Search Results - "Rossano, Adam J."

Expression of multiple transgenes from a single construct using viral 2A peptides in Drosophila by Daniels, Richard W, Rossano, Adam J, Macleod, Gregory T, Ganetzky, Barry

“…Expression of multiple reporter or effector transgenes in the same cell from a single construct is increasingly necessary in various experimental paradigms…”
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High-Probability Neurotransmitter Release Sites Represent an Energy-Efficient Design by Lu, Zhongmin, Chouhan, Amit K., Borycz, Jolanta A., Lu, Zhiyuan, Rossano, Adam J., Brain, Keith L., Zhou, You, Meinertzhagen, Ian A., Macleod, Gregory T.

“…Nerve terminals contain multiple sites specialized for the release of neurotransmitters. Release usually occurs with low probability, a design thought to…”
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Genetically encoded pH‐indicators reveal activity‐dependent cytosolic acidification of Drosophila motor nerve termini in vivo by Rossano, Adam J., Chouhan, Amit K., Macleod, Gregory T.

“…Key points •  Changes in pH occur within neurons during nerve activity and in response to hypoxic insult. •  Many aspects of neurophysiology are potentially…”
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Na+/H+ exchange via the Drosophila vesicular glutamate transporter mediates activity‐induced acid efflux from presynaptic terminals by Rossano, Adam J., Kato, Akira, Minard, Karyl I., Romero, Michael F., Macleod, Gregory T.

“…Key points Intracellular pH regulation is vital to neurons as nerve activity produces large and rapid acid loads in presynaptic terminals. Rapid clearance of…”
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Acid-Base Basics by Romero, Michael F., Rossano, Adam J.

“…Although students initially learn of ionic buffering in basic chemistry, buffering and acid-base transport in biology often is relegated to specialized…”
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pHire: A Novel Genetically Encoded Red Intracellular pH Sensor Expressed in Cells and Flies by Schreiber, Areonna C., Rossano, Adam J., Reynolds, Carmen J., Holmes, Heather L., Brown, Matthew R., Matveyenko, Aleksey, Romero, Michael F.

“…Intracellular pH regulation is vital for normal cellular function and is controlled by membrane H+ and HCO3‑ transporters. Most pH reporters have green…”
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Optical Quantification of Intracellular pH in Drosophila melanogaster Malpighian Tubule Epithelia with a Fluorescent Genetically-encoded pH Indicator by Rossano, Adam J, Romero, Michael F

“…Epithelial ion transport is vital to systemic ion homeostasis as well as maintenance of essential cellular electrochemical gradients. Intracellular pH (pHi) is…”
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Loading Drosophila nerve terminals with calcium indicators by Rossano, Adam J, Macleod, Gregory T

“…Calcium plays many roles in the nervous system but none more impressive than as the trigger for neurotransmitter release, and none more profound than as the…”
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Ex Vivo Quantification of pH i in Drosophila Malpighian Tubule Principal Cells Reveals Basolateral H + Equivalent—Coupled Oxalate Transport through Likely Slc26a1 Ortholog by Rossano, Adam J., Anderson, Jacob B., Romero, Michael F.

“…Abstract only Nephrolithiasis is a painful and costly healthcare complication. The most common kidney stones are composed of calcium oxalate and thus renal…”
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Optical Quantification of Intracellular pH in Drosophila melanogaster Malpighian Tubule Epithelia with a Fluorescent Genetically-encoded pH Indicator by Rossano, Adam J., Romero, Michael F.

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Characterization of pH i Regulation by NBCe1/SLC4A4 Variants with Known Clinical Phenotypes in Human Immortalized Trabecular Meshwork Cells with a Red‐Shifted Genetically‐Encoded pH‐Indicator “pHire” by Rossano, Adam J, Caldas Font, Paola N., Holmes, Heather L, Romero, Michael F

“…Abstract only The electrogenic Na + /HCO 3 − cotransporter (NBCe1/SLC4A4) maintains systemic acid/base balance by reabsorbing HCO 3 − in the renal proximal…”
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Na super(+)/H super(+) exchange via the Drosophila vesicular glutamate transporter mediates activity-induced acid efflux from presynaptic terminals by Rossano, Adam J, Kato, Akira, Minard, Karyl I, Romero, Michael F, Macleod, Gregory T

“…Key points * Intracellular pH regulation is vital to neurons as nerve activity produces large and rapid acid loads in presynaptic terminals. * Rapid clearance…”
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Loading Drosophila Nerve Terminals with Calcium Indicators by Rossano, Adam J., Macleod, Gregory T.

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