Carbon dioxide concentrations are very high in developing oilseeds

A new method has been developed to rapidly determine the total inorganic carbon concentration (gaseous [CO 2] + aqueous [CO 2] + [HCO 3 –] + [CO 3 2–]) in developing seeds. Seeds are rapidly dissected and homogenized in 1 N HCl in gas-tight vials. The headspace gas is then analyzed by infrared gas a...

Full description

Saved in:

Bibliographic Details
Published in:	Plant physiology and biochemistry Vol. 42; no. 9; pp. 703 - 708
Main Authors:	Goffman, Fernando D., Ruckle, Mike, Ohlrogge, John, Shachar-Hill, Yair
Format:	Journal Article
Language:	English
Published:	Paris Elsevier Masson SAS 01-09-2004 Elsevier
Subjects:	Biological and medical sciences Brassica rapa - chemistry Brassica rapa - growth & development Carbon dioxide Carbon Dioxide - analysis Carbon Dioxide - metabolism Carbon economy CO 2 refixation Developing oilseeds Fundamental and applied biological sciences. Psychology Gases - analysis Glycine max - chemistry Glycine max - growth & development Hydrogen-Ion Concentration Metabolism Metabolism. Physicochemical requirements Plant physiology and development Rapeseed Seeds - chemistry Seeds - growth & development Soybean Spectrophotometry, Infrared CO 2 refixation CO 2 (aq.) IRGA Carbon dioxide pCO 2 DAF Rubisco Soybean S.E CO 2 (g) Rapeseed PDH Carbon economy Developing oilseeds FAS Seeds Lipids Biosynthesis Glycine max Hydrogencarbonates Leguminosae Cruciferae Dicotyledones Angiospermae Brassica napus Spermatophyta Carbonates Biological accumulation CO2 refixation
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	A new method has been developed to rapidly determine the total inorganic carbon concentration (gaseous [CO 2] + aqueous [CO 2] + [HCO 3 –] + [CO 3 2–]) in developing seeds. Seeds are rapidly dissected and homogenized in 1 N HCl in gas-tight vials. The headspace gas is then analyzed by infrared gas analysis. Developing rapeseed ( Brassica napus L.) and soybean [ Glycine max (L.) Merr.] seeds were analyzed and found to have up to 40 and 12 mM total inorganic carbon, respectively. These concentrations are ca. 600–2000-fold higher than in ambient air or values reported for leaves. Carbon dioxide concentrations in rapeseed peaked during the stage of maximum oil synthesis and declined as seeds matured. The consequences for seed metabolism, physiology and carbon economy are discussed.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0981-9428 1873-2690
DOI:	10.1016/j.plaphy.2004.07.003