Search Results - "Rule, Gordon S."

Specific Labeling of Threonine Methyl Groups for NMR Studies of Protein–Nucleic Acid Complexes by Sinha, Kaustubh, Jen-Jacobson, Linda, Rule, Gordon S

“…Specific 13C labeling of Thr methyl groups has been accomplished via the growth of a standard laboratory strain of Escherichia coli on [2-13C]glycerol in the…”
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Stabilization of Active Site Dynamics Leads to Increased Activity with 3′-Azido-3′-deoxythymidine Monophosphate for F105Y Mutant Human Thymidylate Kinase by Fucci, Ian J, Sinha, Kaustubh, Rule, Gordon S

“…Thymidylate kinases are essential enzymes with roles in DNA synthesis and repair and have been the target of drug development for antimalarials, antifungals,…”
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Labeling of methyl groups: a streamlined protocol and guidance for the selection of 2H precursors based on molecular weight by Locke, Alexandra, Guarino, Kylee, Rule, Gordon S.

“…A streamlined one-day protocol is described to produce isotopically methyl-labeled protein with high levels of deuterium for NMR studies. Using this protocol,…”
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Double Histidine Based EPR Measurements at Physiological Temperatures Permit Site‐Specific Elucidation of Hidden Dynamics in Enzymes by Singewald, Kevin, Bogetti, Xiaowei, Sinha, Kaustubh, Rule, Gordon S, Saxena, Sunil

“…Protein dynamics is at the heart of all cellular processes. Here, we utilize the dHis‐CuIINTA label to obtain site‐specific information on dynamics for both an…”
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Conformational diversity defines substrate specificity of thymidylate/uridylate kinase from Candida albicans by Sinha, Kaustubh, Rule, Gordon S

“…Thymidylate kinase (TMK) from Candida albicans (CaTMK) contains a unique 15 residue insert, the CaLoop, that is not found on other TMKs. CaTMK is proficient at…”
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The Cu2+-nitrilotriacetic acid complex improves loading of α-helical double histidine site for precise distance measurements by pulsed ESR by Ghosh, Shreya, Lawless, Matthew J., Rule, Gordon S., Saxena, Sunil

“…[Display omitted] •Cu2+-NTA complex binds to α-helical dHis sites with greater affinity than Cu2+-IDA.•Signal increases 2-fold for inter-α-helix distances…”
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Rational Control of Protein–Protein Interactions with Protein-ATRP-Generated Protease-Sensitive Polymer Cages by Kaupbayeva, Bibifatima, Murata, Hironobu, Rule, Gordon S., Matyjaszewski, Krzysztof, Russell, Alan J.

“…Protease–protease interactions lie at the heart of the biological cascades that provide rapid molecular responses to living systems. Blood clotting cascades,…”
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The Structure of Thymidylate Kinase from Candida albicans Reveals a Unique Structural Element by Sinha, Kaustubh, Rule, Gordon S

“…The structure of thymidylate kinase from Candida albicans, determined by X-ray crystallography, is reported to a resolution of 2.45 Å with a final R free of…”
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Protein Dynamics Is Altered by a High Surface Density of Atomic Transfer Radical Polymerization Polymers by Fucci, Ian J, Sinha, Kaustubh, Rule, Gordon S

“…The effect of atomic transfer radical polymerization (ATRP) polymers on the structure and dynamics of a 14.5 kDa RNA binding protein, Rho130, was assessed…”
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ESR Resolves the C Terminus Structure of the Ligand-free Human Glutathione S-Transferase A1-1 by Lawless, Matthew J., Pettersson, John R., Rule, Gordon S., Lanni, Frederick, Saxena, Sunil

“…Nitroxide- and Cu2+-based electron spin resonance (ESR) are combined to provide insight into the conformational states of the functionally important α-helix of…”
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dGMP Binding to Thymidylate Kinase from Plasmodium falciparum Shows Half-Site Binding and Induces Protein Dynamics at the Dimer Interface by Chen, Mengshen David, Fucci, Ian J, Sinha, Kaustubh, Rule, Gordon S

“…Plasmodium falciparum thymidylate kinase (PfTMK) is an essential enzyme for the growth of the organism because of its critical role in the de novo synthesis of…”
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Dual Lifetimes for Complexes between Glutathione‑S‑transferase (hGSTA1-1) and Product-like Ligands Detected by Single-Molecule Fluorescence Imaging by Pettersson, John R, Lanni, Frederick, Rule, Gordon S

“…Single-molecule fluorescence techniques were used to characterize the binding of products and inhibitors to human glutathione S-transferase A1-1 (hGSTA1-1)…”
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Interaction of α‑Thymidine Inhibitors with Thymidylate Kinase from Plasmodium falciparum by Chen, Mengshen “David”, Sinha, Kaustubh, Rule, Gordon S, Ly, Danith H

“…Plasmodium falciparum thymidylate kinase (PfTMK) is a critical enzyme in the de novo biosynthesis pathway of pyrimidine nucleotides…”
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Design of Bivalent Nucleic Acid Ligands for Recognition of RNA-Repeated Expansion Associated with Huntington’s Disease by Thadke, Shivaji A, Perera, J. Dinithi R, Hridya, V. M, Bhatt, Kirti, Shaikh, Ashif Y, Hsieh, Wei-Che, Chen, Mengshen, Gayathri, Chakicherla, Gil, Roberto R, Rule, Gordon S, Mukherjee, Arnab, Thornton, Charles A, Ly, Danith H

“…We report the development of a new class of nucleic acid ligands that is comprised of Janus bases and the MPγPNA backbone and is capable of binding rCAG…”
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Metal Ion Binding at the Catalytic Site Induces Widely Distributed Changes in a Sequence Specific Protein–DNA Complex by Sinha, Kaustubh, Sangani, Sahil S, Kehr, Andrew D, Rule, Gordon S, Jen-Jacobson, Linda

“…Metal ion cofactors can alter the energetics and specificity of sequence specific protein–DNA interactions, but it is unknown if the underlying effects on…”
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Sequence-specific Interactions in the RNA-binding Domain of Escherichia coli Transcription Termination Factor Rho by Hitchens, T. Kevin, Zhan, Yiping, Richardson, Lislott V., Richardson, John P., Rule, Gordon S.

“…Rho factor is an essential protein that causes termination of transcription in a wide variety of bacteria by an RNA-dependent helicase activity. Rho is…”
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Stable Triplet of Uracil−Uracil Basepairs in a Small Antisense RNA by Zhan, Yiping, Rule, Gordon S

“…The interaction between ant (antirepressor) mRNA and its antisense RNA, sar (small antisense RNA), is important in regulation of the development of…”
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Tertiary Structure of the Major House Dust Mite Allergen Der p 2:  Sequential and Structural Homologies by Mueller, Geoffrey A, Benjamin, David C, Rule, Gordon S

“…Sensitization to indoor allergens, especially those of the house dust mite, is strongly correlated with the development of asthma. We report the tertiary…”
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Divide and conquer is always best: sensitivity of methyl correlation experiments by Sinha, Kaustubh, Jen-Jacobson, Linda, Rule, Gordon S.

“…The HMCM [CG]CBCA experiment (Tugarinov and Kay in J Am Chem Soc 125:13868–13878, 2003 ) correlates methyl carbon and proton shifts to C γ , C β , and C α…”
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A Variable Light Domain Fluorogen Activating Protein Homodimerizes To Activate Dimethylindole Red by Senutovitch, Nina, Stanfield, Robyn L, Bhattacharyya, Shantanu, Rule, Gordon S, Wilson, Ian A, Armitage, Bruce A, Waggoner, Alan S, Berget, Peter B

“…Novel fluorescent tools such as green fluorescent protein analogues and fluorogen activating proteins (FAPs) are useful in biological imaging for tracking…”
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