Effect of Methylene Blue on the Hemodynamic Instability Resulting From Liver Ischemia and Reperfusion in Rabbits

Abstract The experimental investigation was performed to study the effects of methylene blue (MB) on hemodynamic, biochemical, and tissue changes among rabbits undergoing liver ischemia and reperfusion (IR). Twenty-four rabbits were randomized into 5 groups: 1, SHAM, control; 2, MB infusion bolus (3...

Full description

Saved in:

Bibliographic Details
Published in:	Transplantation proceedings Vol. 43; no. 10; pp. 3643 - 3651
Main Authors:	Rosique, R.G, Rosique, M.J.F, Rosique, I.A, Tirapelli, L.F, Castro e Silva, O, dos Santos, J.S, Evora, P.R.B
Format:	Journal Article
Language:	English
Published:	Amsterdam Elsevier Inc 01-12-2011 Elsevier
Subjects:	Animals Biological and medical sciences Biomarkers - blood Blood Gas Analysis Cardiovascular Agents - pharmacology Disease Models, Animal Fundamental and applied biological sciences. Psychology Fundamental immunology Gastroenterology. Liver. Pancreas. Abdomen Hemodynamics - drug effects Liver - blood supply Liver - drug effects Liver - pathology Liver Circulation - drug effects Liver. Biliary tract. Portal circulation. Exocrine pancreas Male Medical sciences Methylene Blue - pharmacology Other diseases. Semiology Primary Graft Dysfunction - blood Primary Graft Dysfunction - drug therapy Primary Graft Dysfunction - pathology Primary Graft Dysfunction - physiopathology Rabbits Reperfusion Injury - blood Reperfusion Injury - drug therapy Reperfusion Injury - pathology Reperfusion Injury - physiopathology Surgery Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Time Factors Tissue, organ and graft immunology Ischemia reperfusion Rabbit Cardiovascular disease Hepatic disease Transplantation Lagomorpha Methylene blue Vascular disease Medicine Vertebrata Mammalia Reperfusion Treatment Surgery Animal Digestive diseases Graft Instability Hemodynamics Liver ischemia
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Abstract The experimental investigation was performed to study the effects of methylene blue (MB) on hemodynamic, biochemical, and tissue changes among rabbits undergoing liver ischemia and reperfusion (IR). Twenty-four rabbits were randomized into 5 groups: 1, SHAM, control; 2, MB infusion bolus (3 mg/kg); 3, IR, hepatic ischemia for 60 minutes followed by 120 minutes of reperfusion; 4, MB-R, undergoing ischemia that had received an MB bolus infusion (3 mg/kg) prior to reperfusion; 5, R-MB, undergoing ischemia and MB bolus infusion after hemodynamic instability caused by reperfusion. The analysis included continuous recording of vital signs. Blood samples were collected at 0, 60, and 180 minutes of IR to determine blood gases as well as biochemical markers of liver function, nitric oxide, lipid peroxidation, and neutrophil activity. At the end of each experiment, liver tissue samples were collected for histological evaluation of parenchymae markers. Statistical analysis used two-way analysis of variance (ANOVA) tests with significance set at P < .05. Vital signs significantly improved with MB infusion, irrespective of whether it was applied before or after reperfusion. Blood gas data revealed different patterns among the SHAM, MB, IR, MB-R, and R-MB groups, without statistical significance, except for favorable lactate results in the R-MB group ( P < .01), which displayed greater survival. Biochemical tests did not show significant differences among the groups, whereas histological analysis revealed favorable appearances for the MB-R and R-MB groups. The MB effect lasted long after reperfusion, suggesting that improvement in the hemodynamic parameters was not based on liver integrity, but rather was possibly related to endothelial function.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0041-1345 1873-2623
DOI:	10.1016/j.transproceed.2011.08.108