Targeting ferritin receptors for the selective delivery of imaging and therapeutic agents to breast cancer cells

Targeting ferritin receptors for the selective delivery of imaging and therapeutic agents to breast cancer cells

In this work the selective uptake of native horse spleen ferritin and apoferritin loaded with MRI contrast agents has been assessed in human breast cancer cells (MCF-7 and MDA-MB-231). The higher expression of L-ferritin receptors (SCARA5) led to an enhanced uptake in MCF-7 as shown in T2 and T1 wei...

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Bibliographic Details
Published in:Nanoscale Vol. 7; no. 15; p. 6527
Main Authors: Geninatti Crich, S, Cadenazzi, M, Lanzardo, S, Conti, L, Ruiu, R, Alberti, D, Cavallo, F, Cutrin, J C, Aime, S
Format: Journal Article
Language:English
Published: England 21-04-2015
Subjects:
Agar - chemistry
Animals
Anti-Inflammatory Agents - chemistry
Antineoplastic Agents - administration & dosage
Apoferritins - chemistry
Breast Neoplasms - drug therapy
Cell Line, Tumor
Cell Proliferation
Contrast Media - chemistry
Curcumin - chemistry
Drug Carriers
Female
Ferritins - chemistry
Horses
Humans
Iron-Binding Proteins - chemistry
Magnetic Resonance Imaging
MCF-7 Cells
Receptors, Cell Surface - chemistry
Scavenger Receptors, Class A - metabolism
Spleen - metabolism
Temperature
Theranostic Nanomedicine
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Summary:In this work the selective uptake of native horse spleen ferritin and apoferritin loaded with MRI contrast agents has been assessed in human breast cancer cells (MCF-7 and MDA-MB-231). The higher expression of L-ferritin receptors (SCARA5) led to an enhanced uptake in MCF-7 as shown in T2 and T1 weighted MR images, respectively. The high efficiency of ferritin internalization in MCF-7 has been exploited for the simultaneous delivery of curcumin, a natural therapeutic molecule endowed with antineoplastic and anti-inflammatory action, and the MRI contrast agent Gd-HPDO3A. This theranostic system is able to treat selectively breast cancer cells over-expressing ferritin receptors. By entrapping in apoferritin both Gd-HPDO3A and curcumin, it was possible to deliver a therapeutic dose of 167 μg ml(-1) (as calculated by MRI) of this natural drug to MCF-7 cells, thus obtaining a significant reduction of cell proliferation.
ISSN:2040-3372
DOI:10.1039/c5nr00352k