Search Results - "Runquist, Jennifer A."

Functional contribution of a conserved, mobile loop histidine of phosphoribulokinase by Runquist, Jennifer A., Miziorko, Henry M.

“…In the Rhodobacter sphaeroides phosphoribulokinase (PRK) structure, there are several disordered regions, including a loop containing invariant residues Y98…”
Get full text

Functional Insights into Human HMG-CoA Lyase from Structures of Acyl-CoA-containing Ternary Complexes by Fu, Zhuji, Runquist, Jennifer A., Montgomery, Christa, Miziorko, Henry M., Kim, Jung-Ja P.

“…HMG-CoA lyase (HMGCL) is crucial to ketogenesis, and inherited human mutations are potentially lethal. Detailed understanding of the HMGCL reaction mechanism…”
Get full text

Functional Evaluation of Serine/Threonine Residues in the P-Loop of Rhodobacter sphaeroides Phosphoribulokinase by Runquist, Jennifer A, Ríos, Sandra E, Vinarov, Dmitriy A, Miziorko, Henry M

“…The N-terminal region of phosphoribulokinase (PRK) has been proposed to contain a “P-loop” or “Walker A” motif. In Rhodobacter sphaeroides PRK, four alcohol…”
Get full text

Identification of the Allosteric Regulatory Site in Bacterial Phosphoribulokinase by Kung, Guosheng, Runquist, Jennifer A, Miziorko, Henry M, Harrison, David H. T

“…Bacterial phosphoribulokinases (PRKs) are octameric members of the adenylate kinase family of enzymes. The enzyme is allosterically activated by NADH and…”
Get full text

Rhodobacter sphaeroides Phosphoribulokinase:  Identification of Lysine-165 as a Catalytic Residue and Evaluation of the Contributions of Invariant Basic Amino Acids to Ribulose 5-Phosphate Binding by Runquist, Jennifer A, Harrison, David H. T, Miziorko, Henry M

“…Rhodobacter sphaeroides phosphoribulokinase (PRK) is inactivated upon exposure to pyridoxal phosphate/sodium borohydride, suggesting a reactive lysine residue…”
Get full text

Functional Evaluation of Invariant Arginines Situated in the Mobile Lid Domain of Phosphoribulokinase by Runquist, Jennifer A, Harrison, David H. T, Miziorko, Henry M

“…Rhodobacter sphaeroides phosphoribulokinase contains four invariant arginines (R49, R168, R173, and R187). The high-resolution structure of this enzyme…”
Get full text

Crystal Structure of Human 3-Hydroxy-3-methylglutaryl-CoA Lyase: INSIGHTS INTO CATALYSIS AND THE MOLECULAR BASIS FOR HYDROXYMETHYLGLUTARIC ACIDURIA by Fu, Zhuji, Runquist, Jennifer A, Forouhar, Farhad, Hussain, Munif, Hunt, John F, Miziorko, Henry M, Kim, Jung-Ja P

“…3-Hydroxy-3-methylglutaryl-CoA (HMG-CoA) lyase is a key enzyme in the ketogenic pathway that supplies metabolic fuel to extrahepatic tissues. Enzyme deficiency…”
Get full text

Crystal Structure of Human 3-Hydroxy-3-methylglutaryl-CoA Lyase by Fu, Zhuji, Runquist, Jennifer A., Forouhar, Farhad, Hussain, Munif, Hunt, John F., Miziorko, Henry M., Kim, Jung-Ja P.

“…3-Hydroxy-3-methylglutaryl-CoA (HMG-CoA) lyase is a key enzyme in the ketogenic pathway that supplies metabolic fuel to extrahepatic tissues. Enzyme deficiency…”
Get full text

Identification of the phosphoribulokinase sugar phosphate binding domain by Sandbaken, Mark G, Runquist, Jennifer A, Barbieri, Joseph T, Miziorko, Henry M

“…A recombinant form of Rhodobacter sphaeroides phosphoribulokinase (form I; NADH dependent) has been expressed in and purified to homogeneity from Escherichia…”
Get full text

Anionic substitutes for catalytic aspartic acids in phosphoribulokinase by Runquist, Jennifer A, Miziorko, Henry M

“…Mutagenic substitution of the invariant D42 and D169 residues in phosphoribulokinase (PRK) with amino acids that contain neutral side chains (e.g., alanine or…”
Get full text

Rhodobacter sphaeroides Phosphoribulokinase:  Binary and Ternary Complexes with Nucleotide Substrate Analogs and Effectors by Runquist, Jennifer A, Narasimhan, Chakravarthy, Wolff, Carl E, Koteiche, Hanane A, Miziorko, Henry M

“…Rhodobacter sphaeroides phosphoribulokinase (PRK) binds ATP substrate, as well as spectroscopically active ATP analogs (trinitrophenyl-ATP and…”
Get full text

The Crystal Structure of Phosphoribulokinase from Rhodobacter s phaeroides Reveals a Fold Similar to That of Adenylate Kinase by Harrison, David H. T, Runquist, Jennifer A, Holub, Alison, Miziorko, Henry M

“…The essential photosynthetic enzyme phosphoribulokinase (PRK) is responsible for the conversion of ribulose 5-phosphate (Ru5P) to ribulose 1,5-bisphosphate,…”
Get full text

Evidence Supporting Catalytic Roles for Aspartate Residues in Phosphoribulokinase by Charlier, Henry A, Runquist, Jennifer A, Miziorko, Henry M

“…The DNA encoding Rhodobacter sphaeroides phosphoribulokinase (PRK) has been modified to allow ligation into pET-3d. Using the resulting expression plasmid, PRK…”
Get full text

Utility of a Novel Spin-Labeled Nucleotide in Investigation of the Substrate and Effector Sites of Phosphoribulokinase by Koteiche, Hanane A, Narasimhan, Chakravarthy, Runquist, Jennifer A, Miziorko, Henry M

“…The activated spin-label 3-(2-bromoacetamido)proxyl modifies the sulfur atom of phosphorothioate-containing AMP, ADP, and ATP analogs in a facile reaction that…”
Get full text

Functional contribution of a conserved, mobile loop histidine of phosphoribulokinase by Runquist, Jennifer A., Miziorko, Henry M.

“…In the Rhodobacter sphaeroides phosphoribulokinase (PRK) structure, there are several disordered regions, including a loop containing invariant residues Y98…”
Get full text

Functional contribution of a conserved, mobile loop histidine of phosphoribulokinase by Runquist, Jennifer A., Miziorko, Henry M.

“…In the Rhodobacter sphaeroides phosphoribulokinase (PRK) structure, there are several disordered regions, including a loop containing invariant residues Y98…”
Get full text

Crystal structure of a human hydroxymethyglutaryl‐CoA lyase (HMGCL) complex with inhibitor and divalent cation suggests the basis for substrate stereospecificity by Miziorko, Henry M, Fu, Zhuji, Runquist, Jennifer A, Kim, Jung‐Ja P

“…3‐(R,S)‐Hydroxyglutaryl‐CoA (HG‐CoA) has been soaked into HMGCL crystals, supporting determination of a structure (2.4 Angstrom) for the ternary…”
Get full text

Catalysis of electron transfer across phospholipid bilayers by iron-porphyrin complexes by Runquist, J A, Loach, P A

“…Phospholipid vesicles containing K3Fe(CN)6 were prepared form egg yolk phosphatidylcholine. Hemin dimethyl ester was incorporated into these vesicles during…”
Get more information

The Crystal Structure of Phosphoribulokinase from Rhodobacter sphaeroides Reveals a Fold Similar to That of Adenylate Kinase by HARRISON, David H. T., RUNQUIST, Jennifer A., HOLUB, Alison, MIZIORKO, Henry M.

“…The essential photosynthetic enzyme phosphoribulokinase (PRK) is responsible for the conversion of ribulose 5-phosphate (Ru5P) to ribulose 1,5-bisphosphate,…”
Get full text

The primary structure of the subunits of carbon monoxide dehydrogenase/acetyl-CoA synthase from Clostridium thermoaceticum by MORTON, T. A, RUNQUIST, J. A, RAGSDALE, S. W, SHANMUGASUNDARAM, T, WOOD, H. G, LJUNGDAHL, L. G

“…CO dehydrogenase/acetyl-coenzyme A synthase (CODH) is the central enzyme in the pathway of acetyl-coenzyme A biosynthesis in Clostridium thermoaceticum. It…”
Get full text