Search Results - "Rouet, Romain"

Stability engineering of the human antibody repertoire by Rouet, Romain, Lowe, David, Christ, Daniel

“…Human monoclonal antibodies often display limited thermodynamic and colloidal stabilities. This behavior hinders their production, and places limitations on…”
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Efficient Intracellular Delivery of CRISPR-Cas Ribonucleoproteins through Receptor Mediated Endocytosis by Rouet, Romain, Christ, Daniel

“…We recently reported a new delivery system harnessing surface receptors for targeted uptake of CRISPR-Cas9 ribonucleoprotein into mammalian cells (Rouet et…”
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CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing by Moreno-Mateos, Miguel A., Fernandez, Juan P., Rouet, Romain, Vejnar, Charles E., Lane, Maura A., Mis, Emily, Khokha, Mustafa K., Doudna, Jennifer A., Giraldez, Antonio J.

“…Cpf1 is a novel class of CRISPR-Cas DNA endonucleases, with a wide range of activity across different eukaryotic systems. Yet, the underlying determinants of…”
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General strategy for the generation of human antibody variable domains with increased aggregation resistance by Dudgeon, Kip, Rouet, Romain, Kokmeijer, Iris, Schofield, Peter, Stolp, Jessica, Langley, David, Stock, Daniela, Christ, Daniel

“…The availability of stable human antibody reagents would be of considerable advantage for research, diagnostic, and therapeutic applications. Unfortunately,…”
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Redemption of autoantibodies on anergic B cells by variable-region glycosylation and mutation away from self-reactivity by Sabouri, Zahra, Schofield, Peter, Horikawa, Keisuke, Spierings, Emily, Kipling, David, Randall, Katrina L, Langley, David, Roome, Brendan, Vazquez-Lombardi, Rodrigo, Rouet, Romain, Hermes, Jana, Chan, Tyani D, Brink, Robert, Dunn-Walters, Deborah K, Christ, Daniel, Goodnow, Christopher C

“…The best-understood mechanisms for achieving antibody self/non-self discrimination discard self-reactive antibodies before they can be tested for binding…”
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Receptor-Mediated Delivery of CRISPR-Cas9 Endonuclease for Cell-Type-Specific Gene Editing by Rouet, Romain, Thuma, Benjamin A, Roy, Marc D, Lintner, Nathanael G, Rubitski, David M, Finley, James E, Wisniewska, Hanna M, Mendonsa, Rima, Hirsh, Ariana, de Oñate, Lorena, Compte Barrón, Joan, McLellan, Thomas J, Bellenger, Justin, Feng, Xidong, Varghese, Alison, Chrunyk, Boris A, Borzilleri, Kris, Hesp, Kevin D, Zhou, Kaihong, Ma, Nannan, Tu, Meihua, Dullea, Robert, McClure, Kim F, Wilson, Ross C, Liras, Spiros, Mascitti, Vincent, Doudna, Jennifer A

“…CRISPR-Cas RNA-guided endonucleases hold great promise for disrupting or correcting genomic sequences through site-specific DNA cleavage and repair. However,…”
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Next-Generation Sequencing of Antibody Display Repertoires by Rouet, Romain, Jackson, Katherine J L, Langley, David B, Christ, Daniel

“…selection technology has transformed the development of therapeutic monoclonal antibodies. Using methods such as phage, ribosome, and yeast display, high…”
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Broadly neutralizing SARS-CoV-2 antibodies through epitope-based selection from convalescent patients by Rouet, Romain, Henry, Jake Y., Johansen, Matt D., Sobti, Meghna, Balachandran, Harikrishnan, Langley, David B., Walker, Gregory J., Lenthall, Helen, Jackson, Jennifer, Ubiparipovic, Stephanie, Mazigi, Ohan, Schofield, Peter, Burnett, Deborah L., Brown, Simon H. J., Martinello, Marianne, Hudson, Bernard, Gilroy, Nicole, Post, Jeffrey J., Kelleher, Anthony, Jäck, Hans-Martin, Goodnow, Christopher C., Turville, Stuart G., Rawlinson, William D., Bull, Rowena A., Stewart, Alastair G., Hansbro, Philip M., Christ, Daniel

“…Emerging variants of concern (VOCs) are threatening to limit the effectiveness of SARS-CoV-2 monoclonal antibodies and vaccines currently used in clinical…”
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Long-term persistence of RBD+ memory B cells encoding neutralizing antibodies in SARS-CoV-2 infection by Abayasingam, Arunasingam, Balachandran, Harikrishnan, Agapiou, David, Hammoud, Mohamed, Rodrigo, Chaturaka, Keoshkerian, Elizabeth, Li, Hui, Brasher, Nicholas A., Christ, Daniel, Rouet, Romain, Burnet, Deborah, Grubor-Bauk, Branka, Rawlinson, William, Turville, Stuart, Aggarwal, Anupriya, Stella, Alberto Ospina, Fichter, Christina, Brilot, Fabienne, Mina, Michael, Post, Jeffrey J., Hudson, Bernard, Gilroy, Nicky, Dwyer, Dominic, Sasson, Sarah C., Tea, Fiona, Pilli, Deepti, Kelleher, Anthony, Tedla, Nicodemus, Lloyd, Andrew R., Martinello, Marianne, Bull, Rowena A.

“…Considerable concerns relating to the duration of protective immunity against severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) exist, with evidence…”
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High titre neutralizing antibodies in response to SARS-CoV-2 infection require RBD-specific CD4 T cells that include proliferative memory cells by Phetsouphanh, Chansavath, Khoo, Weng Hua, Jackson, Katherine, Klemm, Vera, Howe, Annett, Aggarwal, Anupriya, Akerman, Anouschka, Milogiannakis, Vanessa, Stella, Alberto Ospina, Rouet, Romain, Schofield, Peter, Faulks, Megan L, Law, Hannah, Danwilai, Thidarat, Starr, Mitchell, Munier, C Mee Ling, Christ, Daniel, Singh, Mandeep, Croucher, Peter I, Brilot-Turville, Fabienne, Turville, Stuart, Phan, Tri Giang, Dore, Gregory J, Darley, David, Cunningham, Philip, Matthews, Gail V, Kelleher, Anthony D, Zaunders, John J

“…Long-term immunity to SARS-CoV-2 infection, including neutralizing antibodies and T cell-mediated immunity, is required in a very large majority of the…”
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Potent SARS-CoV-2 binding and neutralization through maturation of iconic SARS-CoV-1 antibodies by Rouet, Romain, Mazigi, Ohan, Walker, Gregory J., Langley, David B., Sobti, Meghna, Schofield, Peter, Lenthall, Helen, Jackson, Jennifer, Ubiparipovic, Stephanie, Henry, Jake Y., Abayasingam, Arunasingam, Burnett, Deborah, Kelleher, Anthony, Brink, Robert, Bull, Rowena A., Turville, Stuart, Stewart, Alastair G., Goodnow, Christopher C., Rawlinson, William D., Christ, Daniel

“…Antibodies against coronavirus spike protein potently protect against infection and disease, but whether such protection can be extended to variant…”
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Bispecific antibodies with native chain structure by Rouet, Romain, Christ, Daniel

“…Two new methods overcome a major hurdle that blocked easy and efficient design and expression of human bispecific antibodies…”
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Antibody-mediated delivery of CRISPR-Cas9 ribonucleoproteins in human cells by Ubiparipovic, Stephanie, Christ, Daniel, Rouet, Romain

“…The CRISPR genome editing technology holds great clinical potential for the treatment of monogenetic disorders such as sickle cell disease. The therapeutic in…”
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I-motif DNA structures are formed in the nuclei of human cells by Zeraati, Mahdi, Langley, David B., Schofield, Peter, Moye, Aaron L., Rouet, Romain, Hughes, William E., Bryan, Tracy M., Dinger, Marcel E., Christ, Daniel

“…Human genome function is underpinned by the primary storage of genetic information in canonical B-form DNA, with a second layer of DNA structure providing…”
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Fully Human VH Single Domains That Rival the Stability and Cleft Recognition of Camelid Antibodies by Rouet, Romain, Dudgeon, Kip, Christie, Mary, Langley, David, Christ, Daniel

“…Human VH single domains represent a promising class of antibody fragments with applications as therapeutic modalities. Unfortunately, isolated human VH domains…”
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Bispecific antibodies with native chain structure: two new methods overcome a major hurdle that blocked easy and efficient design and expression of human bispecific antibodies by Rouet, Romain, Christ, Daniel

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Optimized CRISPR-Cpf1 system for genome editing in zebrafish by Fernandez, Juan P., Vejnar, Charles E., Giraldez, Antonio J., Rouet, Romain, Moreno-Mateos, Miguel A.

“…•Guide to design crRNA using CRISPRscan.org.•Step-by-step protocol to generate crRNAs and purifiy Cpf1 protein.•Detailed description on how to use Cpf1-crRNA…”
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Immunizations with diverse sarbecovirus receptor-binding domains elicit SARS-CoV-2 neutralizing antibodies against a conserved site of vulnerability by Burnett, Deborah L., Jackson, Katherine J.L., Langley, David B., Aggarwal, Anupriya, Stella, Alberto Ospina, Johansen, Matt D., Balachandran, Harikrishnan, Lenthall, Helen, Rouet, Romain, Walker, Gregory, Saunders, Bernadette M., Singh, Mandeep, Li, Hui, Henry, Jake Y., Jackson, Jennifer, Stewart, Alastair G., Witthauer, Franka, Spence, Matthew A., Hansbro, Nicole G., Jackson, Colin, Schofield, Peter, Milthorpe, Claire, Martinello, Marianne, Schulz, Sebastian R., Roth, Edith, Kelleher, Anthony, Emery, Sean, Britton, Warwick J., Rawlinson, William D., Karl, Rudolfo, Schäfer, Simon, Winkler, Thomas H., Brink, Robert, Bull, Rowena A., Hansbro, Philip M., Jäck, Hans-Martin, Turville, Stuart, Christ, Daniel, Goodnow, Christopher C.

“…Viral mutations are an emerging concern in reducing SARS-CoV-2 vaccination efficacy. Second-generation vaccines will need to elicit neutralizing antibodies…”
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Human genomic DNA is widely interspersed with i-motif structures by Peña Martinez, Cristian David, Zeraati, Mahdi, Rouet, Romain, Mazigi, Ohan, Henry, Jake Y, Gloss, Brian, Kretzmann, Jessica A, Evans, Cameron W, Ruggiero, Emanuela, Zanin, Irene, Marušič, Maja, Plavec, Janez, Richter, Sara N, Bryan, Tracy M, Smith, Nicole M, Dinger, Marcel E, Kummerfeld, Sarah, Christ, Daniel

“…DNA i-motif structures are formed in the nuclei of human cells and are believed to provide critical genomic regulation. While the existence, abundance, and…”
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The retroelement Lx9 puts a brake on the immune response to virus infection by Bartonicek, Nenad, Rouet, Romain, Warren, Joanna, Loetsch, Claudia, Rodriguez, Gabriela Santos, Walters, Stacey, Lin, Francis, Zahra, David, Blackburn, James, Hammond, Jillian M., Reis, Andre L. M., Deveson, Ira W., Zammit, Nathan, Zeraati, Mahdi, Grey, Shane, Christ, Daniel, Mattick, John S., Chtanova, Tatyana, Brink, Robert, Dinger, Marcel E., Weatheritt, Robert J., Sprent, Jonathan, King, Cecile

“…The notion that mobile units of nucleic acid known as transposable elements can operate as genomic controlling elements was put forward over six decades ago 1…”
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