Search Results - "Rothwell, Paul J."

A toolkit and benchmark study for FRET-restrained high-precision structural modeling by Kalinin, Stanislav, Peulen, Thomas, Sindbert, Simon, Rothwell, Paul J, Berger, Sylvia, Restle, Tobias, Goody, Roger S, Gohlke, Holger, Seidel, Claus A M

“…A collection of simulation tools and workflow for single-molecule Förster resonance energy transfer (smFRET) allows highly quantitative structural modeling…”
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A Pre-equilibrium before Nucleotide Binding Limits Fingers Subdomain Closure by Klentaq1 by Rothwell, Paul J., Waksman, Gabriel

“…Numerous studies have been undertaken to establish the mechanism of dNTP binding and template-directed incorporation by DNA polymerases. It has been…”
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Accurate single-molecule FRET studies using multiparameter fluorescence detection by Sisamakis, Evangelos, Valeri, Alessandro, Kalinin, Stanislav, Rothwell, Paul J, Seidel, Claus A M

“…In the recent decade, single-molecule (sm) spectroscopy has come of age and is providing important insight into how biological molecules function. So far our…”
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dbDNA™: An advanced platform for genetic medicines by Adie, Tom, Orefo, Ifey, Kysh, Daniel, Kondas, Katalin, Thapa, Shyam, Extance, Jon, Duncan, Tommy, Rothwell, Paul J.

“…•Touchlight has developed dbDNA for the scalable generation of high-quality GMP DNA free of bacterial sequences.•dbDNA has been proven both an enabling…”
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Linear doggybone DNA vaccine induces similar immunological responses to conventional plasmid DNA independently of immune recognition by TLR9 in a pre-clinical model by Allen, Alex, Wang, Chuan, Caproni, Lisa J., Sugiyarto, Gessa, Harden, Elena, Douglas, Leon R., Duriez, Patrick J., Karbowniczek, Kinga, Extance, Jon, Rothwell, Paul J., Orefo, Ifeayinwa, Tite, John P., Stevenson, Freda K., Ottensmeier, Christian H., Savelyeva, Natalia

“…Vaccination with DNA that encodes cancer antigens is a simple and convenient way to raise immunity against cancer and has already shown promise in the clinical…”
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Motions of the Fingers Subdomain of Klentaq1 Are Fast and Not Rate Limiting: Implications for the Molecular Basis of Fidelity in DNA Polymerases by Rothwell, Paul J., Mitaksov, Vesselin, Waksman, Gabriel

“…Various kinetic studies on nucleotide incorporation by DNA polymerases have established that a rate-limiting step occurs that is crucial in the mechanism of…”
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dNTP-dependent conformational transitions in the fingers subdomain of Klentaq1 DNA polymerase: insights into the role of the "nucleotide-binding" state by Rothwell, Paul J, Allen, William J, Sisamakis, Evangelos, Kalinin, Stanislav, Felekyan, Suren, Widengren, Jerker, Waksman, Gabriel, Seidel, Claus A M

“…Conformational selection plays a key role in the polymerase cycle. Klentaq1 exists in conformational equilibrium between three states (open, closed, and…”
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Single-molecule multiparameter fluorescence spectroscopy reveals directional MutS binding to mismatched bases in DNA by Cristóvão, Michele, Sisamakis, Evangelos, Hingorani, Manju M, Marx, Andreas D, Jung, Caroline P, Rothwell, Paul J, Seidel, Claus A M, Friedhoff, Peter

“…Mismatch repair (MMR) corrects replication errors such as mismatched bases and loops in DNA. The evolutionarily conserved dimeric MMR protein MutS recognizes…”
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An intramolecular FRET system monitors fingers subdomain opening in Klentaq1 by Allen, William J., Rothwell, Paul J., Waksman, Gabriel

“…A major goal of polymerase research is to determine the mechanism through which a nucleotide complementary to a templating DNA base is selected and delivered…”
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Novel synthetic plasmid and Doggybone™ DNA vaccines induce neutralizing antibodies and provide protection from lethal influenza challenge in mice by Scott, Veronica L, Patel, Ami, Villarreal, Daniel O, Hensley, Scott E, Ragwan, Edwin, Yan, Jian, Sardesai, Niranjan Y, Rothwell, Paul J, Extance, Jonathan P, Caproni, Lisa J, Weiner, David B

“…Nucleic acid-based vaccines (NAVs) are a promising alternative to conventional influenza vaccines with the potential to increase influenza vaccine availability…”
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Structure and mechanism of DNA polymerases by Rothwell, Paul J., Waksman, Gabriel

“…DNA polymerases are molecular motors directing the synthesis of DNA from nucleotides. All polymerases have a common architectural framework consisting of three…”
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dNTP-dependent Conformational Transitions in the Fingers Subdomain of Klentaq1 DNA Polymerase by Rothwell, Paul J., Allen, William J., Sisamakis, Evangelos, Kalinin, Stanislav, Felekyan, Suren, Widengren, Jerker, Waksman, Gabriel, Seidel, Claus A.M.

“…DNA polymerases are responsible for the accurate replication of DNA. Kinetic, single-molecule, and x-ray studies show that multiple conformational states are…”
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High Precision FRET to Determine Dynamic Protein Structures by Seidel, Claus A., Kalinin, Stanislav, Sindbert, Simon, Peulen, Thomas, Ajaj, Yathrib, Rothwell, Paul J., Hengstenberg, Carola, Herrmann, Christian

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Single Molecule FRET Studies of Protein Conformational Landscapes: 3 Prototypic Examples for the Relation Between Conformational Dynamics and Function by Richert, Markus, Rodnin, Dymitro, Hengstenberg, Carola S., Peulen, Thomas, Valeri, Alessandro, Rothwell, Paul J., Millon, Pohl, Rodnina, Marina, Herrmann, Christian, Seidel, Claus A.M.

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dNTP-Dependent Conformational Transitions in the Fingers Subdomain of Klentaq1 by Rothwell, Paul J., Allen, William J., Sisamakis, Evangelos, Janissen, Richard, Kalinin, Stanislav, Felekyan, Suren, Widengren, Jerker, Waksman, Gabriel, Seidel, Claus A.M.

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A Fluorescence Spectroscopic Approach To Quantify The Binding Characteristics Of The Intracellular Domain Of Crumbs With Pdz Domain-containing Proteins In Drosophila Melanogaster by Hornen, Heike, Schwarz, Christian, Rothwell, Paul J., Knust, Elisabeth, Seidel, Claus A.M.

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Single Molecule Fret Reveals Novel Dynamic Structure And Stoichiometry Of L27 Domain-mediated Polarity Complexes Formed By Drosophila Sdt/DPatj/DLin-7 by Renner, Andreas, Felekyan, Suren, Hornen, Heike, Rothwell, Paul J., Kalinin, Stanislav, Bachmann, André, Knust, Elisabeth, Seidel, Claus A.M.

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