In vitro and in vivo biological performance of hydroxyapatite from fish waste

The aim of this study was to evaluate biocompatibility of hydroxyapatite (HAP) from fish waste using in vitro and in vivo assays. Fish samples (whitemouth croaker - Micropogonias furnieri ) from the biowaste was used as HAP source. Pre-osteoblastic MC3T3-E1 cells were used in vitro study. In additio...

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Bibliographic Details
Published in:	Journal of materials science. Materials in medicine Vol. 32; no. 9; p. 109
Main Authors:	Prado, João Paulo dos Santos, Yamamura, Hirochi, Magri, Angela Maria Paiva, Ruiz, Pedro Luiz Muniz, Prado, José Lucas dos Santos, Rennó, Ana Claudia Muniz, Ribeiro, Daniel Araki, Granito, Renata Neves
Format:	Journal Article
Language:	English
Published:	New York Springer US 01-09-2021 Springer Nature B.V Springer
Subjects:	Animals Biocompatibility Biocompatibility Studies Biomaterials Biomedical Engineering and Bioengineering Biomedical materials Biotechnology Blood vessels Bone biomaterials Bone Regeneration - drug effects Bone Substitutes - chemistry Bone Substitutes - isolation & purification Bone Substitutes - pharmacology Calvaria Cell Differentiation - drug effects Cell proliferation Cell Proliferation - drug effects Cells, Cultured Ceramics Chemistry and Materials Science Composites Cytotoxicity Defects Deoxyribonucleic acid DNA Durapatite - isolation & purification Durapatite - pharmacology Exposure Fish Fish Products - analysis Glass Granulation Hydroxyapatite Implantation In vivo methods and tests Inflammation Materials Science Materials Testing Mice Micropogonias furnieri Natural Materials Osteoblasts Osteoblasts - cytology Osteoblasts - drug effects Osteoblasts - physiology Osteogenesis - drug effects Perciformes Polymer Sciences Polymerase chain reaction Rats Regenerative Medicine/Tissue Engineering Skull - drug effects Skull - physiology Solid Waste - analysis Surfaces and Interfaces Surgical implants Thin Films Tissue engineering Toxicity
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Summary:	The aim of this study was to evaluate biocompatibility of hydroxyapatite (HAP) from fish waste using in vitro and in vivo assays. Fish samples (whitemouth croaker - Micropogonias furnieri ) from the biowaste was used as HAP source. Pre-osteoblastic MC3T3-E1 cells were used in vitro study. In addition, bone defects were artificially created in rat calvaria and filled with HAP in vivo. The results demonstrated that HAP reduced cytotoxicity in pre-osteoblast cells after 3 and 6 days following HAP exposure. DNA concentration was lower in the HAP group after 6 days. Quantitative RT-PCR did not show any significant differences ( p > 0.05) between groups. In vivo study revealed that bone defects filled with HAP pointed out moderate chronic inflammatory cells with slight proliferation of blood vessels after 7 and 15 days. Chronic inflammatory infiltrate was absent after 30 days of HAP exposure. There was also a decrease in the amount of biomaterial, being followed by newly formed bone tissue. All experimental groups also demonstrated strong RUNX-2 immoexpression in the granulation tissue as well as in cells in close contact with biomaterial. The number of osteoblasts inside the defect area was lower in the HAP group when compared to control group after 7 days post-implantation. Similarly, the osteoblast surface as well as the percentage of bone surface was higher in control group when compared with HAP group after 7 days post-implantation. Taken together, HAP from fish waste is a promising possibility that should be explored more carefully by tissue-engineering or biotechnology.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0957-4530 1573-4838
DOI:	10.1007/s10856-021-06591-x