Search Results - "Rudolph, Rainer"

Crystal Structure of the Ligand-bound Glucagon-like Peptide-1 Receptor Extracellular Domain by Runge, Steffen, Thøgersen, Henning, Madsen, Kjeld, Lau, Jesper, Rudolph, Rainer

“…The glucagon-like peptide-1 receptor (GLP-1R) belongs to Family B1 of the seven-transmembrane G protein-coupled receptors, and its natural agonist ligand is…”
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Suppression of protein aggregation by L-arginine by Lange, Christian, Rudolph, Rainer

“…L-Arginine is one of the most commonly used and most generally applicable suppressors of protein aggregation. Its effect as enhancer of in vitro protein…”
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Passing the baton in class B GPCRs: peptide hormone activation via helix induction? by Parthier, Christoph, Reedtz-Runge, Steffen, Rudolph, Rainer, Stubbs, Milton T.

“…G-protein-coupled receptors (GPCRs) represent the largest constellation of validated drug targets. Crystal structures of class A GPCRs have facilitated major…”
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Crystal Structure of Glucagon-like Peptide-1 in Complex with the Extracellular Domain of the Glucagon-like Peptide-1 Receptor by Underwood, Christina Rye, Garibay, Patrick, Knudsen, Lotte Bjerre, Hastrup, Sven, Peters, Günther H., Rudolph, Rainer, Reedtz-Runge, Steffen

“…GLP-1 (glucagon-like peptide-1) is an incretin released from intestinal L-cells in response to food intake. Activation of the GLP-1 receptor potentiates the…”
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Ionic liquids as refolding additives: Variation of the anion by Buchfink, Robert, Tischer, Alexander, Patil, Ganesh, Rudolph, Rainer, Lange, Christian

“…Several ionic liquids have been shown to act as suppressors of protein aggregation and to effectively promote the in vitro refolding of denatured proteins. In…”
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New binding mode to TNF-alpha revealed by ubiquitin-based artificial binding protein by Hoffmann, Andreas, Kovermann, Michael, Lilie, Hauke, Fiedler, Markus, Balbach, Jochen, Rudolph, Rainer, Pfeifer, Sven

“…A variety of approaches have been employed to generate binding proteins from non-antibody scaffolds. Utilizing a beta-sheet of the human ubiquitin for paratope…”
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Ionic liquids as refolding additives: N′‐alkyl and N′‐(ω‐hydroxyalkyl) N‐methylimidazolium chlorides by Lange, Christian, Patil, Ganesh, Rudolph, Rainer

“…The purpose of this work was to investigate the influence of a series of N′‐alkyl and N′‐(ω‐hydroxy‐alkyl)‐N‐methylimidazolium chlorides on the renaturation of…”
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Crystal structure of the incretin-bound extracellular domain of a G protein-coupled receptor by Parthier, Christoph, Kleinschmidt, Martin, Neumann, Piotr, Rudolph, Rainer, Manhart, Susanne, Schlenzig, Dagmar, Fanghänel, Jörg, Rahfeld, Jens-Ulrich, Demuth, Hans-Ulrich, Stubbs, Milton T

“…Incretins, endogenous polypeptide hormones released in response to food intake, potentiate insulin secretion from pancreatic β cells after oral glucose…”
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l‐Arginine increases the solubility of unfolded species of hen egg white lysozyme by Reddy K., Ravi Charan, Lilie, Hauke, Rudolph, Rainer, Lange, Christian

“…l‐Arginine (l‐Arg) has been widely used as an enhancer of protein renaturation. The mechanism behind its action is still not fully understood. Using hen egg…”
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Advances in refolding of proteins produced in E. coli by Lilie, Hauke, Schwarz, Elisabeth, Rudolph, Rainer

“…Inclusion body production is a common theme in recombinant protein technology. Hence, renaturation of these inclusion body proteins is a field of increasing…”
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In vitro folding of inclusion body proteins by Rudolph, R, Lilie, H

“…Insoluble, inactive inclusion bodies are frequently formed upon recombinant protein production in transformed microorganisms. These inclusion bodies, which…”
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Artificial, non-antibody binding proteins for pharmaceutical and industrial applications by Hey, Thomas, Fiedler, Erik, Rudolph, Rainer, Fiedler, Markus

“…Using combinatorial chemistry to generate novel binding molecules based on protein frameworks (‘scaffolds’) is a concept that has been strongly promoted during…”
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Ionic Liquids and Proteases: A Clean Alliance for Semisynthesis by Wehofsky, Nicole, Wespe, Christian, Cerovsky, Vaclav, Pech, Andreas, Hoess, Eva, Rudolph, Rainer, Bordusa, Frank

“…Herein we present the first report on protease-catalysed ligation of cleavage-sensitive peptide and protein fragments in ionic-liquid-containing solvent…”
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Mammalian G protein-coupled receptor expression in Escherichia coli: II. Refolding and biophysical characterization of mouse cannabinoid receptor 1 and human parathyroid hormone receptor 1 by Michalke, Kerstin, Huyghe, Céline, Lichière, Julie, Gravière, Marie-Eve, Siponen, Marina, Sciara, Giuliano, Lepaul, Isabelle, Wagner, Renaud, Magg, Christine, Rudolph, Rainer, Cambillau, Christian, Desmyter, Aline

“…G protein-coupled receptors (GPCRs) represent approximately 3% of the human proteome. They are involved in a large number of diverse processes and, therefore,…”
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Biophysical Comparison of BMP-2, ProBMP-2, and the Free Pro-peptide Reveals Stabilization of the Pro-peptide by the Mature Growth Factor by Hillger, Frank, Herr, Gerhard, Rudolph, Rainer, Schwarz, Elisabeth

“…Pro-forms of growth factors have received intensive scientific attention recently because in some cases different biological activities have been ascribed…”
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Inhibition of aggregation side reactions during in vitro protein folding by De Bernardez Clark, E, Schwarz, E, Rudolph, R

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Identification of a Disulfide Bridge Essential for Transport Function of the Human Proton-coupled Amino Acid Transporter hPAT1 by Dorn, Madlen, Weiwad, Matthias, Markwardt, Fritz, Laug, Linda, Rudolph, Rainer, Brandsch, Matthias, Bosse-Doenecke, Eva

“…The proton-coupled amino acid transporter 1 (PAT1, SLC36A1) mediates the uptake of small neutral amino acids at the apical membrane of intestinal epithelial…”
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The role of N-glycosylation in transport function and surface targeting of the human solute carrier PAT1 by Dorn, Madlen, Jaehme, Michael, Weiwad, Matthias, Markwardt, Fritz, Rudolph, Rainer, Brandsch, Matthias, Bosse-Doenecke, Eva

“…In the present study we show in the Xenopus laevis expression system that the proton-coupled amino acid transporter 1 (PAT1, SLC36A1) is glycosylated at…”
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Mammalian G-protein-coupled receptor expression in Escherichia coli: I. High-throughput large-scale production as inclusion bodies by Michalke, Kerstin, Gravière, Marie-Eve, Huyghe, Céline, Vincentelli, Renaud, Wagner, Renaud, Pattus, Franc, Schroeder, Kathrin, Oschmann, Jan, Rudolph, Rainer, Cambillau, Christian, Desmyter, Aline

“…G-protein-coupled receptors (GPCRs) represent approximately 3% of human proteome and the most prominent class of pharmacological targets. Despite their…”
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The Isolated N-terminal Domain of the Glucagon-like Peptide-1 (GLP-1) Receptor Binds Exendin Peptides with Much Higher Affinity than GLP-1 by López de Maturana, Rakel, Willshaw, Angela, Kuntzsch, Antje, Rudolph, Rainer, Donnelly, Dan

“…Two fragments of the receptor for glucagon-like peptide-1 (GLP-1), each containing the N-terminal domain, were expressed and characterized in either bacterial…”
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