D-glucose overflow metabolism in an evolutionary engineered high-performance D-xylose consuming Saccharomyces cerevisiae strain

D-glucose overflow metabolism in an evolutionary engineered high-performance D-xylose consuming Saccharomyces cerevisiae strain

ABSTRACT Co-consumption of D-xylose and D-glucose by Saccharomyces cerevisiae is essential for cost-efficient cellulosic bioethanol production. There is a need for improved sugar conversion rates to minimize fermentation times. Previously, we have employed evolutionary engineering to enhance D-xylos...

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Bibliographic Details
Published in:FEMS yeast research Vol. 21; no. 1; pp. 1 - 14
Main Authors: Nijland, Jeroen G, Shin, Hyun Yong, Dore, Eleonora, Rudinatha, Donny, de Waal, Paul P, Driessen, Arnold J M
Format: Journal Article
Language:English
Published: England Oxford University Press 01-02-2021
Subjects:
Biofuels
Dextrose
Ethanol
Fermentation
Glucose
Glucose metabolism
Glycolysis
Hexokinase
Metabolism
Parenting
Phosphates
Physiological aspects
Saccharomyces cerevisiae
Scientific equipment and supplies industry
Trehalose
Trehalose-6-phosphate
Xylose
Yeast
United States
Netherlands
sugar transport
bioethanol
trehalose-6-phosphate
yeast
D-xylose transporter
glycolysis
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Summary:ABSTRACT Co-consumption of D-xylose and D-glucose by Saccharomyces cerevisiae is essential for cost-efficient cellulosic bioethanol production. There is a need for improved sugar conversion rates to minimize fermentation times. Previously, we have employed evolutionary engineering to enhance D-xylose transport and metabolism in the presence of D-glucose in a xylose-fermenting S. cerevisiae strain devoid of hexokinases. Re-introduction of Hxk2 in the high performance xylose-consuming strains restored D-glucose utilization during D-xylose/D-glucose co-metabolism, but at rates lower than the non-evolved strain. In the absence of D-xylose, D-glucose consumption was similar to the parental strain. The evolved strains accumulated trehalose-6-phosphate during sugar co-metabolism, and showed an increased expression of trehalose pathway genes. Upon the deletion of TSL1, trehalose-6-phosphate levels were decreased and D-glucose consumption and growth on mixed sugars was improved. The data suggest that D-glucose/D-xylose co-consumption in high-performance D-xylose consuming strains causes the glycolytic flux to saturate. Excess D-glucose is phosphorylated enters the trehalose pathway resulting in glucose recycling and energy dissipation, accumulation of trehalose-6-phosphate which inhibits the hexokinase activity, and release of trehalose into the medium. Balanced D-glucose and D-xylose metabolism in evolutionary engineered yeast strains.
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ISSN:1567-1364
1567-1356
1567-1364
DOI:10.1093/femsyr/foaa062