Richard J, Qian, Pu

“…We report the 2.4 Ångström resolution structure of the light-harvesting 2 (LH2) complex from ( ) determined by cryogenic electron microscopy. The structure…”
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Integrated wafer-scale manufacturing of electron cryomicroscopy specimen supports by Naydenova, Katerina, Russo, Christopher J.

“…We present a process for the manufacture of electron cryomicroscopy (cryoEM) specimen supports with an integrated foil–grid structure, using cryogenic vacuum…”
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Microscopic charge fluctuations cause minimal contrast loss in cryoEM by Russo, Christopher J., Henderson, Richard

“…•A physical account of charge fluctuations in low-dose cryoEM is presented.•We quantify the bee swarm charging phenomenon on cryoEM specimen.•We measure the…”
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Ultrastable gold substrates: Properties of a support for high-resolution electron cryomicroscopy of biological specimens by Russo, Christopher J., Passmore, Lori A.

“…Electron cryomicroscopy (cryo-EM) allows structure determination of a wide range of biological molecules and specimens. All-gold supports improve cryo-EM…”
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Honeycomb gold specimen supports enabling orthogonal focussed ion beam-milling of elongated cells for cryo-ET by Hale, Victoria L., Hooker, James, Russo, Christopher J., Löwe, Jan

“…[Display omitted] •We present a method that allows the cryo-EM/ET (electron cryo-microscopy/tomography) imaging of rod-shaped bacteria orthogonal to their…”
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Cryomicroscopy in situ : what is the smallest molecule that can be directly identified without labels in a cell? by Russo, Christopher J, Dickerson, Joshua L, Naydenova, Katerina

“…Electron cryomicroscopy (cryoEM) has made great strides in the last decade, such that the atomic structure of most biological macromolecules can, at least in…”
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