Exploring valrubicin’s effect on Propionibacterium acnes-induced skin inflammation in vitro and in vivo

Acne is a common skin disease involving colonization with Propionibacterium acnes (P. acnes), hyperproliferation of the follicular epithelium and inflammatory events. Valrubicin is a second-generation anthracycline, non-toxic upon contact, and available in a topical formulation. Valrubicin’s predece...

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Bibliographic Details
Published in:	Dermatology reports Vol. 7; no. 3
Main Authors:	Rottboell, Louise, De Foenss, Sarah, Thomsen, Kenneth, Christiansen, Helle, Andersen, Stine M., Dam, Thomas N., Rosada, Cecilia, Stenderup, Karin
Format:	Journal Article
Language:	English
Published:	Pavia PAGEPress Publications 17-12-2015 PAGEPress Publications, Pavia, Italy
Subjects:	Acne Inflammation mouse model Propionibacterium acnes skin inflammation Tumor necrosis factor-TNF valrubicin
Online Access:	Get full text
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Summary:	Acne is a common skin disease involving colonization with Propionibacterium acnes (P. acnes), hyperproliferation of the follicular epithelium and inflammatory events. Valrubicin is a second-generation anthracycline, non-toxic upon contact, and available in a topical formulation. Valrubicin’s predecessor doxorubicin possesses antibacterial effects and previously we demonstrated that valrubicin inhibits keratinocyte proliferation and skin inflammation suggesting beneficial topical treatment of acne with valrubicin. This study aims to investigate valrubicin’s possible use in acne treatment by testing valrubicin’s antibacterial effects against P. acnes and P. acnes-induced skin inflammation in vitro and in vivo. Valrubicin was demonstrated not to possess antibacterial effects against P. acnes. Additionally, valrubicin was demonstrated not to reduce mRNA and protein expression levels of the inflammatory markers interleukin (IL)-1β, IL-8, and tumor necrosis factor (TNF)-α in vitro in human keratinocytes co-cultured with P. acnes. Moreover, in vivo, valrubicin, applied both topically and intra-dermally, was not able to reduce signs of inflammation in mouse ears intra-dermally injected with P. acnes. Taken together, this study does not support beneficial antibacterial and anti inflammatory effects of topical valrubicin treatment of acne.
Bibliography:	Contributions: LR, TND, CR, KS, conception of the work; LR, SdF, KT, HC, SMA, acquisition of data for the work; LR, TND, CR, KS, analysis and interpretation of data for the work; LR, SdF, KT, HC, SMA, TND, CR, KS, drafting the work and critically revising it for important intellectual content and final approval of the manuscript version to be published. Conflict of interest: LR, SdF, KT, HC, SMA, CR, declare no conflict of interest; KS received funding for operating expenses from Valderm ApS; TND is a consultant at Valderm ApS.
ISSN:	2036-7392 2036-7406
DOI:	10.4081/dr.2015.6246