Search Results - "Rowe, Jacob B"

High‐throughput CRISPR for Generating Therapeutic Nanobodies by Rowe, Jacob B., Lee, Kyutae, Isom, Daniel

“…Engineered antibodies have had a widespread impact throughout biomedical sciences, from their use as fundamental research tools to their employment as…”
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Predictable cholesterol binding sites in GPCRs lack consensus motifs by Taghon, Geoffrey J., Rowe, Jacob B., Kapolka, Nicholas J., Isom, Daniel G.

“…A rich diversity of transmembrane G protein-coupled receptors (GPCRs) are used by eukaryotes to sense physical and chemical signals. In humans alone, 800 GPCRs…”
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The evolution and mechanism of GPCR proton sensing by Rowe, Jacob B., Kapolka, Nicholas J., Taghon, Geoffrey J., Morgan, William M., Isom, Daniel G.

“…Of the 800 G protein–coupled receptors (GPCRs) in humans, only three (GPR4, GPR65, and GPR68) regulate signaling in acidified microenvironments by sensing…”
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Location-biased activation of the proton-sensor GPR65 is uncoupled from receptor trafficking by Morales Rodríguez, Loyda M, Crilly, Stephanie E, Rowe, Jacob B, Isom, Daniel G, Puthenveedu, Manojkumar A

“…The canonical view of G protein-coupled receptor (GPCR) function is that receptor trafficking is tightly coupled to signaling. GPCRs remain on the plasma…”
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Proton-gated coincidence detection is a common feature of GPCR signaling by Kapolka, Nicholas J., Rowe, Jacob B., Taghon, Geoffrey J., Morgan, William M., O’Shea, Corin R., Isom, Daniel G.

“…The evolutionary expansion of G protein-coupled receptors (GPCRs) has produced a rich diversity of transmembrane sensors for many physical and chemical…”
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CRISPR-addressable yeast strains with applications in human G protein–coupled receptor profiling and synthetic biology by Rowe, Jacob B., Taghon, Geoffrey J., Kapolka, Nicholas J., Morgan, William M., Isom, Daniel G.

“…Genome stability is essential for engineering cell-based devices and reporter systems. With the advent of CRISPR technology, it is now possible to build such…”
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Metastability Gap in the Phase Diagram of Monoclonal IgG Antibody by Rowe, Jacob B., Cancel, Rachel A., Evangelous, Tyler D., Flynn, Rhiannon P., Pechenov, Sergei, Subramony, J. Anand, Zhang, Jifeng, Wang, Ying

“…Crystallization of IgG antibodies has important applications in the fields of structural biology, biotechnology, and biopharmaceutics. However, a rational…”
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Submicron Aggregation of Chemically Denatured Monoclonal Antibody by Rowe, Jacob B, Flynn, Rhiannon P, Wooten, Harrison R, Noufer, Hailey A, Cancel, Rachel A, Zhang, Jifeng, Subramony, J. Anand, Pechenov, Sergei, Wang, Ying

“…Isothermal chemical denaturation (ICD) has been widely used to evaluate the conformational stability of therapeutic proteins such as monoclonal antibodies…”
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The evolution and mechanism of GPCR proton sensing by Rowe, Jacob B, Kapolka, Nicholas J, Taghon, Geoffrey J, Morgan, William M, Isom, Daniel G

“…Of the 800 G protein-coupled receptors (GPCRs) in humans, only three (GPR4, GPR65, and GPR68) regulate signaling in acidified microenvironments by sensing…”
Get full text